[Selective accumulation of 131I-labeled IKO-1 monoclonal antibodies in the tissues of mouse L 1210 lymphoid leukemia].

Monoclonal antibodies (MCA) ICO-1 of IgG3 isotype against 1a-like human antigens were labelled with 131I using chloramine T; a radionuclide-bound fraction was isolated by radiochromatography. Basing on the results of assessment of complement-dependent cytotoxicity against lymphocytes from the lymph nodes of BDF1 mice a conclusion was made that MCA immunological reactivity changed slightly after radionuclide tracer administration. In vitro experiments with a panel of normal and malignant cells showed 131I-MCA binding with cells of mouse lymphoid leukemia L1210 and hemocytoblastosis La as well as with spleen and lymph node normal lymphocytes.

[Disorders of immunologic homeostasis as affected by radioactive iodine preparations].

(CBA X C57B1/6)F1 mice were injected with 125I and 131I-sodium iodide at a dose of 5.74 X 10(4) Bq/g. For a long time after such treatment the animals manifested an increased level of spleen cells humoral immune response to a foreign antigen (sheep erythrocytes), the autoreactivity of spleen and lymph node lymphocytes to autologous erythrocytes was also elevated.

[Functional activity of cells suppressing the humoral immune response in internal irradiation].

In the adoptive transfer system of (CBA X C57BL/6)F1 mice, the estimation was made of the function of splenic cells, suppressors of the humoral immune response to sheep erythrocytes 1 and 6 months following the injection of 125I and 131I. Low absorbed doses of the radioactive isotopes were shown to stimulate the activity of the suppressors generated in mouse spleen.

[Lymphocyte migration in internally irradiated animals. Effect of internal beta radiation on the migration of spleen lymphocytes in CBA mice].

The syngeneic transfer system was used to study migration of 51Cr-labelled spleen lymphocytes in mice after incorporation of beta-emitter, 35S-methionine. Migration of 51Cr-labelled lymphocytes to lymph nodes was stably decreased, and to liver, kidneys and lungs increased. The lymphocyte migration impairment was associated with the influence of beta-radiation on both the migratory properties of cells and the factors of their microenvironment responsible for the lymphocyte migration within the mouse body.

[Lymphocyte migration in internally irradiated animals. Effect of internal gamma radiation on the migration properties of spleen lymphocytes labeled with 51Cr].

In experiments on CBA mice it was shown that migration of 51Cr-labeled spleen lymphocytes, injected intravenously, to lymph nodes of intact recipients was suppressed 6-24 months after the administration of a radiopharmaceutic preparation of selenium-75-selenomethionine in a quantity forming the doses of 1 Gy and 1.5 Gy absorbed within the whole body and lymphoid organs, respectively. Migration of labeled lymphocytes to the liver, kidneys and lungs, as well as their retention in the circulating blood, were increased.

[Lymphocyte migration in the body in conditions of internal irradiation. 2. Disorders of lymphocyte microenvironmental factors in organs].

At later times after the injection of selenium-75-selenium methionine to CBA mice the inhibition of migration of intravenously injected normal 51Cr lymphocytes to the lymph nodes and liver lasted for a long time. On the contrary, no changes in the normal lymphocyte migration within the animal body were observed after total-body gamma-irradiation with the dose of 4 Gy. The data obtained are indicative of the radiation-induced disturbance of the microenvironment factors, which provide migration of lymphocytes within the body, during the long-term exposure to radiation emitted by the incorporated source.

[Age-related changes in splenic lymphocyte migration in mice].

Age changes of distribution of the labelled mouse lymphocytes in the organs of syngeneic recipients were followed. The changes were shown to concern both the circulating lymphocytes and their environment, the latter having suffered greater changes. On the contrary, changes in the lymphocytes played the leading role in this process in the autoimmune mice NZB.