Nanoluciferase-Based Method for Detecting Gene Expression in .

Genetic reporters such as the green fluorescent protein (GFP) can facilitate measurement of promoter activity and gene expression. However, animal autofluorescence limits the sensitivity of GFP and other fluorescent reporters in whole-animal settings like in the nematode Here, we present a highly sensitive Nanoluciferase (NanoLuc)-based method in a multiwell format to detect constitutive and inducible gene expression in We optimize detection of bioluminescent signals from NanoLuc in and show that it can be detected at 400,000-fold over background in a population of 100 animals expressing intestinal NanoLuc driven by the promoter. We can reliably detect signal in single ::-expressing worms from all developmental stages.