[T-cell responsiveness to specific group A streptococcus antigens in patients with recurrent erysipelas].

Peripheral blood leukocytic migratory activity (LMA) was studied in 51 patients with recurrent erysipelas versus 63 patients with primary erysipelas. To reveal LMA, the authors employed in vitro a screening cell migration test as an indicator of the cooperation of T and B lymphocytes and macrophages, by stimulating with polysaccharide A, surface proteins, L-antigen, hyaluronidase, streptolysine-O, and a complete set of Grasse S. pyogenes.

[Prognostic value of leukocytic migratory activity indices in patients with erysipelas].

Peripheral blood leukocytic migratory activity (LMA) was studied in patients with primary or recurrent erysipelas. A screening cell migration test (SCMT) was used in vitro and it established the prognostic value of MAL parameters at week 1 after the onset of erysipelas. It has been shown that a rapid transition of LMA from the phase of acceleration to that of inhibition characterizes the formation of an adequate immune response, corresponds to the good course of the disease, and has a low likelihood of recurrences.

[T-cell responsiveness to specific group A streptococcus antigens in patients with primary erysipelas].

Peripheral blood leukocytic migratory activity (LMA) was studied in 63 patients with primary erysipelas. To reveal LMA, a screening cell migration test (SCMT) was used as an indicator of the cooperation of T- and B-lymphocytes and macrophages in the stimulation with polysaccharide A, surface proteins, L-antigen, hyaluronidase, streptolysin O, a complete S. pyogenes antigen complex after Grasse.

[Cell-cooperation integrated test for the detection of immune reactivity to Shiga-toxin in patients with acute intestine infection].

Described are the results of approbation of the cell-migration screening test reflecting the outcome of cooperation between T- and B-cells and macrophages. It was shown, in adults and children with intestine infection, to be a highly-effective tool for the detection of suppressed immune response during early disease stages at stimulation in vitro by Shiga toxin at nano- and picogram concentration; it can also be used for the evaluation of the shaping specific anti-Shiga-toxic immune response. The parameters of the migration activity of peripheral-blood leucocytes at exacerbation and convalescence were demonstrated to correlate with the age of sick children and with the severity of intestine infection as well as with the level of a Shiga-like toxin detected in coprofilters and circulating immune complexes.

[Dermatoglyphic characteristics in hereditary ichthyosis].

Examination of 530 dermatoglyphic patterns of the palms and fingers in 265 patients with 5 nosologic forms of hereditary ichthyoses (autosomal dominant ichthyosis vulgaris, X-linked, congenital, lamellar, epidermolytic ichthyoses) have revealed significant differences in the pattern intensities and in the incidence rate of certain types of these patterns, associated with this or that form of ichthyosis; abnormalities in the flexor wrinkles of the ridge skin have been observed in all the studied forms of the disease, except the X-linked condition. The studies have revealed an abnormal roughness of the papillae on the epidermal ridges in epidermolytic ichthyosis and an obliterated dermatoglyphic pattern in lamellar ichthyosis. The detected changes in the ridge skin and the dermatoglyphic phenotypes may be useful for the differential diagnosis of these conditions.

[Characteristics of keratoconjunctivitis sicca in Sjögren's disease and syndrome].

The paper is devoted to an analysis of the clinical manifestations of keratoconjunctivitis sicca in Sjogren's syndrome in combination with SLE, sclerodermia systematica, rheumatoid arthritis, and in Sjogren's disease. Some characteristic signs of a course of keratoconjunctivitis sicca in Sjogren's disease and syndrome were defined.

[Principle of the supramolecular stacking of the cellular deoxyribonucleoprotein complex].

Using cytofluorimetry with acridine orange staining and a modified thermal denaturation technique of cellular DNP, it has been shown that chromatin melting profiles of normal human nuclei (from lymphocytes and granulocytes) have distinct regularities. It is believed that these regularities reflect specific supramolecular chromatin organization. Parallel comparative analysis performed using electrophoretic fractionation and isoelectric focussing of nuclear proteins has revealed that: 1) peculiarities of chromatin melting profiles are independent of the quantity and molecular weights of chromatin proteins; 2) the lack of principal differences in chromatin melting profiles and the data on isoelectric points of nuclear proteins of granulocytes and lymphocytes from the same patient indicate that specific supramolecular organization of DNP-complex depends on the chromatin protein charge.

[Structure of the interphase chromatin in the peripheral blood cells of children with acute lympholeukemia and in their healthy parents].

It has been shown using labelled modified AO cytofluorometry of DNP cellular thermal denaturation that the melting profiles of peripheral blood cellular chromatin in children with acute lympholeukemia (ALL) were of strictly individual, "unclassifiable" nature and were similar to those of their mothers but different from those of their fathers and healthy people, which seems in favour of a possible connection between the disease under study and the peculiarities of the mother's genotype. Similar types of deviations have been found in the structure of interphase chromatin of healthy parents of children with ALL. Such a combination of changes in the parents' genotype may prove unfavourable, increasing the birth rate of neonates with a genetic predisposition to the disease in question.

[Interphase chromatin of the peripheral blood cells of patients with a chronic form of myeloid leukemia (Ph+)].

It was shown with labeled AO cytofluorometry using the authors' modification of DNP cell thermal denaturation that in untreated patients with chronic myeloid leukemia (CML) in the blast crisis phase, the melting profiles of peripheral blood cell chromatin were "unclassified", strictly individual in nature (unlike the identical parameter in healthy subjects) that may attest to regulatory disturbances of the genetic apparatus. The demonstration of the "normalization" of the melting profiles of chromatin in CML patients during the clinico-hematological compensation of the disease makes it possible to suggest that luminescent fluorometry (in the authors' modification of DNP cell thermal denaturation) should be used for the treatment of hemoblastoses in order to study the genesis and time-course of the diseases running their course with remissions and as criterion of drug therapy of these diseases.

[Interphase chromatin in lymphocytes in sex differentiation disorders].

Interphase chromatin of peripheral lymphocytes was studied in patients aged 6 to 20 years with Turner and Morris's syndromes by AO labeled fluorometry using the authors' modification of DNP cell thermal denaturation. It was shown that the lymphocyte chromatin melting profiles represent the curves with seven maxima at the following temperatures: 47 degrees, 55 degrees, 65 (+/- 2)degrees, 78 (+/- 1)degrees, 82, 88 (+/- 1)degrees, 92 (+/- 2)degrees C (P less than 0.01).

[Interphase chromatin of human cells with diploid and haploid genomes].

A study was made of the chromatin structure of mature sperm cells from healthy males aged 25 to 40 using fluorescent microscopy and acridine orange staining according to the DNP cell thermal denaturation technique modified by the authors. It was shown that normal human sperm cell chromatin melting profiles represent uniform curves with maxima in the following temperature ranges: 43 (+/- 2) degrees, 55 (+/- 1) degrees, 67 (+/- 2) degrees, 77 (+/- 1) degrees, 82 (+/- 0.5) degrees, 89 (+/- 1) degrees, 92 (+/- 2) degrees (P less than 0.

[Structure of interphase chromatin of diploid and trisomal human cells studied by the technic of thermal denaturation in media of low ionic strength].

Fluorescent microscopy with the use of acridine orange has shown on a short-term human cell culture that the melting profiles of intact lymphocytes from normal subjects present the curves with maxima (F530) within a definite temperature range: 45, 65, 78, 85, 88 and 92 degrees C (+/- degrees C). The melting profiles of lymphocytes from patients with the Down syndrome present the curves with maxima at 65, 85, 88 and 92 degrees C. The melting of human cells in low ionic strength media as compared with physiological one causes the disappearance of the maxima on the chromatin melting curve and disappearance of the difference in the melting profiles of cell chromatin from normal subjects and patients.

[Computer classification of the fusion profiles of human lymphocyte interphase chromatin].

AO fluorescent microscopy coupled with thermal denaturation of DNP cells as modified by the authors was used to study the structure of lymphocyte interphase chromatin from 164 normal persons. Analysis of the data processed by means of a Sperry Univac Computer 90/30 has demonstrated that in 40% of the cases, the melting profiles of DNP cells from normal persons represent, irrespective of the sex, a complicated but consistently repeated curve with 6 peaks at certain temperatures, i.e.

[Melting profiles for lymphocyte chromatin of healthy subjects and Down's disease patients obtained by the technic of cellular DNA absorption in ultraviolet light].

Integral values of optical density (E260) were obtained for lymphocyte nuclei from normal people and those suffering from Down's syndrome during the melting of cells in media of varying ionic strength (0.15 M NaCl - control; 0.015, 0.