[Influence of pH values on the agglutinating capacity of anti-A-monoclonal antibodies and their inhibition of A-glucoconjugates of the lipid and protein nature with various isoelectric properties].

The effect caused by medium acidification up to pH 6.5 on the agglutinating ability of 7 anti-A MAbs (2-8, 2-17, 2-19, 2-22, 2-23, 2-28 from Workshop IV and BRIC-145) and their inhibition by glycoconjugates obtained from the membranes of A1 erythrocytes by enzymatic treatment and the chloroform-methanol method, followed by ion-exchange gel chromatography, was evaluated. Medium acidification most significantly reduced the agglutination of A1 erythrocytes in IgM MAbs 2-17 , 2-19, 2-22, and 2-28 and had a weaker manifestation in more alkaline IgM MAbs 2-8, 2-23, and BRIC-145.

[The substantination of isotypical differentiation in AB0 system against nonagglutinogenic acid glycotopes of lipid origin].

Monoclonal reagents from Workshop IV were inhibited by glucoconjugates obtained from the membranes of 3 samples of AB (erythrocytes of different isotypes) by enzymatic treatment and the chloroform-methanol method and tested both serologically and in cell electrophoresis by a change of electrophoretic motility under the influence of antibodies and the complement. Glycoconjugates of the lipid and protein origin were additionally subjected to separation by the ion-exchange column chromatography on fractions of the alkaline and acid types. The differences developed in the inhibiting ability of the acid fractions of A and B antigens.

[Comparison of the antigenic spectrum A1, A2, and Ax erythrocytes: inhibition of mab with glucoconjugates of lipid and protein nature with different isoelectric properties].

The inhibition of monoclonal antibodies (MAbs) (anti-A1 2-24, anti-A 2-22, anti- 2-19, and anti- A 2-18; Workshop IV) by glycotopes (glycoconjugates) obtained by the enzymatic treatment of A1, A2, and Ax erythrocytes and by chloroform-methanol method from the membranes of these erythrocytes, followed by ion-exchange gel chromatography was evaluated. According to specificity and isoelectric properties, glycoconjugates (in the sequence of desorption from an anion exchanger) of glycoprotein (A(pr-00), A(pr-0), A(pr-1), A(pr-3)) and glycosphingolipid (A1p,00, A(1p-0), A(1p-1), A(1p-3), A(1p-3)) origin were identified. A1 erythrocytes showed the broad-spectrum inhibiting activity of glycoconjugates, which being at maximum with acid A(1p-00) and A(pr-3) (the ionic points of pH 6.

[Study of the nature of A1- and A2-antigenic differences with use of monoclonal antibodies: a role of glycoprotein and glycolipid epitopes in their formation].

The author used of A1- and A2-red blood cells to absorb Workshop-IV monoclonal reagents (anti-A1 2-24, 2-25, 2-27; anti-A 2-4, 2-10, 2-28; anti-H 2-71, 2-74) and anti-AHP protection, inhibited them with protein glycoconjugates (Apr), obtained by treating red blood cells with trypsin at pH 6.6 and by separating the supernatant on anion-exchange columns, and with lipid glycoconjugates (Alp), obtained by isolating glycosphingolipids from the trypsin-pretreated red blood cell membranes by the chloroform-methanol method and by subsequently performing anion-exchange gel chromatography. Judging from the results of the absorption using A1- and A2-red blood cells, the antigenic spectrum of A1 is more complete and exceeds that of A2, which permitted the use of the uniform test system of glycoconjugates from A1-red blood cells.

[Immunomodulation in dystrophic diseases of the joints].

Immunological examinations of 357 patients with mixed arthritis, coxarthrosis and aseptic necrosis of the femoral head have revealed substantial immunopathological deviations requiring immunomodulation and influencing surgical outcomes, with inhibition or dysfunction of T and B cellular reactions, inversion of the regulatory index, remarkable autoimmune component, and bacterial sensitization in a considerable part of the examined. In addition to the conventional immunomodulating agents (levamisole, thymalin, T-activin, sodium nucleinate), it is recommended that hemodes and polyglucin may be used, provided they are chosen individually in vitro. The use of ELISA made it possible to reveal a direct strong correlation between the level of antibodies to glycolipid cartilaginous antigen and arteparon as well as a decrease of the number of antibodies in therapeutic administration of the drug.

[Therapeutic approach in degenerative-dystrophic diseases of the hip joint in relation to the immunologic status].

In the process of study of the immunological status peculiarities of 202 patients with some degenerative-dystrophic diseases of hip joint has been revealed that the hyperimmune reactions to tissular antigens of the joint components (bone, cartilage, synovial membrane) are more often observed in case of non-specific arthritis, asephic necrosis of the head of the femur and dysplastic coxarthrosis. These were precisely the states with more frequent postoperative complications, provoking the process development, joint ankylosing or formation of ossificates. Preoperative immunocorrection therapy of 20 patients by means of a course of low-dose injections of plasma substitutes (polyglukine, hemodesis or reopolyglukine), individually selected by RIML and lymphocytotoxic test, allowed to arrest the lryperimmune manifestations.

[Contraindications regarding total prosthesis of the hip joint].

The authors have made an analysis of unsatisfactory remote results in 83 (21.5%) out of 387 patients operated for endoprosthezing of the hip joint which resulted from shaking loose the endoprostheses in the bone tissue. A conclusion is made that the following factors may be considered as contraindications to endoprosthesis of the hip joints: coxarthrosis against the background of arthroso-arthritis, neglected stages of protrusive coxarthrites, deforming osteoarthrosis due to the use of corticosteroids, pronounced senile osteoporosis.

[Chemoluminescence of preserved bone tissue].

The luminescence of bone tissue was studied during its treatment at preservation. It was found that vacuum drying as well as bone saturation with ascorbic acid and some antibiotics (streptomycin, monomycin) depressed the luminescence emission. Combined influence of these factors was found to be more difficult than for each one separately.