Markers of Mitochondrial Injury and Neurological Outcomes of Comatose Patients after Cardiac Arrest.

: Most patients who are successfully resuscitated from cardiac arrest remain comatose, and only half regain consciousness 72 h after the arrest. Neuroprognostication methods can be complex and even inconclusive. As mitochondrial components have been identified as markers of post-cardiac-arrest injury and associated with survival, we aimed to investigate cytochrome c and mtDNA in comatose patients after cardiac arrest to compare neurological outcomes and to evaluate the markers' neuroprognostic value.

Platelet activation and blood extracellular vesicles: The influence of venepuncture and short blood storage.

Unlabelled: Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs.

Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay.

Heparin-induced thrombocytopenia: effect of heat inactivation of patient's serum on functional flow cytometric assay results.

Background: Heat inactivation of a patient's sample is not systematically performed in the diagnostics of heparin-induced thrombocytopenia (HIT). Some authors recommend that the patient's sample is heat-inactivated to avoid the effect of thrombin on platelet activation in a functional assay. Others do not find this additional step essential or even advise against it.

Haemato-biochemical profile and acid-base status of Croatian spotted goats of different ages.

The aim of the present research was to determine the haemato-biochemical profile and blood acid-base status of Croatian spotted goats in a traditional Mediterranean production system. The 60 non-gravid female Croatian spotted goats of different ages were included in the research. They were divided into four groups of 15 goats according to age: group I -  year old; group II - 2-3 years; group III - 3-6 years; and group IV - 7-10 years.