Effective Isolation of Picrocrocin and Crocins from Saffron: From HPTLC to Working Standard Obtaining.

Saffron is widely cultivated and used as a spice. Recently published data on the chemical composition and pharmacological potential of saffron determine its use in pharmacy and medicine. The proposed high-performance thin-layer chromatography (HPTLC) method allows good separation of 11 analytes.

Bioactive constituents of Iris hybrida (Iridaceae): Processing effect.

Iris genus plants are a valuable source of bioactive compounds, which are an important component for pharmaceutical development. The present article shows the potential for mineral nutrition with application of magnesium sulfate, iron chelates and potassium oxide affecting the phenolic compound contents in Iris hybrida 'Tsikavynka', I. hybrida 'Tambo' and I.

Comparative analysis of apocarotenoids and phenolic constituents of Crocus sativus stigmas from 11 countries: Ecological impact.

The chemical compositions of 15 saffron samples from 11 countries (Morocco, India, Italy, Spain, Germany, Switzerland, Iran, Lithuania, Ukraine, Australia, and Azerbaijan) were evaluated. The samples were analyzed regarding the impact of environmental factors on the composition of apocarotenoids and phenolic constituents. Quantification of saffron metabolites was carried out using high-performance liquid chromatography.

Pharmacological Potential and Chemical Composition of Leaf Extracts.

L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. sp.

Effective and simple approach for colchicine determination in saffron parts.

Crocus sativus (Iridaceae) or saffron is important for its flavoring properties in the food industry and its medicinal properties in the pharmaceutical industry. Real saffron must meet the quality requirements set to FDA or ISO specifications, and in addition, must be safe and non-toxic. An "autumn crocus" or scientific name Colchicum spp.

Bio-guided bioactive profiling and HPLC-DAD fingerprinting of Ukrainian saffron (Crocus sativus stigmas): moving from correlation toward causation.

Background: Saffron or stigmas of Crocus sativus L. is one of the most valuable food products with interesting health-promoting properties. C.

Comparative Analysis of the Major Metabolites of Ukrainian Saffron Samples by HPLC.

Crocus sativus L. is one of the most valuable crops, which stigmas are used as the food supplement and herbal medicine. In Ukraine, the cultivation of C.

Simultaneous determination of amoxicillin and potassium clavulanate in combined medicinal forms: procedure transfer from HPLC to UPLC.

This article presents the results of transferring a high-performance liquid chromatography (HPLC) procedure for the assay of amoxicillin and potassium clavulanate in tablets to the ultra-performed liquid chromatography (UPLC) conditions. Since the State Pharmacopoeia of Ukraine (SPhU) does not contain the monograph for the simultaneous analysis of amoxicillin and clavulanic acid, the British Pharmacopoeia procedure was used. Parameters of the procedure were optimized to fit the UPLC and to make a better performance.

Estimation of the influence of the environmental factors on the accumulation of phytochemicals and antioxidant capacity in the ivy leaves ( L.).

This study aimed at evaluating the impact of environmental factors on the accumulation of various components in collected from different European countries. The obtained results of studied samples showed that the antioxidant capacities were in the range of 0.027 to 0.

Qualitative and Quantitative Analysis of Ukrainian Species: A Fresh Look on Their Antioxidant Content and Biological Activities.

The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian sp. (, , , and ) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3',5'-tetramethoxyisoflavone, irisolidone-7---d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds.

Effect of ecological factors on the accumulation of phenolic compounds in Iris species from Latvia, Lithuania and Ukraine.

Introduction: It is important to conduct studies on the influence of environmental factors on the accumulation of secondary metabolites in plants, as well as the cultivation of plants and harvesting of their raw material.

Objective: In this study, we examined the influence of habitat types, soil composition, climatic factors and altitude on the content of phenolic compounds in Iris species from different populations in Latvia, Lithuania and Ukraine.

Methodology: According to high-performance liquid chromatography (HPLC) analysis, 25 compounds (flavonoids, isoflavonoids, isoflavonoid glucosides, xanthones, phenolcarboxylic acids) were identified in the methanol extracts of 16 samples of Iris rhizomes.

Development and validation of a HPLC method for quantifica-tion of degradation impurities of salbutamol sulfate with following long-term stability studies in multicomponent cough syrup.

Medicines containing both a herbal extract and a synthetic substance are in high demand due to their beneficial effects and synergism. The novel combination of salbutamol sulfate and Hedera helix extracts seems to be prospective in terms of pharmacological activity. But for quality assurance, impurities of the synthetic component have to be determined and quantified.