Two new RHD alleles with deletions spanning multiple exons.

Background: The most common large-deletion RHD allele (RHD*01N.01) includes the entire coding sequence, intervening regions and untranslated regions. The rest of large-deletion RHD alleles reported to-date consist of single-exon deletions, such as RHD*01N.

RH genotyping by nonspecific quantitative next-generation sequencing.

Background: Conventional sequencing uses gene-specific primers to determine the location of RH variants and permits a qualitative assessment of zygosity. Whole-genome and whole-exome sequencing determine the genetic location of variants and enable a quantitative assessment of zygosity. Nonspecific sequencing uses RH-consensus primers to detect variants and sequencing-read ratios to quantify their copy number.

Performance evaluation study of ID RHD XT, a new genotyping assay for the detection of high-prevalence RhD negative and weak D types.

Background And Objectives: Routine serologic D typing does not distinguish between weak D subtypes and partial D phenotypes. The goal of this study was to validate the performance of the ID RHD XT genotyping assay.

Material And Methods: Previously serotyped samples for D antigen (n = 1000; 16% weak D serotyped donors) were analysed.

Performance evaluation study of ID CORE XT, a high throughput blood group genotyping platform.

Background: Traditionally, red blood cell antigens have been identified using serological methods, but recent advances in molecular biology have made the implementation of methods for genetic testing of most blood group antigens possible. The goal of this study was to validate the performance of the ID CORE XT blood group typing assay.

Materials And Methods: One thousand independent samples from donors, patients and neonates were collected from three research institutes in Spain and the Netherlands.