The study aimed to investigate how cytokine gene expression changes during the cultivation of leukocytes from healthy donors.
RNA from leukocytes was analyzed at different time points (0, 3, and 24 hours), focusing on 8 cytokine genes using PCR techniques.
Results showed that after 3 hours, gene expression for pro-inflammatory cytokines peaked, while expression levels of anti-inflammatory cytokines remained stable over 24 hours.