[Comparison of different G-CSF treatment effectiveness in experiments on irradiated mice].

In the experiments on F1 (CBA x C57BL) and BALB mice irradiated by 137Cs gamma-rays, preparations of unglycosilated G-SCF such as Neupogen and their domestic analogs Leucostim and Neupomax were investigated. The tests such as 9-day bone marrow cellularity (BMC) and endogenous CFUs, the neutrophile number restoration, the 30-day survival index have shown that all three preparations have an approximately equal effectiveness relating to acute radiation disease treatment and granulopoiesis stimulation after a 5-10 day consecutive administration following irradiation of mice at lethal and sublethal doses. We have come to the conclusion that Leucostim and Neupomax can be regarded as adequate substitutes for Neupogen.

[Mesenchymal stem cells transplantation in the treatment of radiation skin lesions].

Transplantation of mesenchymal stem cells, both at early and later stage after local exposure of rats source beta radiation dose, 90Sr/90Y 140 GR, stimulates recovery of damaged skin. Diminution area local radiation injuries and accelerate healing radiation ulcers. Clinically shows the high efficiency of the transplantations autologous mesenchymal stem cells in treatment of deep beam ulcers, intractable standard conservative treatment.

[Antiradiation effects of vaccine Grippol].

The propose of these examination was the study of antiradiation effects of vaccine Grippol (VG). It was determined, that subcutaneous injection VG mice (0.2 ml), hamsters (0.

[The influence of multipotent mesenchymal stromal cells of bone marrow on process of local radiation injury in rats after local beta-irradiation].

The influence of multipotent mesenchymal stromal cells (MMSC), got from allogenic bone marrow, on local radiation injury in rats after beta-irradiation by a source 90Sr/90Y in a dose 140 Gy under various conditions and ways transplantation was studied. It was established, that transplantation MMSC, allocated on biodegradative membranes, which have been carried out for 21 day after an irradiation in conditions minor surgical of ulcers, resulted in reduction of the area of local beam defeats and acceleration of healing of skin, in comparison with the control of an irradiation. The introduction of suspension MMSC subcutaneous around of the center of a defeat at 8 day after an irradiation caused earlier healing of ulcers.

[Antiradiation effects of lactoferrin].

The propose of these examination was studied antiradiation effect of lactoferrin (LF). It was determined, that subcutaneous injection LF (65 or 300 mkg/kg) guinea-pigs irradiated after 1-14 days after of total gamma-irradiation (2.5 Gr) had therapeutically effect attached to the bone marrow form of acute radiation disease (ARD).

[The influence of mexidol on post-radiation recovery of hemopoietic system].

The purpose of this work was the study in experiments on mice of influence of mexidol on current and outcome of an acute radiation sickness. At an irradiation in a dose 6.0 Gy was studied a condition of hemopoietic system, and at an irradiation in a dose 7.

[Recombinant human interleukine-1beta (betaleukine) usage for acute radiation sickness of severe degree treatment at canines].

Recombinant human interleukine-1beta (betaleukune) of Institute of especially pure biopreparation production had been examined as a treatment means at acute radiation disease of severe degree at dogs. Dogs were irradiated totally in doses above the LD95/45. Betaleukine had been administered s/c twice in day in 15 min - 2 h after irradiation.

[Acute radiation sickness in experiment. Efficacy of mexidol].

Experiments on mice were made to examine mexidol effects on the course and outcome of acute radiation sickness (ARS). Regenerative processes in hemopoietic system were studied after 6.0 Gy radiation, survival of the animals was studied in 7.

[Effect of mexidol on hemopoietic system in conditions of an emotional stress after exposure to ionizing radiation].

The emotional stress after an irradiation can complicate the current of radiative defeats. At an emotional stress developing in early terms after an irradiation in low doses, are reduced adaptive and compensator capabilities of hemopoietic system. The emotional stress after a lethal dose irradiation inhibits the post-radiation recovery of haemopoiesis, aggravates the course of acute radiation disease and decreases the efficiency of the radioprotector--indralin.

[The effect of prodigiozan on the postradiational recovery of hemopoiesis in long-term bone morrow cultures of mice of different genotypes].

The influence of prodigiozan on the processes of postirradiation recovery of hemopoiesis in long-term bone morrow cultures of two strain mice, having genetic distinctions in the condition of systems of the reparation DNA was investigated. It was showh, that the irradiation of long-term bone morrow cultures of mice reparation-defective strain 101/H resulted in the greater degree damage of early haemopoietic precursors (GM-CFC), reduction of the amount of the immature and of the mature granulocytes and of the decrease of the number of stromall cells in the comparison with the bone morrow of reparation-capable mice (CBA x C57B1)F1. Under the introduction in cultures of prodigiozan for 24 hours prior to an radiation the distinctions of the speed of postirradiation recovery of hemopoiesis substantially smoothed out, and the protective effect of the drag in bone morrow cultures of mice 101/H was comparable to those, marked in bone morrow cultures of reparation-capable strain mice (CBA x C57B1)F1.

[The adaptive reactions in hemopoietic system of the irradiated mice of two lines, differing in the level of emotionally-stress reactions on the emotional stress].

On lines of the mice genetically differing on the sensitivity on emotional stress (steady--of the mouse of the line C57BL/6 or sensitive--of the mouse of the line BALB/c), the research of the development of the adaptive reactions in the system of the blood on the emotional stress influencing animals on the background of the preliminary irradiation in a low dose (0.9 Gy) is carried out. The unirradiated mice of the line C57BL/6 adequately reacted on the stress, that was shown in a moderate behavioural reaction during the period of stress, and the development of high-grade adaptive reactions in the hemopoietic system.

[Using aphobazol for treatment of the emotional stress after exposure to ionizing radiation].

Emotional stress after radiation may aggravate the course of radiation-induced diseases. The use of the selective anxiolytic aphobazol (a drug from a mercaptobenzamidasol group) corrects development of adaptive reactions to stress in the blood system and normalizes its compensatory potentialities in animals under conditions of combined influence of intensive long-term emotional stress and low-dose irradiation. In lethally irradiated animals the treatment of stressed animals with aphobazol favourably influenced the course of acute radiation disease, enhanced recovery processes in the blood system, increased the animals' survival.

[Antiradiation effects of leucynferon on dogs and guinea pigs].

In experiments with two species of animals (dogs, guinea pigs) irradiated with sublethal and lethal doses of gamma-rays, it was observed, that leucynferon had antiradiation effect. Course of injections: dogs--8 injections subcutaneus: 2.0 ml (1, 3, 5, 8, 12, 14, 21, 34 days after irradiation); guinea pigs--14 injections subcutaneus, 0.

[The role of hematopoietic microenvironment in prodigiozan' mechanism of action on postradiation recovery of hemopoiesis in long-term bone marrow cultures].

Influence of prodigiozan in different concentrations on the process of postirradiation recovery of haemopoietic precursors (GM-CFC) and morphologically recognizable elements of the bone marrow were studied in long-term bone marrow cultures during 28 days after 2 Gy gamma-irradiation. Also, prodigiozan was studied for action on the contents of GM-CFC and induction of GM-CSF three, 24 and 48 hours after injection in cultures unexposed to radiation. It is shown that injection of prodigiozan in concentration 0.

[Role of hematopoietic microenvironment in the mechanism of radioprotective action of interleukin-1 beta in long-term bone marrow cultures].

The influence of human interleukin-1 beta in different concentration on processes of postirradiation recovery of haemopoietic precursors (GM-CFC) and morphology of recognized elements of bone marrow were studied in long-term bone marrow cultures during 28 days after gamma-irradiation with a dose of 2 Gy. It was studied also the action of interleukin-1 beta on proliferation, the contents of GM-CFC and the induction of GM-CSF in non-irradiated cultures. It was shown that the injection of interleukin-1 beta increased proliferation and the content of GM-CFC and also raised an induction of GM-CSF in the non-irradiation cultures.

[Bone marrow hemopoiesis at emotional stress reactions of various intensity upon a background of exposure to low dose ionizing radiation].

Rats preliminary exposed to 0.9 Gy of ionizing radiation showed disturbances in the development of adaptive reactions of blood system to emotional stress, compensatory capacity of the blood system being decreased. A degree of the disturbances directly depended on the duration and intensity of the emotional stress: at the prolonged intensive emotional stress a sharp decrease in the adaptive and compensatory capacity of blood system was found; at short intensive or moderate stress the changes in the adaptive and compensatory capacity of hemopoiesis were less pronounced.

[Pharmacological correction of emotional stress after exposure to ionizing radiation and treatment with a radioprotector indralin].

A possibility to use tranquilizers (aphobazole, phenazepamum) for reducing symptoms of prolonged emotional stress influencing rats after irradiation under treatment with a radioprotector indralin was investigated. It was found that indralin showed the protective effect and activated regenerative processes in the hemopoietic system of animals exposed to doses of 6.0 and 7.

[Effect of beta-interleukin on the content and proliferation of hemopoietic precursor cells in intact and irradiated long-term cultures of the bone marrow].

Effects of beta-leukine (human recombinant interleukin-1 beta) on the number and proliferative activity of early hemopoietic precursor cells (CFU-GM) and colonystimulating activity (GM-CSF) were studied in long-term cultures of the bone marrow. Studied were also recovery of CFU-GM, total number of myelocaryocytes and content of immature and mature granulocytes in response to beta-leukine after gamma-radiation of cultures in a dose 2.0 Gy.

[Disruptions in the blood system upon exposure to low-dose ionizing radiation depending on duration of emotional stress].

In experiments on rats, disturbances in the development of blood system adaptive reactions on emotional stress were found. Under the combined effect of preliminary (before stress) exposure to 0.8 Gy of gamma-radiation and the emotional stress of various duration (2, 4 and 8 days), a compensatory capability of the blood system decreased.

[Dependence of the therapeutic effectiveness of interleukin-1beta on the time of the drug administration after irradiation of mice].

In experiments on mice F1(CBA x C57BL/6) the dependence of 30th days survival on the time of betaleukin (medicine form of interleukin-1 beta) administering after exposure to 7.5 Gy whole body gamma-irradiation from 137Cs (approximately DL80/30) was studied. Betaleukin was injected subcutaneously in dose 25 mcg/kg 0.

[Study of mechanisms of anti-irradiation effects of interleukin-1beta in long-term bone marrow cultures].

The influence of betaleukin (human recombinant interleukin-1 beta) on the processes of postirradiation recovery of haemopoietic precursors (GM-CFC) and the level of granulocyte-macrophag colony-stimulating factor (GM-CSF) were studied in long-term bone marrow cultures after gamma-irradiation with a dose 2 Gy. Then the betaleukin action on the contents of GM-CFC and induction of GM-CSF in the non irradiated cultures was studied. It was shown that betaleukin increased the induction of GM-CSF and raised the contents of GM-CFC in long-term bone marrow cultures, and the maximal increase of a GM-CSF level and GM-CFC amount was marked in 20 hours after introduction.

[Emotional stress and problems of radiation medicine].

The influence of emotional stress on behaviour and industrial activity of man as well as on the development of posttraumatic stressful frustration and other disorders is considered in connection with real or possible action of ionizing radiation, and on the course of radiation injuries. It is shown that a problem of emotional stress and radiation safety are closely connected. The study is important for solving problems facing emergency medicine.

[Bone marrow hematopoiesis in polymer composite implants in long-term bone marrow cultures].

The morphological composition of cells on implants made of different composition materials was studied on the model of long-term bone marrow cultures at cultivation for 28 days. Three samples of materials were investigated: polymethyl methacrylate (PMMC), PMMC with hydroxyapatite (HA) and PMMC with HA and subsequent processing with supercritical carbon dioxide. A gradual rise in total number of cells was observed on the implants for 4 weeks as well as increasing number of stromal and hemopoietic cells.

[State of hemopoiesis under exposure to low doses of ionizing radiation and emotional stress during treatment with anxiolytic aphobazole].

In experiments on rats it was found that aphobazole administered before emotional stress is capable to stop violations of adaptive reactions and compensator capabilities of a hemopoietic system in conditions of development of an emotional stress in early terms after gamma-irradiation with a dose of 0.9 Gy.

[The cellular and humoral mechanisms of the radioprotective action of prodigiozan and indometofen on long-term mouse bone marrow cultures].

Effects of prodigiosan and indometophen on the contents and proliferative activity of CFU and CFU-GM as well as GM-CSF prior to radiation were studied in long-term cultures of murine bone marrow. 24 hours and 5 days after administration of prodigiosan and indometophen, respectively, the long-term cultures were exposed to ionising radiation in a dose 2.0 Gy.