Liposome-encapsulated aprotinin biodistribution in mice: Side-by-side comparison with free drug formulation.

Injuries of the respiratory system caused by viral infections (e.g., by influenza virus, respiratory syncytial virus, metapneumovirus, or coronavirus) can lead to long-term complications or even life-threatening conditions.

Aprotinin-Drug against Respiratory Diseases.

Aprotinin (APR) was discovered in 1930. APR is an effective pan-protease inhibitor, a typical "magic shotgun". Until 2007, APR was widely used as an antithrombotic and anti-inflammatory drug in cardiac and noncardiac surgeries for reduction of bleeding and thus limiting the need for blood transfusion.

The Efficacy of Aprotinin Combinations with Selected Antiviral Drugs in Mouse Models of Influenza Pneumonia and Coronavirus Infection Caused by SARS-CoV-2.

The efficacy of aprotinin combinations with selected antiviral-drugs treatment of influenza virus and coronavirus (SARS-CoV-2) infection was studied in mice models of influenza pneumonia and COVID-19. The high efficacy of the combinations in reducing virus titer in lungs and body weight loss and in increasing the survival rate were demonstrated. This preclinical study can be considered a confirmatory step before introducing the combinations into clinical assessment.

Synthesis and downconversion photoluminescence of Erbium-doped chalcohalide glasses of AgCl(I)-GaS-LaS systems.

Erbium-doped chalcohalide glasses of the ()-- systems were synthesized by using the melting-cooling method. The introduction of a halide component leads to a decrease in the absorption coefficient, and the addition of erbium leads to the emergence of narrow absorption bands associated with transitions in the f-shell of ions. Five PL bands were detected in erbium-doped samples when excited by a laser with a 532 nm wavelength, the most intense of which at 980 nm and 1540 nm are promising for use in optical amplifiers and fiber optic networks.

[THE RESULTS OF SCREENING OF DONATED BLOOD IN THE UKRAINE THE PRESENCE OF MARKERS HEMOTRANSMISYVNYH INFECTIONS IN 2010-2012 YEARS].

Prevalence level of HIV markers among blood and blood coMponent donors in European region of WHO increased from 8.3 to 10.3 positive cases per: 100,000 donations diring 2001-2006.

[The hygiene of clothing for children and adolescents with obesity on the basis of characteristics of heat exchange].

Metabolic syndrome in pediatric and adolescent populations is particularly alarming for its frequent occurrence. Comparative analysis of dimensional typology and percentile values shows exceeding typical dimensional signs. Choosing appropriate clothes feel comfortable for such children is limited.

[Efficiency of aerosol therapy in rehabilitation programs for patients with occupational broncho-pulmonary diseases].

The authors demonstrated efficiency of aerosol therapy using "Dovolenskaya" mineral water in treating patients with occupational pulmonary diseases. Results are improved bronchial drainage, normal pro- and antioxidant status, lower inflammatory activity in patients with pneumoconiosis and occupational dust bronchitis.

[The late results of electrical cardioversion in patients with a chronic form of atrial fibrillation].

The paper presents results of study of the electric cardioversion (ECV) long-term effects in 216 patients with a chronic form of cardiac fibrillation (CF). The CF relapses were found to occur in 45.4% of cases, with the 43.

[Dependence of epidermal growth factor activation on its affinity and oligomerization].

Epidermal growth factor receptor (EGF-R) oligomerization has been followed on A-431 cells using covalent labeling by 125I-EGF and EGF-dependent autophosphorylation of receptor-kinase. High molecular weight complexes corresponding to monomeric, dimeric, and trimeric forms of EGF-R are detected. The process of oligomerization occurs effectively at 37 degrees C while at 4 degrees C no oligomer formation is detected.

[Bile acid levels in the serum and the state of liver blood flow in patients with rheumatic mitral heart disease].

The total and fractional content of blood bile acids (BA) was studied by the method of thin-layer chromatography and rheohepatography was performed in 87 patients with rheumatic mitral disease (mitral stenosis and associated mitral heart disease with prevalence of stenosis). There was a direct dependence between the degree of clinical and rheohepatographic signs of disorders of the hemodynamics and increase of the level of serum BA, a more pronounced therapeutic effect with normalization of BA, concentration in the blood after complex treatment using hepatotropic drugs than with use of traditional drugs (non-steroid antiinflammatory, cardiac glycosides, diuretics and others.).

[The effect of epidermal growth factor on protein phosphorylation in normal and transformed hepatocytes].

Qualitative differences in the content of tyrosine-phosphorylated proteins in normal and transformed hepatocytes have been found using the method of two-dimensional electrophoresis. Epidermal growth factor (EGF) has induced quantitative changes in the spectra of phosphotyrosine-containing proteins in normal cells and qualitative changes in the transformed ones. Results of immunoprecipitation with antibodies against phosphotyrosine permit revealing a protein with Mm 50 kDa which is subjected to EGF-dependent tyrosine-phosphorylation in normal hepatocytes.

[Changes in the contents of epidermal growth factor and EGF-like polypeptides in the liver tissue of rats in N-nitrosodiethylamine-induced hepatocarcinogenesis].

It has been determined that concentration of EGF-like substances in the liver of rats with N-diethylnitrosamine-induced hepatocarcinogenesis increases and reaches its maximum in tumours (50-150 ng/mg protein). In the regenerating liver the amount of these peptides does not exceed 10 ng/mg protein. High pressure gel-filtration of appropriate extracts has revealed EGF-competing substances with m.

[Changes in the epidermal growth factor receptors in the liver cells of rats in N-nitrosodiethylamine-induced hepatic carcinogenesis].

A sharp decrease in the number of epidermal growth factor receptors (EGF-R) in the rat liver plasma membranes had been found at different stages of diethylnitrosamine-induced carcinogenesis. The complete loss of high-affinity binding sites for EGF did not prevent EGF-dependent autophosphorylation of EGF-R. Hepatocytes from the rat liver tumors in the primary culture had two classes of EGF-R: high and low affinity ones, though their number had been twice less than in the normal hepatocytes.

[Increase in the level of polypeptide growth factors and their receptors in induced carcinogenesis and regeneration of the intestinal mucosa in rats].

It has been established for the first time that in extracts of the regenerating and preneoplastic (4 months after the beginning of carcinogen introduction) intestine as well as in the peritumor tissue the content of EPR-similar polypeptides and insulin raises, whereas in tumours it remains not high. Proteins with molecular weight greater than 120 kD able to compete with 125I-EPR for the binding with the receptors of EPR and being, evidently, the precursors of EPP are found in case of carcinogenesis. Besides, the content of insulin receptors rises, this process being most typical of the large intestine.

[Change in the number of epidermal growth factor receptors of hepatocytes during liver regeneration in rats].

The number of epidermal growth factor (EGF) receptors was determined for rat's hepatocytes under condition of their regeneratory proliferation, following a partial hepatectomy. In the normal hepatocytes two classes of EGF-receptors are distinguished, with high and low affinity, respectively. As early as 2 hours after the partial hepatectomy the total number of EGF-receptors sharply decreases, those with high affinity being not determined at all.

[The production of epidermal growth factor and polypeptides similar to it in the intestinal mucosa of rats in 1,2-dimethylhydrazine-induced carcinogenesis and compensatory regeneration].

The production of EGF and EGF-like polypeptides in the normal intestinal mucosa and during 1,2-dimethylhydrazine (DMH)-induced carcinogenesis and postresection regeneration was studied in albino rats using chromatographic separation of acid-ethanol extracts. Fractions after gel filtration on Biogel P-60 with subsequent reverse-phase high performance liquid chromatography in acetonitrile gradient were tested in radioreceptor assay for competition with EGF. It has been established that intestinal tumours induced by DMH and regenerating intestinal mucosa have amplified production of EGF--alpha-TGF and related proteins of high molecular weight (approx.

[Effect of the epidermal growth factor on 1,2-dimethylhydrazine-induced carcinogenesis in the rat intestinal mucosa].

The radioimmunological and radioreceptor methods have been used to show that sialadenectomy leads to the stable decrease of the epidermal growth factor (EGF) concentration in saliva and blood serum. The mean number of colon tumours per rat was significantly lower among the rats which had been sialadenectomized before injections of the carcinogen, than in the control. But a sharp stimulation of carcinogenesis in the duodenal mucosa was observed after sialadenectomy.

[Proliferation of the intestinal epithelium and of the regenerating liver with a deficiency of the epidermal growth factor].

To determine the role of the epidermal growth factor (EGF) in the regenerative stimulation of intestinal epithelium and hepatocyte proliferation after partial resection of these organs the labeling index of the intestine and liver in sialadenectomized rats was studied. EGF concentration in the saliva and serum was determined using radioimmunoassay. The decrease in EGF concentration after the removal of submandibular salivary glands was shown to slower hepatocytes entering the mitotic cycle and to inhibit the activity of enterocyte proliferation in the small intestine.

[Characteristics of the epidermal growth factor receptors in the epithelium of the gastrointestinal tract of rats and in intestinal tumors induced by 1,2-dimethylhydrazine].

Using the Scatchard analysis of 125J-EGF binding it was shown that plasma membranes of gastric and small intestinal epithelial cells contain approximately 20 times less EGF-receptors (EGF-R) than liver cells. Investigation of the phosphorylation activity of EGF-R-kinase was performed in vitro on plasma membranes from intestine, intestinal tumours and liver cells. The main protein phosphorylated at tyrosine in the tumours was p34 but the intensity of EGF-R autophosphorylation and its total protein-tyrosine kinase activity were reduced (as compared with small-intestinal and liver cell membranes).

[Interaction of growth factors and oncogenes in the tumorous transformation of cells].

Different aspects of the interaction of polypeptide growth factors with their receptors are reviewed. The problem of structural and functional interactions of the normal and transforming growth factors as well as the protein products of oncogenes during transmittance of a mitogenic signal and the proliferation regulation of normal and tumour cells is discussed.