[Comparative analysis of informative-diagnostic properties of mucosa immune-reactivity parameters].

Aim: Study the features of immune-reactivity expression in mucosa depending on their topicity and etiopathogenesis of the pathological process.

Materials And Methods: Data from 30 clinically healthy children and 77 children with acute and recurrent diseases of respiratory tract: 51--with acute and 15--with chronic bronchitis; as well as 132 women: 41--with active stage of acute urogenital chlamydia infection, 29--with recurrent chronic process, 30--with non-recurrent form and 32 clinically healthy women were analyzed. Saline and urogenital tract mucosa discharge was analyzed for IgG, sIgA and secretory component, IL-1beta, 4, 6, 8, 9, 10, 12, IFNgamma, TNFalpha and GM-CSF, TLR-2, TLR-3, TLR-4, TLR-8 gene expression levels as well as content of lysozyme, total protein and leucocytes.

[Changes in the cytokine profile in residents with chronic obstructive lung disease exposed to urban air pollutants].

The complex of urban air pollutants was established to affect the cytokine profile in Moscow residents with chronic obstructive pulmonary disease (COPD)), as it causes a significant reduction of L-6 and TNF-alpha in their blood samples. The identified changes indicate to inhibition of the functional activity of cells of the reticuloendothelial system, which can lead to the persistence of seasonal bacterial-viral infections and more rapid chronization of the basic disease.

[The phylogenetic position of Chlamydia strains isolated from monkeys and humans with Chlamydial pathology in the family Chiamydiaceae. Genotypic and phenotypic properties of this pathogen].

Based on the results of the comparative analysis concerning relatedness and evolutional difference of the 16S - 23S nucleotide sequences of the middle ribosomal cluster and 23S rRNA I domain, and based on identification of phylogenetic position for Chlamydophila pneumoniae and Chlamydia trichomatis strains released from monkeys, relatedness of the above stated isolates with similar strains released from humans and with strains having nucleotide sequences presented in the GenBank electronic database has been detected for the first time ever. Position of these isolates in the Chlamydiaceae family phylogenetic tree has been identified. The evolutional position of the investigated original Chlamydia and Chlamydophila strains close to analogous strains from the GenBank electronic database has been demonstrated.

[Medico-Prophylactic Faculty of I. M. Sechenov Moscow Medical Academy and its achievements].

Major achievements of Medico-Prophylactic Faculty, I.M. Sechenov Moscow Medical Academy, for the period of 2000-2010 are reported.

[Architectonics of gastrointestinal microbial community].

Architectonics of gastrointestinal microbial community in colonic mucosa and feces was studied in patients with different gastrointestinal pathologies and without them. The study model was comprised of Bifidobacteria, Lactobacteria, E.coli, Enterococci, Staphylococci, and Candida.

[Management as a key professional competence of preventive medicine specialists].

The paper deals with the strategy of training specialists in preventive medicine and improvement of their professional skills for the management of activities aimed at the maintenance of adequate population health status and prevention of adverse effects of environmental factors. Integration of managerial issues into curricula and teaching courses for preventive medicine specialists may be instrumental in upgrading their professional level.

[Colonization resistance and immunological reactivity of children's oropharyngeal mucosa in health and bronchopulmonary pathology].

The study group was comprised of 27 practically healthy children, 51 patients with acute bronchitis, 15 with chronic bronchitis and 11 with pneumonia. It was shown that changes of microbiocoenosis in back of the throat (BOT) were related to increased mucosal contamination with normal microflora and opportunistic microorganisms. The highest degree of contamination was observed in children with acute bronchitis.

[Validation of the modified algorithm for predicting host susceptibility to viruses taking into account susceptibility parameters of primary target cell cultures and natural immunity factors].

The paper presents results of testing a modified algorithm for predicting virus ID50 values in a host of interest by extrapolation from a model host taking into account immune neutralizing factors and thermal inactivation of the virus. The method was tested for A/Aichi/2/68 influenza virus in SPF Wistar rats, SPF CD-1 mice and conventional ICR mice. Each species was used as a host of interest while the other two served as model hosts.

[The role of lectins from probiotic microorganisms in sustaining the macroorganism].

The notion of lectins has been expanded with special reference to lectins of probiotic microorganisms (LPM). New data on LPM and their properties are considered in the context of a new concept of systemic organization of LPM being developed. Conditions for complete separation of poly (N-acetylgalactosamine-?)-binding lectins and polyMan-?/oligomannoside/mannan-binding lectins are developed on the assumption that they contain a single carbohydrate-binding site in their structure.

[Clinico-laboratory associations of candidial infection persistence in diabetes mellitus].

Population heterogeneity of Candida spp. in feces of patients with diabetes mellitus (DM) demonstrated in our earlier studies prompted to focus on the persistence of candidial infection depending on demographic characteristics of the patients and clinical features of DM. We examined 64 patients with DM and 40 practically healthy subjects aged 20-60 yr.

[Candida and Saccharomyces spp. fungal associations in fecal microbiocenosis of diabetes patients and healthy subjects].

The article is devoted to analysis of pathogenic and diagnostic significance of Candida and Saccharomyces co-existence in diabetic patients. These transient fungi are known to be present in fecal microbiocenosis of both healthy subjects and patients with diabetes mellitus. However, their overall occurrence is significantly increased in the disease and the structure of the biocenosis undergoes alteration.

[Quantitative assessment of in vitro neutralizing activity of secretory factors from rat lungs against influenza virus].

Secretory factors were isolated by lung wash followed by centrifugation to remove cells, dialysis of supernatant to remove NaCl salt, lyophilization of the lavage fluid and resuspention of the lyophilization product in an isotonic NaCl solution. It was shown that biological activity of influenza virus /Aichi/2/68 (3N2) significantly decreased (p = 0,01) from 8,17 +/- 0,10 to 7,14 +/- 0,20 IgEID50/ml during its incubation with secretory factors at 37 degrees C for 1 hr and to 7,92 +/- 0,17 IgEID50/ml in isotonic NaCl solution in the absence of these factors. Their concentration in the incubation medium was estimated to be 9.

[Molecular mechanisms of induction of innate immunity].

Cellular and molecular mechanisms of congenital immunity at different levels are discussed including single cell expression patterns and intracellular localization of individual TLR, the use of adapter molecules for generation of activation signals in response to microbial and non-microbial pathogens, soluble trap receptors, and intracellular negative regulators.

[Colonization of the rat gastrointestinal tract by Staphylococcus aureus biotopes].

Six groups of Wistar rats were fed different diets. Intestinal mural and fecal microflora was analysed. Four types of colonization of trointestinal tract were distinguished after intragastric administration of Staphylococcus aureus depending on a variety of factors affecting composition of microbial communities.

[Antimicrobial efficacy of semi-conductor laser with the wave length of 970 nm].

The 970 nm diode laser and 3% sodium hypochlorite antiseptic properties comparative estimation has been investigated. The investigated methods antimicrobial efficiency estimation has been made by means of microbiological study and by means of fluorescent laser analysis. It has been proved that laser radiation using allows the medicament treatment efficiency but doesn't assure provide total root canals sterility.

[Classification of lectins as universal regulator molecules of biological systems].

Modern concepts of lectin classification reflecting tendencies in the development of functional classification of lectins (Ln) and Ln-like proteins are presented. Classification of bacterial Ln is proposed. Classification of Ln and Ln-like proteins of plants, animals, and bacteria is based on 8 major elements of protein secondary structure that allows for the evaluation of topographic diversity of their surfaces, potential compatibility of bacterial, vegetable, and mammalian Ln molecules and possible variants of their co-functioning.

[Influence of various levels of vitamin D and calcium in the diet on variability of microbes association of rats].

It is established, that the microecological system of intestines microbes association structurally reacts to the maintenance of vitamin D and calcium in food. Thus change of frequency of occurrence, the specific maintenance and a dispersion of separate components of system is observed. Besides character and force of correlation communications between concentration of microbes varies.

[Modern methods of laboratory diagnostics of Chlamydia infections].

Need for further improvement of methods for verification of etiological agent of urogenital and respiratory chlamydiosis on the basis of increased biotechnological requirements to antigens for serological reactions, primers for PCR assay (refinement of connection of primers with microorganism's zones of genome most significant for its life activity or formation of most diagnostically significant complexes of primers), and selection of cultivating conditions considering the predicted features of clinical strains of the agent was substantiated.

[Nanotechnologies and perspectives of their application in medicine and biotechnology].

The results of nanotechnologies application in biological and medical fields are summarized. The examples of nanotechnologies use in reactions of cell adhesion, cell recognizing, intracellular sorting, organ and tissue construction, nanobiosensing, drug delivering and cancer therapy are presented. The prospects of nanotechnologies use in biomedicine, biothechnology and cell biology are shown.

[Influence of various dietary intakes on the rat's parietal microflora composition].

It was established that specific and quantitative characteristics of microecosystem of parietal mucin of rat's gastrointestinal tract, significantly varied depending on diet. Microbiocenosis of the colon was the most liable to alimental factor, but in the small intestine it was more depending on the endogenic factors of macroorganism. The principle of forming of microecosystem of parietal mucin of rat's gastrointestinal tract subject to composition of the diet was considered in the article.

[Opportunistic pathogenic microflora in intestine microbiocenosis of medical staff of multisectoral hospitals].

Microflora of colon in 81 medical staff was researched. Microecological disturbances mostly were represented by dysbacteriosis of second degree. The composition of intestinal microorganisms correlated with duration of the work in hospital.

[General properties and functioning principles of lectins in biological systems].

The authors give the literature review with analysis of main conceptions of lectins - their structure and functions according to state-of-the-art science and production requirements. Up-to-date definition of lectins based on last achievements of science and practice represented in the article.

[Strategic aspects of constructing probiotics of the future].

The publication systematizes modern achievements in understanding the role of microbiocenoses in the existence of macroorganism, and possible ways of constructing of probiotics of the future.

[Comparative analysis of the susceptibility and productivity of respiratory tract target cells of mice and rats exposed to inflienza virus in vitro].

The levels of susceptibility to influenza virus A/Aichi/2/68 H3N2 and the virus yield were determined using primary cells of the trachea and lungs of CD-1 mice and Wistar rats, and for 3 sets of cells obtained from primary lung cells of the both species by centrifugation in the gradient of density and by sedimentation on a surface. The values of ID50 virus dose for 10(6) cells and virus yield per 1 infected cell determined for primary mice cells were 4.0+/-0.