A novel typing method for Listeria monocytogenes using high-resolution melting analysis (HRMA) of tandem repeat regions.

Listeria monocytogenes, which is responsible for causing food poisoning known as listeriosis, infects humans and animals. Widely distributed in the environment, this bacterium is known to contaminate food products after being transmitted to factories via raw materials. To minimize the contamination of products by food pathogens, it is critical to identify and eliminate factory entry routes and pathways for the causative bacteria.

A rapid typing method for Listeria monocytogenes based on high-throughput multilocus sequence typing (Hi-MLST).

Listeria monocytogenes infects humans via food products, causing listeriosis. Consequently, food companies pay meticulous attention to the risk of contamination of their products by this bacterium. While fragment analysis methods such as pulsed-field gel electrophoresis (PFGE) are used to trace the sources of contamination for this bacterium, some drawbacks have been identified, namely the complexity of the methods and the difficulty of making data comparisons.

Seroprevalence of Helicobacter pylori infection in Nepal: low prevalence in an isolated rural village.

Objective: To determine the seroprevalence of Helicobacter pylori infection in Nepal.

Design: H. pylori infection was identified using a specific and sensitive enzyme-linked immunosorbent assay for anti-H.

[Management of the impalpable testis; the value of laparoscopy for subsequent treatment].

During a 5-year period between April 1984 and March 1989, we were unable to palpate 62 testes (56 child patients) at the time of diagnosis. An average of 10 months later, 51 testes (48 patients) still remained impalpable preoperatively. Of the two imaging techniques, computed tomography and ultrasonography, the former is rather superior to the latter for verifying the existence of the impalpable testis.

[Intrascrotal sclerosing lipogranuloma: a case report].

We report a case of genital sclerosing lipogranuloma, which lacks any causative agents or episode of genital trauma. The patient was a single male of 27 years old. Open biopsy revealed it lipogranuloma with no evidence of malignancy.

Promotion of lung tumor colonization in mice by the synthetic thrombin inhibitor (no. 805) and its reversal by leech salivary gland extracts.

The role of anticoagulation per se in the reduction of experimental or spontaneous metastasis still remains to be determined, as shown by the conflicting results reported by the literature using different conventional anticoagulants. A new compound has been synthesized (compound no. 805) which prolongs or suppresses coagulation via specific inhibition of thrombin and its possible use in a model of experimental metastasis to clarify the role of anticoagulants in tumor spread was investigated.

Functional recovery of the bladder in patients with spinal cord injury--prognosticating programs of an aseptic intermittent catheterization.

Ninety patients with spinal cord injury were managed by aseptic intermittent catheterization program I (preventing the over-distension of the bladder) and program II (allowing overdistension) in which recovery of bladder function and the clinical effects were comparatively investigated. Using program I, cases with positive BCR restored the automaticity of the bladder on average 8 weeks after injury. When urinary incontinence first occurs during aseptic intermittent catheterization, urinary training may be commenced since the bladder has then recovered from spinal shock.

Leech salivary gland extract from Haementeria officinalis, a potent inhibitor of cyclophosphamide- and radiation-induced artificial metastasis enhancement.

Studies were designed to determine whether salivary gland extract (SGE) from the leech Haementeria officinalis could inhibit enhancement of lung tumor colonization induced by pretreatment of mice with cyclophosphamide (CY) or local thoracic irradiation (LTI). Tumor nodules in the lung were generated by i.v.

Effect of antifibrinolysis treatment on human cancer in nude mice.

The effect of tranexamic acid on the growth of three human tumor cell lines (lung squamous cell carcinoma QG-56, lung adenocarcinoma PC-12 and ovarian carcinoma OC-1) was investigated in vitro and in vivo in nude mice. Tranexamic acid exhibited no direct cytotoxicity in vitro, but it inhibited the growth of all the three cell lines as tumors in nude mice. Prominent deposition of fibrin or fibrinogen around tumor cells at the advancing border were observed only in the tumors of treated animals.

Effect of fibrinolysis inhibitor and chemotherapeutics on the growth of human cancers transplanted into nude mice and in tissue culture.

Effects of tranexamic acid (AMCHA) alone or together with anticancer drug on the growth of human cancers transplanted into nude mice and in tissue culture were investigated. AMCHA showed no effects on cancer cells in vitro. Fibrin deposition and host stromal reaction at the advancing border of the tumor tended to increase after AMCHA administration.

Heterotransplantation of human urinary bladder cancers in nude mice.

Thirty-one human urinary bladder cancers were transplanted subcutaneously into congenitally athymic nude mice. Subcutaneous growth was obtained in eight at the initial transplantation. Only one of these eight successful transplants, which was derived from squamous cell carcinoma, has been serially transplanted successfully and was characterized by high thromboplastic and low fibrinolytic activities which were virtually constant throughout the serial passages.