[Development of a rapid method for the detection of prostate-specific antigen by immunochromatography].

A single-step qualitative rapid test for the determination of prostate-specific antigen (PSA) in samples of human blood serum by immunochromatography using a complex of colloidal gold with monoclonal antibodies to PSA as the detection agent was developed. The determination limit for PSA in serum blood samples is 10 ng/ml; the analysis time, 15-25 min; the sensitivity of the method, 100%; and its specificity, 92.5%.

[Markers of asbestos exposure in workers].

Examination covered 213 workers of asbestos technical ware plant. Standard X-ray examination no pulmonary and pleural changes were revealed. External respiration studies demonstrated moderate ventilation disorders in half of the examinees: in hardware workshop workers--restrictive type disorders, in autofriction components workshop workers--obstructive type ones with hyperventilation.

[Leptin levels in patients with bronchial asthma and diabetes mellitus].

Aim: To estimate leptin production in long-term steroid therapy, insulin resistance and obesity.

Material And Methods: Leptin concentrations were measured with enzyme immunoassay in 101 patients with bronchial asthma and diabetes mellitus and 31 healthy controls with overweight. The mean age was 54.

[Advantages of periodic medical examinations of asbestos plant workers at the Occupational Center].

The article deals with results of periodic medical examination conducted in Regional Occupational Center MONICI for 90 subjects working in apparatus workshop of asbestos ware plant. Data presented are results of thorough evaluation of respiration biomechanics through electronic spirometry and body plethysmography, serum activity of interstitial alveolomucin 3EG5 and oncologic markers CA-125 and CA-15.3.

[Levels of mucin-antigen 3EG5 in the blood and bronchoalveolar swabs in patients with tuberculosis, sarcoidosis and fibrosing alveolitis].

The levels of the antigen mucin 3EG5, a marker of the activity of interstitial lung diseases, were studied in the sera and bronchoalveolar swabs of patients with active tuberculosis, sarcoidosis of the respiratory organs, and idiopathic and exogenous allergic alveolitis. Its higher blood levels were detectable in patients with alveolitis (exogenous allergic ones to a greater extent) and absent in active tuberculosis and sarcoidosis. There was a correlation of the levels of the antigen mucin 3EG5 in the blood and bronchoalveolar swabs with the degree of lymphocytosis in the endopulmonal cytogram.

[Effectiveness of antiinflammatory therapy in patients with interstitial lung diseases using re-assay of biomarker mucin 3EG5].

Aim: To investigate a potential of a new marker of activity of interstitial pulmonary diseases (IPD)--mucin 3EG5--in monitoring of effectiveness of the antiinflammatory therapy.

Materials And Methods: Serum mucin antigen 3EG5 (glycolyl-sialylated glycoprotein) was assessed with sandwich immunoassay constructed using a monoclonal antibody 3E1.2 (Medical Innovations, Australia) and affinity-purified polyclonal antibody G5 (Xema, Russia) against total mucin antigen family.

[Pulmonary aspergillosis in patients with disseminated processes in the lungs].

Sera of 30 patients with disseminated processes in the lungs of various etiology were examined for the presence of specific IgG to antigens of Aspergillus fumigatus (A.f.) and Candida albicans (C.

[Development and use of a method of determination of specific immunoglobulin G based on immunoenzyme analysis in the diagnosis of pulmonary aspergillosis].

Enzyme-linked immunoassay (ELISA) was employed for determination of specific immunoglobulin G to Aspergillus fumigatus (A.f.).

[Immunoglobulin E in ankylosing spondylarthritis].

Ankylosing spondylarthritis (AS) patients (n-95) were examined for IgE concentrations using radioimmunoassay (commercial kits "Pharmacia"). Elevated IgE concentrations (greater than 100 Ku/l) were recorded in 16% out of 45 patients with central AS and in 40% out of 50 patients with peripheral AS (p less than 0.05).

[A new Soviet allergen made from bee venom for the specific diagnosis of allergic reactions to bee stings].

Overall 46 patients with allergic reactions to bee stings were examined. As a result of making skin prick, scarification and intracutaneous tests with an allergen from bee venom, allergy to bee stings was revealed in all the 46 patients, whereas only 37 patients responded to an allergen from the bee body. Specific IgE-antibodies using RAST were detected in 29 out of 31 patients.

[The use of monoclonal antibodies with human IgE for determining the level of allergen-specific IgE].

The possibility of using monoclonal antibodies to human IgE, obtained in our laboratory, with the aim to determine allergen-specific IgE in the radioallergosorbent test (RAST), the enzyme immunoassay and the radioimmunoassay has been studied. The results obtained with the use of the standard RAST system (manufactured by Pharmacia, Sweden) and different variants of assay based on the use of our antibodies, labeled with 125I or conjugated with peroxidase, and timothy pollen allergen (obtained from the Stavropol Research Institute for Vaccines and Sera) have been compared. The best results have been obtained with anti-IgE antibodies 11-2, but for working out our assay systems it is necessary to develop our own standards and control limits.

[The treatment of infectious-allergic bronchial asthma by the extracorporeal connection of a donor spleen].

A total of 25 patients suffering from infective allergic bronchial asthma underwent extracorporeal connection of the donor's spleen (ECCDS). Before ECCDS was made the health status was unstable in all the patients, with asphyxia attacks occurring fairly often, namely up to 12 times a day. Nine patients received the maintenance therapy with steroid hormones.

[The clinical use of immunosorption: problems and perspectives].

The data concerning clinical use of extracorporeal immunoadsorption are summarized. At present, broad use of this therapeutic approach is hampered by several technological and medical problems such as incomplete biocompatibility of carrier, pyrogenic reactions, ligand "leakage" and rebound phenomena. However, the technology of ex vivo removal is much more developed, than the fundamental knowledge of relevant target substance to be removed in specific clinical situation.

[Clinical immunosorbents on a basis of polymeric matrices].

Immobilization of anti-IgE on space-network polymers containing aliphatic amino- and hydrazido groups as a way of producing clinical immunosorbents has been studied. Influence of active group concentration on the specific activity of the immobilized antibodies and sorption dynamics of IgE from plasma of patents are investigated. Immunosorbents can be sterilized by gamma-irradiation without any loss of capacity.