Publications by authors named "Ivan Arenas-Sosa"
Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. , an entomopathogenic nematode (EPN), was monitored for its performance when produced in vivo, as well as its development using larvae, using the MC5-2014 strain isolated from soil samples in the municipality of Tepalcingo, Morelos, México.
View Article and Find Full Text PDFChihuahua is the largest state in Mexico. The ecosystem of this region is composed of large area of bushes, forests, and grasslands, which allows for a specific diversity of fauna; among them are interesting species of non-lethal scorpions. Most of the Chihuahuan scorpions have been previously morphologically and molecularly described; however, this manuscript could be the first to describe the composition of those venoms.
View Article and Find Full Text PDFArticle Synopsis
- Bacillus thuringiensis (Bt) toxins are known for their effectiveness against certain insects, but they are typically less effective on sap-sucking insects like aphids.
- The study focuses on the Cry toxin from Bt strain GP919, which demonstrated over 80% mortality in the aphid Myzus persicae at a concentration of 10 ng/µl, while showing no toxicity to the caterpillar Spodoptera frugiperda.
- The research identifies a specific Cry1Cb2 protein that is lethal to aphids and suggests it could be an innovative, eco-friendly method for controlling sap-sucking pests in agriculture.
A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the municipality of Tepalcingo, Morelos, in soil with a sandy loam texture and a pH of 6.4. Species determination was performed via morphological and morphometric techniques by searching for a tubular stoma with a swollen cylindrical pharyngeal body and a metacorpus in the basal part.
View Article and Find Full Text PDFArticle Synopsis
- The study explored how two antimicrobial peptides (AMPs), Pin2 and its variant Pin2[GVG], interact with different lipid membrane models representing bacterial, erythrocyte, and fungal cells to determine their effectiveness and selectivity.
- The experiments revealed that the AMPs had the strongest affinity for bacterial models, and the presence of sterols influenced the selectivity of the variant, while the wild type remained unchanged.
- Techniques like calcein liposome leakage, Dynamic Light Scattering, and molecular dynamics simulations indicated that these peptides form pores in bacterial membranes without affecting their size, leading to membrane changes such as increased viscosity and electrical resistance alterations.
The gene of the four disulfide-bridged defensin J1-1 from Capsicum was cloned into the expression vector pQE30 containing a 6His-tag as fusion protein. This construct was transfected into Origami strain of Escherichia coli and expressed after induction with isopropyl thiogalactoside (IPTG). The level of expression was 4 mg/L of culture medium, and the His-tagged recombinant defensin (HisXarJ1-1) was expressed exclusively into inclusion bodies.
View Article and Find Full Text PDFThe sublethal effects of two strains of Bacillus thuringiensis, which were virulent in vitro to Varroa destructor, were measured on Apis mellifera. The effects of five concentrations of total protein (1, 5, 25, 50 and 100μg/mL) from the EA3 and EA26.1 strains on larval and adult honey bees were evaluated for two and seven days under laboratory conditions.
View Article and Find Full Text PDFParasitic agents, such as helminths, are the most important biotic factors affecting aquaculture, and the fluke Centrocestus formosanus is considered to be highly pathogenic in various fish species. There have been efforts to control this parasite with chemical helminthicides, but these efforts have had unsuccessful results. We evaluated the anthelmintic effect of 37 strains of Bacillus thuringiensis against C.
View Article and Find Full Text PDF