Identification of L-asparaginases from strains with competitive activity and immunogenic profiles: a bioinformatic approach.

The enzyme L-asparaginase from is a therapeutic enzyme that has been a cornerstone in the clinical treatment of acute lymphoblastic leukemia for the last decades. However, treatment effectiveness is limited by the highly immunogenic nature of the protein and its cross-reactivity towards L-glutamine. In this work, a bioinformatic approach was used to identify, select and computationally characterize L-asparaginases from through sequence-based screening analyses, immunoinformatics, homology modeling, and molecular docking studies.