[Rotenone-sensitive oxidation of NADH and F0F1-ATPase activity in a homogenate of rat skeletal muscles during thermal adaptation].

A method has been developed for measuring the rates of rotenone-sensitive oxidation of NADH and oligomycin-sensitive hydrolysis of ATP in rat skeletal muscle homogenates. The method is based on the use of alamethicin which increases the permeability of the inner mitochondrial membrane for NADH and ATP. It has been shown that prolonged cold adaptation of rats (4 weeks, 4 degrees) does not change the activity of rotenone-sensitive NADH-oxidase in rat skeletal muscle homogenates which is equal to 12.

[Status of liver mitochondria and rat tissue creatine kinase during administration of antivitamin K].

It has been shown that 16-18 days administration of antivitamin K (pelentan) leads to two-fold increase of prothrombin time in adult rats but does not influence the soluble brain, renal, heart, muscle and serum creatine kinase activity. No effect on metabolic function of isolated liver mitochondria has been found in contrast to the vitamin K deficient rats. Mitochondria were characterized by high value of respiration control; substance oxidation rates and internal mitochondrial Ca2+ content do not differ in the level from those of control animals.

[Metabolic function of isolated liver mitochondria during various conditions of vitamin D and K supply and administration of pelentane].

Alimentary deficiency of vitamin K caused a decrease in the rate of respiration in presence of ADP and in the rate of oxidative phosphorylation in the presence of succinate. Administration of the antivitamin K pelentane, excess of vikasol and deficiency of vitamin D did not affect these parameters. As distinct from controls and rats treated with pelentane, transport of calcium was decreased in presence of all the substrates studied in mitochondria isolated from liver tissue of animals deprived of vitamins K and D as well as of animals treated with vikasol excess.

[Nature of endogenous proton conductance of the inner mitochondrial membrane. Role of Ca2+ transport system in proton transfer].

In order to elucidate the nature of endogenous proton conductance of rat liver inner mitochondrial membrane, the dependence of the rate of Ca2+ transport on pH was studied. It was found that the inhibiting effect of H+ is independent of protonation of functional groups of hypothetical Ca2+ carrier, but results from electrogenic transfer of H+ across the membrane, which is highly permeable for the proton. The adsorption of H+ by mitochondria is inhibited by ruthenium red and other specific inhibitors of Ca2+ transport.

[Role of planar cobalt chelate complexes as shunting agents in the electron transport chain of mitochondria].

It was shown that planar cobalt chelate complexes catalyze oxidation of NADH and hexahydroubiquinole Q4H2 by air oxygen. The most active agent--perchlorate of the cobalt complex 1, 2, 3, 7, 8, 12, 13, 17, 18, 19-decamethyloctadehydrocorrin--is capable of shunting the electron transfer chain of rat liver mitochondria and beef heart submitochondrial particles, catalyzing electron transfer from CoQ directly to the oxygen.