[An approach to a methodology of scientific research for assistant-students].

Background: This work is presented from a "problematic" perspective in the attempt to establish a dialogic relationship between the educator and the student-subject, mediated by the object of knowledge. It is oriented to the integral education of the helping students departing from a closer approach to the scientific research. This work was carried out by a teacher and two hired students.

Interferon gamma production by PAS-positive T lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions.

Unlabelled: In the blood of chagasic patients, a high number of T-lymphocytes producers of a PA.S.-positive substance was found, more numerous in chagasics with abnormal electrocardiogram.

[An experience in the use of NTIC (New Technology for Information and Communication) in the teaching process of the immune system].

Introduction: Not very often an integrated approach is applied in knowledge areas as Immune System, instead, the contents are usually a sort of "atomization" that prevents from having a holistic vision of the set as a whole. At the moment, our university students usually incorporate the Internet in their study work, similarly, the teachers can resort to this tool to "widen" the boundaries of the real classes. Perhaps, to achieve a deeper study level in this knowledge field, the use of NTIC may result an appropriate resource for complementing and supporting the current conventional techniques.

[Gangliosides in vitro effects on lymphocytes from patients with chronic Chagas disease stimulated with mitogens or heart or brain antigens].

In human Chagas'disease previous work has shown the occurrence of a T-lymphocyte CD4-positive population (a high producer of PAS-positive glycoproteins) with evidence suggesting a role in the formation of damages to the myocardium and neural structures in chagasic heart disease (ChHD). Other workers have taken such facts into consideration and have employed gangliosides (biological substances with neurotrophic and immunomodulatory properties) in chagasics with chronic cardiomyopathy and disautonomic signs, obtaining an Improvement in functional signs and a decrease in the number of PAS+ lymphocytes. In the present work we have studied the effect of mixed gangliosides (Cronassial on cell cultures of total leukocytes, or on mononuclear cells prepared through Ficoll-Hypaque.