[Invasive streptococcal (group A) infection of soft tissues in a Moscow surgical hospital].

Aim: To study features and risk factors of soft tissue's invasive streptococcal infection in surgical unit's patients: a) emm-types of the most often recovered streptococcus; b) human immune response for streptococcal antigens (streptolysin-O and the group A carbohydrate antigen); c) main patient's conditions that complicate the course of soft tissue's streptococcal infection.

Patients And Methods: 101 surgical unit's patients with soft tissue's infection, which group A streptococcal (GAS) nature, were examined. Emm-types were determined by comparison ofemm gene sequenced part with data of CDC Streptococcus Laboratory.

[Humoral immune response in patients with angina and soft tissue infections of group a streptococci etiology].

Evaluate informativity of simultaneous determination of antibodies (AB) against extracellular (AB against streptolysin-O-ASL-O) and cellular (IgM against A-polysaccharide - A-PSC) antigens in patients with angina and soft tissue infections caused by serogroup A streptococci (SGA) and identify features of humoral immune response to SGA infection according to infectious process localization. MATERIALS AND METHODS. 2 groups of patients with bacteriologically confirmed SGA infection (50 cases of angina - group 1 and 51 case of soft tissue infection - group 2) were examined for the presence of ASL-O by using Architect ci8200 analyzer (Abbott, USA) and IgM against SGA A-PSC by EIA.

[Development of Streptococcus pyogenes cells inactivation for turbidimetric determination of bacteriolytic activity of phage-associated enzyme].

Aim: Development a method of treatment of Streptococcus pyogenes bacteria that does not disrupt the integrity of surface structure of cell and provides optimal reproducibility of enzyme preparation activity test results.

Materials And Methods: Museum cultures of S. pyogenes M29 and S.

[New technologies in the diagnosis and treatment of patients with squamous cell carcinoma of the anal canal].

Purpose Of The Study: The development of the rectum preservation treatment for the squamous-cell carcinoma of the anal canal.

Materials And Methods: The following scheme of the rectum preservation treatment for the squamous-cell carcinoma of the anal canal was developed and has been used in the Cancer Research Center RAMS since 1995. The irradiation is performed to the small pelvis, perineum and ilioinguinal lymph nodes, at a single dose 2 Gy, 22 sessions, total dose 44 Gy.

[Persistence of Streptococcus pyogenes].

State-of-the-art data on persistence of Streptococcus pyogenes are presented. Survival and persistence of S. pyogenes in organism of the host are considered as a result of action of widely presented virulent functions of infectious agent aimed on evasion from defense mechanisms of the host.

[Genetic characteristic of toxigenicity of group A streptococci cultures isolated from patients with tonsillitis].

Twenty-eight strains of group A streptococci (GAS) isolated from patients with lacunar and fibrinous-necrotic forms of tonsillitis during its 1st or recurrent episode were tested for presence of genes of erythrogenictoxins A, B, C, and Fusing PCR assay. Obtained results allow to consider that clinical features of the disease (severity, repeatedness, clinical form) can be determined by toxigenicity of GAS. Express identification of S.

[Lipoteichoic and teichoic acids of pathogenic streptococci: structure, functions, and role in interaction of the infectious agent with organism].

Features of structure, localization, and function of teichoic (TA) and lipoteichoic acids (LPA) of gram-positive bacteria are shortly characterized. Structures of polymers obtained from Streptococcus pyogenes, S. pneumoniae, S.

[Genotypic emm-typing of streptococcal cultures, group A, isolated from patients with different clinical manifestations of streptococcal infection in Moscow].

The results of the intraspecific typing of group A streptococci (SGA), isolated from patients with different manifestations of group A streptococcal infection, carried out in Russia for the first time, are presented. The genotypic method of emm-typing, based on sequencing the DNA area coding the variable part of the molecule of SGA M-protein was used. The data obtained in this study made it possible to follow changes in the pattern of SGA M-types in Moscow and the specific features of SGA in comparison with the analogous data on other territories.

[Ileotransversoanastomosis end-to-end in right-sided hemicolectomy].

The results of surgical treatment of 23 patients with cancer of right hemicolon with ileotransversotomy end-to-end are analysed. Advantages of this anastomosis in comparison with anastomosis "end-to-side" and "side-to-side" are shown. There were neither cases of insufficiency of sutures nor functional disturbances at the area of anastomosis in 23 operations.

[Inguinal lymphadenectomy in malignant anal tumors].

The evidence on the immediate and end-results of lymphadenectomy carried out in 36 patients with malignant tumors of the anal canal is presented. It appears to be expeditious to opt, for inguinal lymphadenectomy (after Ducuing) in cases of involvement of the inguinal lymph nodes suffering from primary malignant tumors of the anal canal.

[Lower anterior (mesenteric) resection of the rectum].

The immediate and late results of 283 operations for anterior resection of the rectum in carcinoma are analysed. Low anterior resection of the rectum with division of the lateral ligaments and middle rectal arteries was performed in 28 (9.9 +/- 1.

[Use of thermal radiochemotherapy in patients with anal epidermoid cancer].

The authors have developed a new original procedure for combined and multimodal treatment of patients with anal dermoid cancer, which includes radiation therapy, polychemotherapy (platidiam and bleomycin) by using topical superhigh-frequency hyperthermia and antioxidants. The treatment regimen proposed has turned out to be highly effective. It allows sphincter-preserving therapy to be performed without rectal extirpation in most primary patients with anal dermoid cancer.

[The early withdrawal of antibiotics in the postoperative period as the cause of the occurrence of suppurative inflammatory complications in oncological proctology].

The problems of antibiotics use duration after operations on the colon are discussed in the article. The authors supposes the early antibiotics abolition inexpedient because of serious complications appearance possibility. Even during the noncomplicated postoperative course the optimal antibiotic therapy duration is 7-10 days long.

[Malignant tumors of the anal canal].

Results of treatment of 381 cases of various malignancies of the anal canal were analysed. The above tumors were classified according to clinical course, histology and stage. Various treatment modalities, peculiarities of early diagnosis, causes of tumor advancement and end results of treatment of cancer of the anal canal are discussed.

[The treatment of squamous cell cancer of the anal canal].

Three methods of treatment for squamous-cell cancer of the anal canal, namely abdominoperineal extirpation of the rectum, radiotherapy and thermoradiotherapy were compared. The rate of relapse following thermoradiotherapy was 5.4 times lower than in the radiotherapy alone group and 3.

[Use of Y-shaped anastomosis in complicated cancer of the left half of the colon].

The method of Y-shaped anastomosis in complicated carcinoma of the sigmoid colon is described. It is shown that a primary colonic anastomosis can be formed by this method in intestinal obstruction.

[Combined treatment of rectal cancer using intensive macrofractional preoperative irradiation].

The article discusses the results of a cooperative randomized study according to evaluation of the efficacy of combined treatment in 451 patients among which 248 patients received an intensive course of macrofractional irradiation in cumulative local dose of--25 Gr. Intensive macrofractional irradiation increases neither the frequency of postoperative complications nor the mortality rate. At the same time, the combined method of treatment leads to a significant reduction in the frequency of a recurrent tumor and an increase in 5-year survival, particularly among patients with a stage III tumorous process.