[The effect of Yersinia pestis antigens and strains on the level of lymphocyte blast transformation].

Lymphocytic blast-cell transformation (LBCT) is shown to be related both to the genotype of a macroorganism and to the genetically determined expression of individual antigens by the plague microbe. The correlation of LBCT with the level of antiinfectious resistance suggests that this index is of high informative value and that it can be effectively used for testing the cellular immunity and the characteristically biological properties of the plague causative agent and its antigens.

[Interleukin-1 induction by different antigens and strains of Yersinia pestis bacteria in immunized mice].

In this work the influence of Y. pestis antigens (capsular antigen, mouse toxin, lipopolysaccharide, pesticin), as well as the products of gene expression localized on three Y. pestis resident plasmids, have been studied.

[Glycine-dependent cryotransformation of Bacillus anthracis by DNA of various plasmids].

The possibility of the 1,8-60,0 Md plasmids DNA transfer into the cells of different Bacillus anthracis strains has been established. A number of parameters of the procedure has been optimized. The including of PEG6000 into the cryomixture in the final concentration 10% has been demonstrated to be vital for increase in transformants yields.

[Transduction and conjugation transfer of the pXO2 plasmid in Bacillus anthracis].

The plasmid pXO2 determining the capsule synthesis has been shown to be transfered into the cells of different strains of Bacillus anthracis (STI-1, Sterne, KM33, KM35) by the transducing bacteriophage CP54ant and by mobilization by pAM beta 1 replicon with the frequencies, consequently, n.10(-8) and n.10(-7).