Clock and riboswitch control of in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis.

Metabolic homeostasis is regulated by enzyme activities, but the importance of regulating their corresponding coenzyme levels is unexplored. The organic coenzyme thiamine diphosphate (TDP) is suggested to be supplied as needed and controlled by a riboswitch-sensing mechanism in plants through the circadian-regulated gene. Riboswitch disruption negatively impacts plant fitness.

The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus.

In plants, metabolic homeostasis-the driving force of growth and development-is achieved through the dynamic behavior of a network of enzymes, many of which depend on coenzymes for activity. The circadian clock is established to influence coordination of supply and demand of metabolites. Metabolic oscillations independent of the circadian clock, particularly at the subcellular level is unexplored.

Clarification of the dispensability of PDX1.2 for Arabidopsis viability using CRISPR/Cas9.

Background: PDX1.2 has recently been shown to be a regulator of vitamin B biosynthesis in plants and is implicated in biotic and abiotic stress resistance. PDX1.