[Bioinformatic analysis of maize granule-bound starch synthase gene].

Local alignment of Wx gene and its homolog sequences has been conducted. Phylogenetic dendrogram displaying evolutionary relationship between Poaceae members has been built basing on results of the alignment. Ancient Wx gene transfer from Zea mays to Dimeria lawsonii has been assumed.

[Allele structure of polymorphic loci of maize lines and hybrids and its correlation with heterosis level].

Polymorphism of parental lines GK26, Mo17 and lines-testers Od221MB, Od308MB, and Od329 was analyzed by SSR-PCR and genotyping of recombinant inbred lines (RIL) from populations F4, F6 was carried out. Ten microsatellite loci allele combinations of parental lines, lines-testers and the best test cross hybrids were established and their molecular-genetic formulas were derived. The microsatellites allele structure of the best for combining ability lines and high-yield hybrids was investigated.

[Association of microsatellite loci alleles of the group-5 chromosomes with frost resistance of winter wheat].

Analysis of frost resistance and microsatellite analysis of the group-5 chromosomes were performed on parental varieties and recombinant-inbred lines F7 Luzanovka odesskaya/Odesskaya krasnokolosaya. Allelic differences for Xcfd7-5B Xwmc415-5B and Xgwm 182-5D microsatellite loci were associated with the level of frost resistance of the lines.

[Clarification of Rht8 and Ppd-D1 gene linkage on the 2D chromosome of winter bread wheat].

In the south part of Ukraine the haplotype of Rht8c and Ppd-D1a genes is highly distributed among modern bread wheat varieties. During the time of breeding program it has been selected as one of the most important adaptive complex for plants of this region. Genetic distance between Rht8c and Ppd-D1a was clarified.

[Phenotypic effects of puroindoline gene alleles of bread wheat].

85 winter bread wheat varieties and lines that have been developed mostly in Ukraine were analyzed with NIR for parameters of hardness and protein content. The hardness data were compared with the data of puroindoline gene alleles analysis done earlier and the published data. Significant variation of parameters of hardness was revealed when there was low polymorphism of puroindoline genes indicating the presence of additional genes that influence the hardness parameters.

[Molecular genetic analysis of soriz genome (Sorghum oryzoidum)].

Molecular-genetic analysis of soriz genotypes (Sorghum oryzoidum), its paternal form Sorghum bicolor (L.) Moench (grain sorghum), possible parents (Sorghum sudanense (Piper.) Stapf.

[Molecular-genetic analysis of maize mitochondrion regions associated with cytoplasmic male sterility].

Molecular-genetic polymorphism of 86 world and Ukrainian breeding maize lines with S-, C- and T-types of cytoplasmic male sterility (CMS) and with normal wild type mitochondrion has been researched via mitochondrion regions PCR-analysis. Molecular marker system allowed to detect and identify definite type of CMS within maize lines, as well as to differentiate lines with definite CMS type either from lines with another CMS type or from normal wild type cytoplasm lines.

[DNA-identification of rice varieties (Oryza sativa L.) of Ukrainian selection].

Genetic diversity of 11 rice varieties of the Institute of rice UAAS was assessed using a set of 25 SSR loci. Based on analyses of 12 polymorphic microsatillite loci the procedures for composing genetic formulas of varieties and their identification have been elaborated. UPGMA-cluster-analysis based on genetic distance coefficients clearly separated all the varieties into two groups, and showed that the Ukrainian rice varieties are closely related.

[Analysis of common wheat varieties and near-isogenic lines by PCR with allele-specific primers for Gli-1 and Glu-3 loci].

The allelic characteristics of the Gli-A1, Gli-B1, Gli-D1 and Glu-A3 loci of 14 bread wheat varieties and 6 near-isogenic lines derived from Bezostaya 1 have been detected by PCR analysis. The conformity of molecular-genetic data and electrophoresis of storage proteins has been determined: the allelic variants of gliadins Gli-A1o and Gi-A1m correspond to the PCR-allele GliA1.2, the gliadin variants Gli-A1f, Gli-A1b, Gli-A1c correspond to the PCR-allele GliA 1.

[Modern biotechnologies in estimation of genetic diversity of Ukrainian varieties of hop (Humulus lupulus L.)].

Genetic variety estimation of hop gene pool using DNA-typing of highly polymorphic microsatellite loci and optimization of introduction to the culture of in vitro conditions is the important stage of national varieties resources forming, basis of modern nursery and protect mean of varieties property, and also it is necessary for development of molecular methods of selection of planting-stocks free from pathogens.

[Marking of the loci encoding maize resistance to Fusarium].

Genotyping of F2-population from crossing of maize lines Odesskaya 139 and R221 contrasting on Fusarium rot resistance has been carried out. Codominant DNA-marker RGA11 to the locus determining Fusarium resistance was found out at the distance of 18.3 cM.

[The effectiveness of selection for the DNA markers of quantitative trait loci in maize populations].

DNA-markers of some agronomically valuable quantitative trait loci were detected by SSR- and ISSR-PCR. Method of genetic improvement of initial population for polygenic trait complex is proposed basing on direct selection of plants according to their genotype marker profiles. Experimental modelling of marker selection within populations (gamma K26 x Mo17) F3-F4 has been carried out.