Publications by authors named "Izreena Saihidi"
We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect and genus (for and ). Through the capillary effect, the results can be observed by the red band signal on the test and control lines within 5 min.
View Article and Find Full Text PDFLeptospirosis infections among hospital patients, Sarawak, Malaysia.
Trop Dis Travel Med Vaccines
November 2021
Background: Leptospirosis diagnoses have increased in Sarawak, Malaysia in recent years.
Methods: To better understand the burden of disease and associated risk factors, we evaluated 147 patients presenting with clinical leptospirosis to local hospitals in Sarawak, Malaysia for the presence of Leptospira and associated antibodies. Sera and urine specimens collected during the acute illness phase were assessed via a commercially available rapid diagnostic test (Leptorapide, Linnodee Ltd.
Article Synopsis
- In Southeast Asia and northern Australia, infections are common but often misdiagnosed, leading to high mortality rates of up to 50%.
- A 2018 pilot study involved 100 patients with infection symptoms at Kapit Hospital in Malaysia, using three diagnostic methods—including PCR and rapid testing—to detect infections.
- The study found that while the individual diagnostic tests had low sensitivity (25-44%) compared to the gold standard of bacterial culture, they demonstrated high specificity (93-98%), indicating a need for more sensitive diagnostics for better disease detection.