[The environmental and epidemiological aspects of malaria in Armenia and prospects for its eradication].

The malaria epidemiological situation in Armenia has begun deteriorating since 1994 when the increased importation of vivax malaria made the previously endemic foci of malaria to function. In 1998, the maximum cases (n = 1156) were notified; of them, there were 542 cases of local origin. The main vectors of malaria in Armenia were An.

[The species composition of mosquitoes and ticks in Armenia].

Comprehensive epidemiological, entomological, virological, and parasitological studies were conducted to examine the species composition and size of bloodsucking arthropoda (mosquitoes and ticks). A total of 64,567 mosquitoes and 45,180 Ixodes ticks were collected. Among the mosquitoes, Anopheles maculipennis was a prevalent species (81.

[Seroepidemiological characterization of virus hepatitis in the Republic of Armenia].

The survey of the population immunological structure with respect to parenteral hepatitis showed awide circulation of hepatitis B (HB) and hepatitis C (HC) viruses among the adult population of Armenia. During the 5 year period of observation the number of persons having antibodies to HC virus increased 2.7-fold.

[Antibiotic properties of Lactobacillus acidophilus, "Narine" strain, and the ways for their improvement].

L. acidophilus strain 317/402 "Nariné" shows high antimicrobial activity with respect to pathogenic microorganisms. The degree of the activity of lactobacilli depends on cultivation conditions and the composition of the culture medium and its acidity, age of the culture, conditions of technological process, etc.

[Immunological features of patients with polypous ethmoiditis].

34 patients with nasal polyps (NP) and 12 normal individuals were studied immunologically to investigate association of nasal polyp formation with disorders of general and local immunity. In NP patients there were decreased preoperative and early postoperative levels of peripheral blood T- and B-lymphocytes, functional activity of lymphocytes and neutrophils. In contrast to normal individuals, nasal secretion of NP patients contained degenerative epithelial cells and neutrophils, activated lymphocytes, monocytes and eosinophils.

[The nuclease activity of the cell nuclei in 2 lines of murine myeloma sp2/0 differing in their resistance to cytostatics in adriamycin-induced apoptosis].

The endonuclease activity of two drug-sensitive and drug-resistant mouse myeloma cell lines during cytotoxic drug-induced apoptosis was studied. It was shown that internucleosomal fragmentation of DNA in drug-sensitive line sp2/0, undergoing apoptosis in the presence of adriamycin and colchicine, was not dependent on intracellular calcium content and was associated with activation of both Ca(2+)-Mg(2+)-dependent and acidic cation-independent endonucleases. In contrast, in multidrug resistant spEBR-5 cells, treated with the same drugs, only Ca(2+)-Mg(2+)-dependent endonuclease activity was detected.

[The effect of adriamycin and ethidium bromide on the Ca2(+)-dependent K channel of human erythrocytes].

The influence of cytotoxic agents adriamycine (AD) and ethydium bromide (EB) on Ca(2+)-dependent K+ channels of erythrocytes was investigated. The incubation of erythrocytes with agliconic part of AD (without aminosugar residue) increased Ca(2+)-dependent K+ efflux induced by low concentration of propranolol, while EB suppressed the activating effect of propranolol. EB, verapamil and triphluoroperazine inhibited Ca(2+)-dependent K+ efflux induced by high doses of propranolol.

[The interaction of human natural killers with target cells of the K562 line and its sublines characterized by multiple drug resistance and thermoresistance].

Target cells, K562 strain and its sublines characterized by multiple drug resistance (MDR) do not differ in their susceptibility to human natural killer cells (NK) but MDR cells are more susceptible to cytotoxic action of lymphokine-activated cells (LAC) and to NK cells in the presence of a selective agent adriamycin. Target cells death is characterized by fragmentation of nuclear DNA. It has been established that K562 thermotolerant subclone is more resistant to NK and LAC than other clones.

[The adaptive response of B cells in culture to the action of cytotoxic agents. I. The stimulation of immunoglobulin synthesis and secretion by myeloma cells, hybridomas and human lymphocytes under the influence of adriamycin and ethidium bromide].

Effects of cytotoxic drugs, doxorubicin (adriamycin) and ethidium bromide, on immunoglobulin production have been studied in B-cell hybridomas, myelomas and human lymphocytes. Doxorubicin, and in a lesser extent ethidium bromide, are shown to enhance immunoglobulin production both in drug-resistant and drug-sensitive cell lines. Enhancement of immunoglobulin production is accompanied with that of the number of antibody forming cells and with immunoglobulin synthesis in these cells.

[The adaptive response of B cells to the action of cytotoxic preparations in culture. III. The possible mechanism of the action of adriamycin on immunoglobin synthesis by the cells of murine myeloma].

A dose-dependent stimulation of immunoglobulin synthesis by Spebr-5 cells in the presence of adriamycin is shown. This effect is significantly blocked in the presence of iron-chelating agent 2,2-bipyridine. Moreover, low doses of H2O2 and heat shock also caused enhancement of immunoglobulin synthesis.

[The adaptive response of B cells to the action of cytotoxic preparations in culture. II. Immunoglobulin interaction with adriamycin and ethidium bromide].

The binding of adriamycin and ethidium bromide coupled to sepharose beads with intracellular and serum immunoglobulins is detected. These drugs do not bind with Fab and Fc fragments of immunoglobulin. The binding of ethidium bromide to immunoglobulins is competitive.

[Ability of the cells of intra- and interspecific hybrids of murine hepatoma XXIIa to synthesize the serum proteins albumin and transferrin].

Independent hybrid clones resulted from the whole cell and microcell-mediated transfer of hamster or mouse fibroblast chromosomes into mouse hepatoma XXIIa cells. The fusion was promoted with PEG, ethidium bromide alone, or in combination with HAT and ouabain, was used for selecting the hybrids. Using indirect immunoautoradiography, three clones (one intra- and one interspecies microcellular; one interspecies, whole cell fusion) have been found to express their hepatic function to synthesize transferrin.

[Characteristics of the antigenic composition of the cells of intra- and interspecific hybrids of murine hepatoma XXIIa].

Hybrids of cultivated mouse hepatoma XXIIA cells were studied for the presence of species-specificity antigens. Forssman antigen, and antigen similar to human B-isoantigen. The cells of MGXXIIA hepatoma, clone culture of L mouse fibroblasters and RJK hamster cells were used as parental cells.

[Synthesis of alpha-fetoprotein, albumin and transferrin by long-term cultured cells of murine hepatoma].

The capacity of continuous cell of XXIIa mouse hepatoma (strain MHXXIIa) to synthesize alpha-fetoprotein, albumin and transferrin was studied by immunoautoradiography. Albumin and transferrin were detected in the polyethylene glycol concentrated growth medium of hepatoma cells on the 5th year (the 55th month) of their cultivation. alpha-fetoprotein was not found.