[Antagonistic activity of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 and BIM B-439D strains towards pathogenic bacteria and micromycetes].

In this study the antagonistic activity of strains Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 and BIM B-439D against bacterial and fungal pathogens of agricultural crops has been researched. It is shown that both strains of bacilli demonstrated a high level of antagonism to the vascular bacteriosis pathogen, average level of antagonism to micromycetes--root rot pathogens.

[Dehydrogenases oxidizing ethanol and acetaldehide in Rhodococcus erythropolis EK-1].

Four types of alcohol- and acetaldehyde dehydrogenases were found in the cells of strain Rhodococcus erythropolis EK1 grown on ethanol. They are as follows: NAD-, NADP-, pyroquinoline quinone (PQQ)- and 4-nitroso-N,N-dimethyl aniline (NDMA)-dependent enzymes. Activity of NAD- and NADP+ -dependent alcohol dehydrogenases, as well as PQQ and NDMA-dependent acetaldehyde dehydrogenases was low and made up 3-11 nmol x min(-1) x mg(-1) of protein.

[Role of exogenic precursors in formation of surface-active substances during cultivation Rhodoococcus erythiropolis EK-1 on ethanol].

A possibility to intensify synthesis of surfactants of Rhodococcus erythropolis EK-1 under the presence of citrate (lipid synthesis regulator) and fumarate (gluconeogenesis precursor) has been shown. A 40-100% increase of indices of surfactants synthesis with introduction of cytrate (0.1%) and fumerate (0.

[Effect of cultivation conditions on the physicochemical properties of exopolysaccharide ethapolan].

The physicochemical properties of the complex exopolysaccharide ethapolan (EPS) produced by Acinetobacter sp. 12S during growth on media with various C/N ratios and different concentrations of mineral components and phosphate buffer were studied. Irrespective of the cultivation conditions, the concentrations of carbohydrates (38-44%) and pyruvic acid (3.

[C2 metabolism and intensification of the synthesis of surface-active substances in Rhodococcus erythropolis EK-1 grown on ethanol].

Oxidation of ethanol, acetaldehyde, and acetate in Rhodococcus erythropolis EK-1, producer of surface-active substances (SAS), is catalyzed by N,N-dimethyl-4-nitrosoaniline (DMNA)-dependent alcohol dehydrogenase, NAD+/NADP(+)-dependent dehydrogenases (optimum pH 9.5), and acetate kinase/acetyl-CoA-synthetase, respectively. The glyoxylate cycle and complete tricarboxylic acid cycle function in the cells of R.

[Formation of the exopolysaccharide ethapolan by Acinetobacter sp. IMV B-7005 on a fumarate-glucose mixture].

Our studies enabled us to intensify the synthesis of the microbial exopolysaccharide (EPS) ethapolan produced by Acinetobacter sp. IMV B-7005 grown on a mixture of fumarate (an energy-excessive substrate) and glucose (an energy-deficient substrate). Supplementing glucose-containing medium with sodium (potassium) fumarate at a molar ratio of 4:1 resulted in a 1.

[Metabolism characteristics of Acinetobacter sp. IMS B-7005 grown on the mixture of C2,-C6-substrates].

It has been established that under the conditions of mixotrophic growth of Acinetobacter sp. B-7005 on the mixture of sodium acetate and glucose the activity of acetyl-KoA-synthetase and that of phosphoenolpyruvate synthetase were 10 times lower and indices of synthesis of microbial exopolysaccharide (EPS) ethapolan are almost 2 times lower than on the mixture of potassium acetate and glucose. It is supposed that the positive role of sodium cations may consist in their participation in the active transport of acetate to the cells of Acinetobacter sp.

[Synthesis of the exopolysaccharide ethapolan on a mixture of energy-deficient growth substrates].

Intensification of the synthesis of the microbial exopolysaccharide ethapolan by Acinetobacter sp. B-7005 was shown to occur on a mixture of energy-deficient growth substrates (acetate + glucose). When the bacterium grew on the substrate mixture, both substrates were utilized simultaneously; acetate was taken up by means of active transport at the expense of the energy of the proton-motive force.

[Effect of acetate on synthesis of exopolysaccharide ethapolan during Acinetobacter sp. B-7005 cultivation on the medium with ethanol].

A possibility of removal of C2-metabolism limiting and increasing of activity of acetyl-CoA-synthesis in Acinetobacter sp. B-7005--producer of exopolysaccharide (EPS) ethapolan with acetate introduction in the medium with ethanol. When adding potassium acetate 0.

[Synthesis of microbial exopolysaccharide ethapolan on ethanol and molasses mix].

A possibility to change glucose, when cultivating exopolysaccharide (EPS) producer etapolan Acinetobacter sp. B-7005 on a mix of C2-C6-compounds, by the inexpensive substrate--molasses has been shown. The highest indices of EPS synthesis were observed under the conditions of preliminary hydrolysis of molasses, availability of growth factors (yeast autholisate and calcium pantothenate) in the medium, lack of the mineral source of nitrogen nutrition and use of inoculation material grown on acetate.

[Regulation of C2-metabolism under mixotrofic growth conditions of Acinetobacter sp. B-7005 on ethanol and glucose mixture].

The possibility of elimination of C2-metabolism limitation under Acinetobacter sp. B-7005 (producer of exopolysaccharide ethapolan) cultivation on ethanol-glucose mixture was shown. Cultivation of these bacteria on the nonbuffered medium was accompanied by pH decrease (5.