[The diagnostic and prognostic significance of the degree of neutrophilic leukocyte activation in the peripheral blood of patients with odontogenic phlegmons].

Structural and morphologic changes in peripheral blood neutrophilic leukocytes were examined with the aim to diagnose and predict the clinical course of acute pyoinflammatory maxillofacial diseases. Classification of structural and morphologic signs of neutrophilic leukocyte activation levels during odontogenic pyoinflammatory diseases were studied using optic and electron microscopy. There degrees of neutrophilic leukocyte activation were distinguished: inactive, moderately active, and hyperactive.

[The assessment of the functional activity of peripheral blood neutrophils in patients with odontogenic phlegmons].

To objectively assess the status of patients with odontogenic phlegmons their peripheral blood neutrophils were investigated. Functional activity of these cells was studied in 151 patients with odontogenic phlegmons and the data compared to those in 145 normal subjects. Assessment of leukocyte phagocytic activity by routine methods widely used in clinical practice did not show correlations of this parameter with the severity of the disease clinical course.

[The prevention and treatment of patients with suppurative-inflammatory diseases of the maxillofacial area by using vibromassage at autoresonance frequencies].

Autoresonance frequency vibromassage sessions were administered to patients with pyoinflammatory processes in maxillofacial soft tissues. Comprehensive examinations of these patients were carried out to assess the efficacy of this treatment modality; the examinations included rheographic and ultrasonic examinations of blood flow intensity, analysis of neutrophilic activity by chemiluminescence amplitude, measurements of trypsin inhibitor levels and cholinesterase activity, assessment of wound exudate pH values and of resorption capacity of purulent wound tissues. Microbiologic, histologic, cytologic, clinical and biochemical studies were carried out.

[A method for assessing the patient's clinical status and for predicting the clinical course of suppurative-inflammatory processes using chemiluminescence].

A new method for the assessment of the severity of the patients' status and prediction of the clinical course of suppurative-inflammatory processes is described, making use of the peripheral blood luminol-dependent chemiluminescence. Early assessment of the patient's condition and prediction of the disease course helps correctly choose the treatment strategy. The technique is simple and rapid, recommending its wide clinical application.

[The physiological adaptation functions of cellular and humoral immunity in patients with phlegmons of the maxillofacial area].

In order to evaluate the necessity in immunocorrective therapy the patients with maxillofacial phlegmons were tested for their cellular and humoral immunity. A slight increase or decrease in immunity indices before surgery in several patients were not beyond the physiological norm. Postoperative repetitive investigations the immune parameters attained the mean levels or even went higher without immune-correcting therapy.

[A method for creating an experimental model of abscess].

A technique of 7-day formation of an intramuscular abscess which is clinically and morphologically similar to that frequently encountered in clinical practice has been tried on 39 rabbits. The standard model proposed involves the following stages: trauma of the spinal erector 2.5-3 cm to the right or to the left from the midline between the second and fourth lumbar vertebra which is produced by a lumbar puncture under either anesthesia with a pulp extractor brought into the muscle up to 1.

[Ultrastructural manifestations of the membrane activity of neutrophilic leukocytes].

Phagocytic function of neutrophils is associated with activation of cell membranes. Neutrophil membranous activity is considered for patients with inflammatory and purulent diseases. It appeared not uniform and served the basis for division the neutrophils into three groups.

[Functional activity of neutrophilic leukocytes of the blood in patients with phlegmons of the maxillofacial area].

Phagocytic capacities of segmented and rod neutrophils were examined in in vitro studies of whole blood samples taken at weekly intervals from 32 patients with mild to severe phlegmons of odontogenic origin; 25 blood donors served as controls. The ability of neutrophils to take up and digest bacteria was found to be less marked the more severe the disease although the number of actively phagocytizing cells was increased. In some cases of purulent inflammation, though, the neutrophils showed a higher bactericidal potential as compared to the controls.

[A method for determining the phagocytic activity of polymorphonuclear leukocytes].

The proposed method is said to provide more precise and reliable information about phagocytic properties of PNL at the light-optical and electron-microscopic levels than do the other currently available and widely used techniques.

[Fluorometric method of determining prolylendopeptidase activity in human erythrocytes in normal and pathologic conditions].

A highly sensitive fluorometric method for determination of prolylendopeptidase (PE) activity in human erythrocyte hemolysates in the presence of hemoglobin has been developed. The method is based on measurement of fluorescence of 4-methyl-7-aminocoumarine released in the course of enzymatic reaction from the substrate Z-glycyl-proline-4-methylcoumarine-7-amide. A correlation was introduced for the quenching of fluorescence by hemoglobin.