[The incidence of infection in tumor and eye fluid system, and specific humoral immunity to herpes viruses in patients with uveal melanoma].

Introduction: Studies aimed at a direct research of human herpes viruses (HHVs) in the tumor material and eye media have not been carried out so far. Research goal to establish the frequency of detection HHVs DNA in the biomaterial of the eye and blood and to assess the specific humoral immunity to the causative agents of herpes virus infections in patients with uveal melanoma.

Materials And Methods: 38 patients with the uveal tract tumor were examined for the presence of DNA of HHV types 1 and 2 (HSV-1, 2), Cytomegalovirus (CMV), Varicella Zoster virus (VZV), EpsteinBarr virus (EBV) and herpes viruses 6 and 8 types (HHV-6, HHV-8) in tumor tissue, vitreous body, aqueous humour and blood plasma by real-time polymerase chain reaction; blood serum was studied by enzyme-linked immunosorbent assay (ELISA) for IgG and IgM antibodies to HHVs.

[Hereditary Cushing's syndrome caused by primary bilateral macronodular adrenal hyperplasia due to ARMC5 mutation with concomitant primary hyperparathyroidism: the first known case in Russia].

Primary bilateral macronodular adrenal hyperplasia (PBMAH), a genetically heterogeneous disease, is a rare cause of Cushing's syndrome. Until recently, few cases were attributed to mutations in known genes. However, in 2013, ARMC5, a newly discovered tumor suppressor gene, was identified.

[Change in levels of plasmin and its inhibitors in blood of patients with dyscirculatory encephalopathy before and after the treatment].

The aim of the investigation was to estimate the serum concentrations of the major proteinase plasmin (PL) and some inhibitors of proteinases, such as alpha 2-macroglobulin (MG), alpha 1-antitrypsin (AT), and the specific complexes plasmin-macroglobulin (PL-MG) in order to study their role in the pathogenesis of dyscirculatory encephalopathy. All the above indices were examined in the sera of 35 patients with dyscirculatory encephalopathy (a study group) and 38 apparently healthy individuals (a control group). The authors determined the levels of PL, MG, and AT by the rocket immunoelectrophoresis technique using monospecific antisera against these proteins and those of specific PL-MG complexes by enzyme immunoassay.

Comparative pathomorphological study of contractile myocardium under conditions of increased left and right ventricular afterload.

Structural changes in the myocardium under conditions of increased left and right ventricular afterload were studied using polarization microscopy and histological, histochemical, and stereological methods. Increased afterload not complicated by heart failure was characterized by low number of damaged cardiomyocytes (3.3-6.

[Histochemical characteristics of adrenergic myocardial and adrenal fibers in experimental narrowing of the aorta and pulmonary artery].

Ligation of the aorta or the pulmonary trunk was the method of experimental overloading of heart ventricles. Histochemical studies indicated activation of mediator chain of the sympatho-adrenal system. Catecholamines content in the nervous plexus of the overloaded ventricle did not change but there was an increase of their content in the medullary layer of the adrenals.

Sympathoadrenal system under conditions of increased left and right ventricular afterload.

Adrenergic plexuses in the myocardium and adrenal medulla were studied histochemically under conditions of increased left or right ventricular afterload. Under conditions of high afterload not accompanied by heart failure the density of sympathetic myocardial innervation remained unchanged in the loaded ventricle, but increased in the intact ventricle. Comparison of the state of the sympathoadrenal system under conditions of increased afterload complicated or uncomplicated by heart failure revealed common prognostically unfavorable changes: sharp decrease in the density of adrenergic nerve plexuses in the ventricular myocardium and activation of adrenal chromaffin cells.

[Cytogenetic effect of iodized radiocontrast media in human blood lymphocytes].

Triombrast (60%) iodamide-380, free verografin and verografin incorporated in liposomes in commonly used amounts produce no significant cytogenetic effect on peripheral blood lymphocytes. An increase in the concentration of these drugs results in a decrease in the lymphocytic mitotic index and structural chromosomal disorders which are marked at the most in a 10- and 20-fold increase in a clinical dose.

[Effect of cell respiration inhibitors on the formation of structural mutations in human lymphocytes irradiated at different stages of the mitotic cycle].

Human lymphocytes were irradiated by 60Co gamma-rays after 0, 10, 20, 35, 45, 48 and 49.5 h of incubation. Immediately after irradiation sodium cyanide, sodium fluoride or monoiodoacetic acid was given for 2.

[Dependence of the yield of chromosome aberrations on the dosage in irradiating human peripheral blood lymphocytes with monoenergetic neutrons with 2, 4 and 6 MeV energies].

A study was made of the dose-dependence of the yield of chromosome aberrations in human lymphocyte culture irradiated at the G0 stage with monoenergetic neutrons of 2, 4 and 6 MeV. The dose dependence was found to be linear for all types of aberrations. The RBE of neutrons under study increased with the decrease in their energy.

[Formation of chromosome aberrations in human lymphocytes to the action of 5-fluorodeoxyuridine used separately and in combination with gamma irradiation: changes in the effect in the course of the mitotic cycle].

Human lymphocytes were treated after different times of incubation, either by 60Co gamma-rays (1 Gy) followed by 5-fluorodeoxyuridine (FUdR, 2.10-7 M during 2,5 h) or by radiation and FUdR, separately. Chromosomal aberrations were studied after 51 h of incubation.