Publications by authors named "Ivana Okic-Djordjevic"
Purpose Of Review: Along with a strong impact on skeletal integrity, bone marrow adipose tissue (BMAT) is an important modulator of the adult hematopoietic system. This review will summarize the current knowledge on the causal relationship between bone marrow (BM) adipogenesis and the development and progression of hematologic malignancies.
Recent Findings: BM adipocytes (BMAds) support a number of processes promoting oncogenesis, including the evolution of clonal hematopoiesis, malignant cell survival, proliferation, angiogenesis, and chemoresistance.
Article Synopsis
- Adipose tissue (AT) exists in different locations in the body, including subcutaneous, visceral, and bone marrow regions, and varies in insulin sensitivity, fat breakdown, and response to diseases.
- Despite advancements in understanding the origins of these fat cells, studying their stem cell environments remains challenging.
- The review highlights how AT influences tumor development by affecting the behavior of both healthy and cancerous stem cells and discusses the changes in mature fat cells prompted by tumors.
Objectives: Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull.
Materials And Methods: Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33.
Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation.
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- Lipopolysaccharide (LPS) from Escherichia coli affects the function of human periodontal ligament stem cells (PDLSCs) by inhibiting osteogenesis and promoting chondrogenesis and adipogenesis, while not impacting their viability or proliferation.
- LPS treatment enhances the myofibroblast-like properties of PDLSCs, increasing their contractility and expression of molecules such as TGF-β and fibronectin, while also altering inflammatory marker expressions like COX-2 and IL-6.
- The study indicates that LPS activates the ERK1,2 signaling pathway, contributing to the differentiation changes and alterations in the immunomodulatory functions of PDLSCs.
Article Synopsis
- - The tumor microenvironment (TME) significantly influences tumor growth and can lead to treatment resistance and disease relapse due to its interactions with various cells, including tumor, immune, and mesenchymal stem cells (MSCs).
- - MSCs can alter their characteristics and functions in response to signals from the TME, which often promotes tumor growth and dampens the immune response against tumors.
- - This review discusses how the inflammatory TME modifies MSCs' roles in tumor development and explores the potential of using MSCs as targeted delivery systems for cancer treatment.
Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-γ (IFN-γ) and/or tumor necrosis factor-α (TNF-α) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro.
View Article and Find Full Text PDFAims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins.
Main Methods: Immunophenotyping, proliferation and differentiation assays were performed.
Aim: To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011).
Methods: Animals were irradiated by a Cobalt gamma source at 6.7 Gy.
Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients.
View Article and Find Full Text PDFIntroduction: Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent.
Objective: The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton's Jelly (UC-MSCs).
Methods: The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential.