Inclusion complexation with β-cyclodextrin derivatives alters photodynamic activity and biodistribution of meta-tetra(hydroxyphenyl)chlorin.

Application of meta-tetra(hydroxyphenyl)chorin (mTHPC) one of the most effective photosensitizer (PS) in photodynamic therapy of solid tumors encounters several complications resulting from its insolubility in aqueous medium. To improve its solubility and pharmacokinetic properties, two modified β-cyclodextrins (β-CDs) methyl-β-cyclodextrin (M-β-CD) and 2-hydroxypropyl-β-cyclodextrin (Hp-β-CD) were proposed. The aim of this work was to evaluate the effect of β-CDs on mTHPC behavior at various stages of its distribution in vitro and in vivo.

Estimation of blood microcirculation in integuments by non-invasive speckle-optical method under the photodynamic action.

In studies on animals (rats, rabbits) an experimental technique for estimation of microhemocirculation parameters of tissue under treatment has been developed. This technique has been used to compare the changes of speckle-optical parameters of skin microhemodynamics in laser-irradiated and light-isolated areas in the course of photodynamic therapy. Strong correlation between efficiency of tissue response and injection-photoirradiation time interval has been established.

Investigation of Foscan interactions with plasma proteins.

The present study investigates the interaction of the second generation photosensitizer Foscan with plasma albumin and lipoproteins. Spectroscopic studies indicated the presence of monomeric and aggregated Foscan species upon addition to plasma protein solutions. Kinetics of Foscan disaggregation in albumin-enriched solutions were very sensitive to the protein concentration and incubation temperature.