Dietary risk assessment of children and adults consuming fruit and vegetables with multiple pesticide residues.

Fruit and vegetables are a basic component of the human diet. European pesticide monitoring data indicated recently that in one sample multiple residues were detected which might be a public concern. Thus, the challenge of the present study was to assess the potential dietary exposure of the most critical EU children and adults subpopulations consuming fruit and vegetables.

Comprehensive toxicological multi-year study on pesticides in apples: Control, trends and dietary risk assessment.

Apples are one of the most widely consumed raw fruit worldwide. Due to multiple sprayings during the growing season, they can contain pesticide residues affecting quality of food and human health. The novelty of the present study was a comprehensive multi-year control of a broad spectrum of pesticides in apples (2006-2022) and dietary risk assessment with changing toxicological values: ADI/ARfD/MRL.

Dissipation, processing factors and dietary risk assessment of the bioinsecticide abamectin in herbal plants belonging to Lamiaceae family from open field to herbal tea infusion.

Abamectin, the mixture of avermectin B1a and B1b, is widely used as a bioinsecticide and is an alternative to chemical pest control from insects. To our knowledge, its behaviour is not fully recognized, especially in herbs. Thus, the objective of this study was to investigate the environmental fate of abamectin in herbal plants belonging to the Lamiaceae family, its dissipation in open field studies laboratory processing treatments and dietary risk assessment.

Removal of triazole and pyrethroid pesticides from wheat grain by water treatment and ultrasound-supported processes.

A simple way to reduce pesticides in cereal grains is to use washing methods. The challenge of this study was to evaluate the effectiveness of reduction of 3 triazole fungicides (difenoconazole, tebuconazole, tetraconazole) and 3 pyrethroid insecticides (beta-cyfluthrin, cypermethrin, deltamethrin) commonly used in wheat protection. Four different pre-washing methods (hot and cold water washing, twice water, and ultrasound-supported washing) were evaluated.

Impact of technological processes on tebuconazole reduction in selected cereal species and the primary cereal product, and dietary exposure assessment.

Contamination of cereals with tebuconazole (TEB) can affect the dietary risk assessment. This study investigates, for the first time, how mechanical, thermal, physical-chemical, and biochemical processes affect the TEB level in wheat, rye, and barley. The biochemical process of malting was the most effective for tebuconazole reduction (by 86%) in cereals.

A global study of pesticides in bees: QuEChERS as a sample preparation methodology for their analysis - Critical review and perspective.

To this day, it remains unknown what the cause of decline of honey bee populations is and how to prevent this phenomenon efficiently. Poisonings with pesticides are assumed to be among the main causes for the decline of the honey bee population. Despite the significant progress observed in analytics over recent years, research aimed at improving methods applied in diagnostics of bee poisoning is still in progress.

Impact of broad-spectrum pesticides used in the agricultural and forestry sector on the pesticide profile in wild boar, roe deer and deer and risk assessment for venison consumers.

The present work is the first extensive study of large-scale pesticides research in wild animals. The investigation covered three game species: wild boar (n = 42), roe deer (n = 79) and deer (n = 15) collected from north-eastern Poland. To characterize the 480 pesticides in muscle samples, LC-GC-MS/MS techniques were used.

Exposure of wild boars (Sus scrofa L) to neonicotinoid insecticides.

The aim was to determine, for the first time, concentrations of 7 neonicotinoids (NEOs) and 5 metabolites in Sus scrofa from hunting areas in north-eastern Poland and assess the risk to consumers eating boar meat. 42 wild boar muscle samples were collected over a one-year period. The concentrations of 12 NEOs were determined by a fully validated LC-ESI-MS/MS protocol based on ultrasonic, freezing and cleanup EMR-lipid sample preparation.

Development of precise micro analytical tool to identify potential insecticide hazards to bees in guttation fluid using LC-ESI-MS/MS.

This paper illustrates the development of a miniaturized and precise analytical tool for biomonitoring of honey bee exposure to insecticides. This is the first work describing an analytical method for determination of very low concentrations of a wide range of insecticides in maize guttation fluid. Seed treatment with systemic insecticides or their foliar application causes the accumulation of compounds in the guttation liquid, which consists of excess water and compounds removed by plants and is a source of water for bees.

Comprehensive analysis of insecticides in melliferous weeds and agricultural crops using a modified QuEChERS/LC-MS/MS protocol and of their potential risk to honey bees (Apis mellifera L.).

The risk of exposure of honey bees to the presence of insecticides in melliferous plants is higher than previously reported. Therefore, monitoring insecticide residues in these plants is of great importance to honey bee safety. A novelty of the present research was the development of an innovative procedure for determination of residues of 142 insecticides in weeds and agricultural crops among melliferous plants.

Dissipation of S-metolachlor in plant and soil and effect on enzymatic activities.

The present study aimed at evaluating the dissipation of S-metolachlor (S-MET) at three doses in maize growing on diverse physico-chemical properties of soil. The effect of herbicide on dehydrogenase (DHA) and acid phosphatase (ACP) activity was estimated. A modified QuEChERS method using LC-MS/MS has been developed.

Evolution of novel sorbents for effective clean-up of honeybee matrix in highly toxic insecticide LC/MS/MS analysis.

Highly toxic insecticides (HTIs) belonging to different chemical groups are dangerous to pollinating organisms, even in sublethal doses. An important objective of this study was to develop a method to determine over fifty HTIs at very low concentrations in the bee matrix. The novelty of this research involved obtaining extract completely free from beewaxes, lipids and proteins using EMR-lipid (enhanced matrix removal-lipid), chitin and Z-Sep+ (zirconium oxide and C18 dual-bonded to silica) as clean-up sorbents.

Behaviour of mesotrione in maize and soil system and its influence on soil dehydrogenase activity.

The aim of this study was to investigate the dissipation of mesotrione and effect on dehydrogenase activity (DHA) in maize and soil system. The paper for the first time describes behaviour of this herbicide applied at various doses (separately or in mixture with other herbicide) in acidic and alkaline environment. The experiments were conducted using the method randomized blocks in four repetition cycles.

Rapid determination of acid herbicides in soil by liquid chromatography with tandem mass spectrometric detection based on dispersive solid phase extraction.

This study determined twenty six the highly sensitive phenoxy, pyridines, aliphatic and aromatic acid compounds in soil with a liquid chromatography tandem mass spectrometry. The samples were prepared by modified quick, easy, cheap, effective, rugged and safe (QuEChERS) analytical procedure in solid samples. Herbicides extraction effectiveness was evaluated at three different spiking levels (0.

Dissipation of six fungicides in greenhouse-grown tomatoes with processing and health risk.

Greenhouse studies were conducted to evaluate the dissipation rate kinetics and estimate the behavior of selected pesticides after washing, peeling, simmering, and canning of tomato expressed as processing factor (PF). Two varieties (Marissa and Harzfeuer) were treated by six fungicides: azoxystrobin, boscalid, chlorothalonil, cyprodinil, fludioxonil, and pyraclostrobin at single and double dose and risk assessment defined as hazard quotient was performed. The QuEChERS method was used for sample preparation followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

Multi-residue methods for the determination of over four hundred pesticides in solid and liquid high sucrose content matrices by tandem mass spectrometry coupled with gas and liquid chromatograph.

For the first time three methods: matrix solid phase dispersion (MSPD), original and modified QuEChERS, with and without clean up step were studied in order to evaluate the extraction efficiency of various classes of pesticides from solid and liquid high sucrose content matrices. Determinations over four hundred pesticides were performed by gas and liquid chromatography with triple quadrupole mass spectrometry (GC/LC/MS/MS) using multiple reaction monitoring. The proposed methods were validated on sugar beets and their technological product beet molasses.

Removal of 16 pesticide residues from strawberries by washing with tap and ozone water, ultrasonic cleaning and boiling.

The effects of washing with tap and ozone water, ultrasonic cleaning and boiling on 16 pesticide (ten fungicides and six insecticides) residue levels in raw strawberries were investigated at different processing times (1, 2 and 5 min). An analysis of these pesticides was conducted using gas chromatography with nitrogen-phosphorous and electron capture detection (GC-NPD/ECD). The processing factor (PF) for each pesticide in each processing technique was determined.

The evaluation of a fast and simple pesticide multiresidue method in various herbs by gas chromatography.

In this study two analytical methods, one based on matrix solid phase dispersion (MSPD) and the other on liquid-solid extraction (LSE), coupled with gas chromatography, were evaluated and used to determine the presence of 163 pesticides (6 acaricides, 62 fungicides, 18 herbicides and 77 insecticides) in various herbs. Both methods were optimized considering different parameters (sample to sorbent mass ratio, extracting solvent, sorbents for clean-up step, etc.).