Formation of the central nervous system in ontogeny and function in adult mammals are controlled by universal ubiquitin-proteasome proteolytic system. The aim of this work was to study the dynamics of expression of immune proteasomes in comparison with the dynamics of ChLA and CLA proteasome and expression of the transcription factor Zif268 in the structures of the brain (cortex, hippocampus, and brainstem) in embryonic (E19, E21 days of embryonic development) and early postnatal (P1, P3, P4, P5, P7, P15 days of post-natal development) development in rats. ChLA and CLA in clarified homogenates of rat brain structures were determined by hydrolysis of fluorogenic commercial oligopeptides Suc-LLVY-AMC and Z-LLG-AMC, respectively.
View Article and Find Full Text PDFA novel approach to the development of a precise method of intraoperative diagnostics of thyroid cancer has been proposed on the basis of fundamental study of proteasomes in malignant tumors of mammals and human. The method is based on estimation of proteasome activity in small fragments of the tumor and adjacent tissues.
View Article and Find Full Text PDFNervous and immune systems have many general features in their organization and functioning in various animal species from insects to mammals. These systems are capable to regulate effectively each other by exchange of information through rather small molecules like oligopeptides, cytokines, and neuropeptides. For many such molecules, that function as transmitters or signaling peptides, their origin and receptors are common within nervous and immune systems.
View Article and Find Full Text PDFInduction of donor-specific tolerance in a recipient is one of the methods for enhancing acceptance of the grafts of endocrine glands in the absence of immunodepressants, which interfere with hormone production. This paper describes changes in the proteasome pool in the rat liver, spleen, and graft during the development of donor-specific tolerance after intraportally infusing the recipient with donor splenocytes with subsequent allografting of ovarian tissue into the renal capsule. It has been demonstrated that the shift in the balance in the liver and graft proteasome pools towards the variants with the LMP2 subunit determines the development of immunological tolerance and graft retention.
View Article and Find Full Text PDFThe object of the present work was detection of the genetic differences in the anxiety level evaluated in a number of behavioral tests and according to the characteristics of benzodiazepine receptors binding in the brain of rats of two inbred strains, WAG/G and Fischer-344. In all experimental situations ("conflict open field test", "mink chamber", "light-dark chamber", "Vogel's test", "elevated plus maze") Fischer-344 rats demonstrated a higher anxiety level than that of WAG/G rats. The concentration of benzodiazepine receptors in the brain was much higher in WAG/G than in Fischer-344 rats.
View Article and Find Full Text PDFThe effect of intranasal administration of bradykinin, ACTH(4-10) fragment, and vasopressin on the withdrawal syndrome was studied in morphine-dependent rats of two inbred strains, WAG/G and Fischer-344. Morphine dependence was stronger and the withdrawal syndrome had a more severe course in WAG/G rats. Intranasal administration of bradykinin and the ACTH(4-10) fragment in the dose used had no effect on the expression of the withdrawal syndrome in rats of both strains.
View Article and Find Full Text PDFA participation of nonopiate peptide brain systems in the mechanism of individual pain sensation was studied. The experiments were carried out on rats of two inbred strains, Fischer-344 and WAG/G, which exhibit different threshold of thermal pain sensation. The action of nonopiate oligopeptides (substance P, bradykinin, and thiroliberin) on the latent period of tail immersion from hot water was studied.
View Article and Find Full Text PDFBiull Eksp Biol Med
September 1995