[Monoclonal antibodies to rubella virus glycoprotein E1].

Aim: To obtain monoclonal antibodies (MCAs) to glycoprotein E1 of rubella virus, to assess their immunochemical characteristics and ability to use fluorescent MCA for rapid identification of rubella virus.

Materials And Methods: Rubella virus strain C-74 (Moscow), vaccine strains "Orlov" (Saint-Petersburg), Wistar RA 27/3 (USA) as well as strain Judith (Germany) were used. Viral antigens were obtained using diploid cells L-68 and cell lines VNK-21-F and Vero E6.

[Diagnostic value of enzyme immunoassay test for the detection of specific IgM antibodies to measles virus].

The diagnostic value of enzyme immunoassay systems manufactured by different companies was comparatively studied to detect specific IgM antibodies to measles virus. The assay systems were shown to have a high sensitivity. The specificity was higher in the assay systems designed on the capture principle, a type of solid-phase enzyme immunoassay.

[Characterization of antirubella vaccine in an epidemiological trial].

The work was aimed at the evaluation of the antigenic activity and reactogenicity of antirubella vaccine, manufactured by the Serum Institute of India, on the basis of the active observation of 373 children. Vaccinal reactions were registered in 8.8% of the vaccinees.

[Comparative evaluation of neurovirulence of domestic and foreign live mumps vaccine].

Morphological and immunofluorescent study of changes in the central nervous system of monkeys with mumps was carried out in order to determine the criteria of neurovirulence of different mumps virus strains. Quantitative evaluation showed a lower residual neurovirulence of L-3 strain vs. Jeryl Lynn and Urabe Am9 strains.

[Rubella in Russia: the detection of congenital rubella and the vaccination strategy and procedures].

The work on the evaluation of the role of rubella infection in the development of congenital malformations in newborn infants, as well as the immunogenic activity and tolerability of live attenuated vaccine Rudivax (Pasteur Mérieux Connaught) was carried out in the process of selective immunization in the Perm region. The study made it possible to find out 15% of malformations in the structure of the congenital pathology of newborn infants, which appeared due to the infection of the fetus with rubella virus and were manifested by multiple development defects, congenital C.N.

[Study and assessment of the antigenic activity and reactivity of rubella vaccine].

A total of 3422 adolescent girls and women of a reproductive age were vaccinated against rubella with French vaccine. Antigenic activity and reactogenicity of the vaccine were assessed in 97 girls, 100% seroconversion and weak reactogenicity was observed in seronegative vaccinees.

[Comparative testing of live measles vaccines manufactured in France, Japan, and Russia].

Live measles vaccines prepared from different strains in France, Japan, and Russia were compared at the L. A. Tarasevich State Institute for Standardization and Control of Viral Preparations.

[Evaluation of the reactivity and antigenic activity of the live measles vaccine, Leningrad-16, in different regions].

The reactogenicity and antigenic activity of live measles vaccine prepared from strain Leningrad-16 were assessed for 4 territories, two of which were exposed to radiation contamination as a result of the Chernobyl accident. The study was carried out at the L. A.

[A morphological and immunofluorescent study of the measles vaccinal process in an experiment on monkeys].

A correlation between localization of morphological lesions and the presence of viral antigen in the CNS was established. Dissemination of the infectious process in the CNS depended on both topography of the inoculated areas and more so on traumatic injury of the ventricular system. Viral antigen could be detected in the reticuloendothelial system and epithelial cells of the respiratory tract without inducing specific cytopathic changes.

[Characteristics of the immune response of children in the Arctic to live measles vaccine].

The intensity of immune response to live measles vaccine varies in children living in different climatic and geographical regions. The least intensive immunogenesis is registered in children living in the Arctic regions. The level of seroconversion in children living in these regions rises in response to measles vaccine containing a 10-fold amount of the virus per immunization dose.

[Study of the correlation between neutralization tests and hemagglutination inhibition tests in determinations of mumps virus antibodies].

In order to find out possibilities of wide application of the hemagglutination-inhibition (HI) test for determination of antibody to mumps virus, correlations between neutralization test (NT) and HI test was studied. Antibody to mumps virus was detected by parallel titrations of sera and gamma globulins in HI and NT tests in two experimental series each of which used various modifications of these tests. Statistically significant strong correlation was established between these tests.