[Structure of lymphoma gangliosides in EL-4 mice].

The structure of the major gangliosides from murine lymphoma EL-4 was established by acid hydrolysis, methanolysis, methylation analysis, neuraminidase treatment and chromium trioxide oxidation. Two of these gangliosides were identified as the N-acetyl and N-glycoloyl forms of the GM2 ganglioside. The third ganglioside making up to 40% of the total ganglioside pool contained two sialic acid residues and was identified as a GD2 ganglioside which up to now had not been reported in extraneural tissues.

[Gangliosides of pig and human thymuses].

The ganglioside composition of thymuses from pigs of different age, of embryonal human thymus and of thymus from newborns was studied. Pig thymus was found to contain 8 ganglioside fractions, i. e.

[Gangliosides of mouse liver and ascites hepatoma 22a].

The ganglioside composition of the ascite hepatoma 22a and that of mouse liver was compared. GM2 was shown to be the main ganglioside in both tissues; however, the gangliosides of the hepatoma differed from liver gangliosides by a higher amount of disialoganglioside GD1a and by contents of monosialoganglioside GM1 and disialoganglioside GD1b which are absent in normal mouse liver. Trisialogangliosides which are characteristic for normal liver cells were not detected in the hepatoma.

[Neutral glycolipids of the calf thymus and normal and leukemic bovine lymphocytes].

The composition and structure of the neutral glycolipids of calf thymus and of lymphocytes isolated from the blood, lymph, lymph nodes and spleen of normal and leukemic cows were investigated. The main neutral glycolipids of all lymphocytes studied were identified as glucosyl, lactosyl and digalactosylglucosylceramides. The relative amounts of these glycolipids were found to depend on lymphocyte localization.

[Structure of gangliosides of normal and leukemic bovine blood lymphocytes].

The structure of main gangliosides from normal and leukemic bovine blood lymphocytes was established. N-Glycoloylhematoside and a ganglioside containing N-glycoloylneuraminic acid and an oligosaccharide chain identical to that of erythrocyte sialosylparagloboside were found to be present both in normal and in leukemic bovine blood lymphocytes. In addition, the leukemic lymphocytes contained a ganglioside identical to N-acetylneuraminosylparagloboside and at least two disialosyllactosylceramides containing N-acetyl- and N-glycoloylneuraminic acids.

[Ganglioside composition of normal and leukemic bovine lymphocytes].

The ganglioside composition of bovine peripheral lymphocytes was shown to change sharply under lymphoid leukemia. In normal lymph, lymph nodes, spleen and blood lymphocytes the major ganglioside is N-glycolylhematoside, whereas in calf thymus lymphocytes appreciable amounts of more polar components (GM1- and GD1a-like gangliosides) were found. In leukemic lymphocytes isolated from the same tissues the hematoside content is decreased, while the amount of more polar gangliosides is increased.

[Phospholipid composition of lymphocytes from normal and leukemic blood, lymph, lymph nodes and spleen of cattle].

The phospholipid composition and cholesterol content of lymphocytes from bovine blood, lymph, lymph-nodes and spleen were studied. The cholesterol/phospholipid molar ratio in the normal lymphocytes of blood and spleen was found to be about two times higher than that in the normal lymphocytes from lymph and lymph-nodes. On lymphocytic leukemia the cholesterol/phospholipid molar ratio of the blood lymphocytes decreased sharply, whereas that in lymph and lymph-nodes lymphocytes was unaffected.