[Plasmapheresis in combined treatment of acute rejection crises after kidney transplantation].

The aim of the study was assessment of effectiveness and safety of extracorporeal procedures (plasmapheresis) in prevention and combined treatment of acute rejection crises (ARC) after kidney transplantation in patients at high risk to develop acute immunological conflicts in early postoperative period. 94 patients aged 21-56 years after allotransplantation of cadaver kidney (ATCK) entered the study. They were divided into two groups: group 1 patients (n = 47) received plasmapheresis procedures in combined preventive and therapeutic treatment of ARC; matched group 2 patients (n = 47, retrospective) received the same therapy but plasmapheresis.

[Recombinant Thermus thermophilus His6-DNA polymerase with reverse transcriptase activity].

The Tth DNA polymerase genes from the thermophilic bacterium Thermus thermophilus (strans HB-8 and Tt-111) were cloned and expressed in Escherichia coli cells. The target protein contained a polyhistidine tag at the N-terminus that allowed its single-stage isolation by affinity chromatography on Ni-NTA-agarose. The isolated enzyme displayed both high DNA polymerase and reverse transcriptase activities.

[Membrane permeability in combined arterial hypertension and pathology of the digestive organs (based on epidemiological study data)].

An epidemiological study of arterial hypertension and digestive diseases revealed 27% patients with concurrent abnormalities. It was shown that determination of membranous lesions by the rate of red blood cell Na-Li contratransport may be useful in the diagnosis of syntropy.

[Cloning the asd and lysC genes from Cornyebacterium glutamicum].

Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.

[Study of the regulation of crp gene expression in Escherichia coli K12].

The regulation of crp gene expression by CRP-cAMP complex was studied in E. coli strain by the crp-lac operon fusion. F'141 crp+ episome decreased 5-7 fold the high level of crp-lac expression in crp strains while F'141 crp episome had no effect.

[The epidemiological aspects of combined arterial hypertension and peptic ulcer].

Altogether 598 workers and employees of an industrial enterprise underwent epidemiological examination. The age of the examined ranged within 30 to 49 years. Arterial hypertension was revealed in 14.

[Study of the expression of the cya gene in Escherichia coli K12 on a model of the hybrid cya-lac operon].

Bacteriophage Mud1 (Ap, lac) was used to construct the cya-lac operon fusion in Escherichia coli chromosome. The maximal cya expression was observed by measuring the levels of beta-galactosidase in the strains lacking the cAMP. The inhibition of cya transcription was proportional either to the amount of cAMP added or to the copy number of cya gene in the cell.

[Isolation of mutants with impaired retroinhibition of histidine biosynthesis].

The Escherichia coli, strain possessing purF, deoD and add mutations converts exogenous adenine into guanine nucleotides exclusively by the pathway coupled with histidine biosynthesis. When grown on adenine, this strain demonstrated sensitivity to histidine, thus making it possible to select histidine-resistant hisGR mutants with ATP-phosphoribosyltransferase desensibilized for histidine. The hisGR mutations were obtained in two his operons introduced into the his operon-sensitive E.

[Cloning and expression of the histidine operon of Salmonella typhimurium in cells of Escherichia coli].

The histidine operon of Salmonella typhimurium and its fragments were cloned in Escherichia coli cells on a multicopy plasmid. Expression of the cloned genes and histidine production by the variants possessing the hisG mutation which desensibilizes the ATP phosphoribosyl transferase for histidine were studied. Amplification of the complete operon including the hisG gene enables histidine accumulation of 2-3 g/l after 72 hours of fermentation.

[Metabolic regulation of the histidine operon in Escherichia coli and Salmonella typhimurium].

Expression of the histidine operon in Escherichia coli cells in contrast to the one in Salmonella typhimurium is changed proportionally to cells growth rate on the different carbon sources. The specific activity of histidinol-dehydrogenase is repressed by addition of 19 amino acids both in Escherichia coli and Salmonella typhimurium independent of the growth medium used. Using of Escherichia coli and Salmonella typhimurium strains containing the heterologous histidine operons made possible to demonstrate the dependence of the histidine operon metabolic regulation to be determined by the operon itself but not by the specificity of the recipient cells.

[Prospective epidemiologic study of arterial hypertension in an organized male population].

The effect of active arterial-hypertension preventive programs on the epidemiologic situation was studied over a nine-year period, 1977 through 1985. In the first five years, the number of effectively treated cases increased, while essential hypertension morbidity associated with temporary disability diminished, as did mortality and the incidence of myocardial infarction and cerebral accidents among 40- to 54-year-old male industrial workers. After an active preventive program was abandoned at one factory, the parameters examined deteriorated considerably, and a sample selected for comparison showed an abrupt drop in treatment efficiency within three years after annual screenings were discontinued.

[Primary structure of the alpha-subunit of Na+,K+-ATPase from the swine kidney. III. Complete nucleotide sequence corresponding to the structural region of the gene].

The nucleotide sequence of the cDNA, containing coding region of the alpha-subunit of the pig kidney Na+, K+-ATPase, was determined. The region contains 3063 b.p.

[Genes encoding the beta-subunit of bacterial RNA-polymerases. I. Primary structure of the EcoRI-C fragment of the Salmonella typhimurium gene rpoB].

BglII fragment of S. typhimurium DNA, containing rpoB gene coding for the RNA polymerase beta-subunit, was cloned. The nucleotide sequence of the rpoB gene EcoRI-C fragment (2873 b.

[Construction of the hybrid crp-lac operon and study of the role of CRP-cAMP complex in its regulation in Escherichia coli].

Phage Mu d1(Ap,lac) was used to construct the hybrid crp-lac operon in Escherichia coli cells possessing crps mutation which increases sensitivity of bacterial growth to exogenous cAMP. Transcription of the structural lac genes in this hybrid operon was initiated from the promoter region of the crp gene. By measuring levels of beta-galactosidase enzyme, the maximal transcription of the crp gene was obtained in strains with cya or crp mutations causing inactivation of the CRP - cAMP complex.

[Prospective epidemiological study and the effectiveness of the prevention of arterial hypertension in an organized female population].

Altogether 3875 women aged 30 to 50 engaged in the textile industry, were examined. Primary prevalence of arterial hypertension was 7.0-7.

[Protective effect of alpha-tocopherol on the hydroxylating system in liver endoplasmic reticulum membranes against the damaging activity of hyperbaric oxygenation].

Effect of hyperbaric oxygenation (oxygen pressure 3039 hPa, exposition 3 hrs) on activity of the hydroxylating system and lipid peroxidation was studied in membranes of rat liver endoplasmic reticulum. Within the first 3 hrs after hyperbaric oxygenation the rates of aniline n-hydroxylation, amidopyrine N-demethylation, NADPH oxidation and the cytochrome P-450 level were decreased, while the rates of ascorbate- and NADPH-dependent lipid peroxidation were increased. Oil emulsion of alpha-tocopherol, administered per os into rats at a dose of 50 mg/kg every 12 hrs within 2 days before hyperbaric oxygenation, prevented the posthyperoxic deterioration of xenobiotic metabolism and the intensification of lipid peroxidation in liver microsomes.

[Cloning of the gene controlling catabolite repression with the participation of cyclic adenosine monophosphate in Escherichia coli K-12].

The crp gene coding for cyclic adenosine monophosphate receptor protein has been cloned on the vehicle pBR325 using restriction endonuclease PstI and the recipient strain C600 crp. The pCAP2 hybrid plasmid obtained has a molecular weight 7.0 MD and in the pBR325 with the insertion into a PstI site.

[Effect of hyperbaric oxygenation on the microsomal enzymes of the liver monooxygenase system in the rat].

Hyperbaric oxygenation of rats made at a pressure of 303.9 kPa (3 at. abs.

[Cloning of the adenylate cyclase gene of Escherichia coli K-12].

The adenylate cyclase gene of Escherichia coli has been cloned on the plasmid vector pBR325. The hybrid plasmid pTH4 obtained has a molecular weight of 6,4 megadalton and represents pBR325 plasmid with the insertion of 2,8 megadalton in the Pst1 site. The cya mutant bacteria carrying pTH4 recover their ability to utilize mannitol, lactose and other carbohydrates as carbon sources, and lose this ability again in the case of rare spontaneous excision of the DNA insert from the Pst1 site.

[Genetic nature of an Escherichia coli mutant characterized by high basal levels of catabolite-sensitive enzymes in a study using glucose as the carbon source].

The genetical nature of Escherichia coli GluR mutant was studied, the mutant having been characterized by an increased basal level of enzymes of the deo-operon (thymidine phosphorylase, in particular,-up to 8-10 times, in comparison to the wild type) and of some other catabolite sensitive enzymes. The above mentioned phenotype turned out to be caused by the presence in the genome of the GluR mutant of the ptsG mutation demonstrating close linkage (98% in P1 transduction) with the crp locus. The latter was designated in this work as crp-active (crpa).

[Realization of a program to control arterial hypertension in the industrial enterprises of the city of Cheboksary].

Epidemiological examination of males from 40 to 54 years of age at nine industrial enterprises revealed arterial hypertension in 19.5% of cases. As a result of intensive treatment of patients in a prophylactic cardiological department the incidence of arterial hypertension reduced, the patients learned more about their disease, the efficacy of treatment improved, and there was a decrease in losses of working time due to temporary disability of workers.