Image cytometry-based detection of aneuploidy by fluorescence in situ hybridization in suspension.

Cytogenetic abnormalities are important diagnostic and prognostic criteria for hematologic malignancies. Karyotyping and fluorescence in situ hybridization (FISH) are the conventional methods by which these abnormalities are detected. The sensitivity of these microscopy-based methods is limited by the abundance of the abnormal cells in the samples and therefore these analyses are commonly not applicable to minimal residual disease (MRD) stages.

The effect of rhodamine-123 on 3 prostate tumors from the rat.

Purpose: Rhodamine-123 (Rh-123) was found to have a specific attraction to the mitochondria of tumor cells. The destruction of rat prostate tumor cells by Rh-123 is described.

Materials And Methods: Tissue was used from rat prostate studies of Rh-123 treatment of R3327-H, PA III prostate tumor of Pollard and the autochthonous tumor in Lobund-Wistar rats.

Extracellular matrix in prostate adenocarcinoma: Changes in the glycoprotein component of the basement membrane.

This study was designed to assess the condition of the basement membrane of archival prostate carcinoma tissue when adjacent to tumor cells of varying degrees of malignancy, using a simple staining technique. Tissue was obtained from 87 patients with untreated prostate cancer. Tissue was randomly selected from four patients from each of four Gleason grades of 2, 3, 4, and 5.