[Mechanisms of mitochondrial dysfunction development in vessels upon peroxidizes LDL influence].

The mechanisms of mitochondrial dysfunction development in vessels upon peroxodized LDL (pLDL) influence are discussed. Is is shown that the components of pLDL induce the formation of covalent complexes with cell proteins and destabilize cellular membrane microdomain structure that leads to membrane proteins dysfunction, Ca2+ liberation into cytosol from its stores, endoplasmic reticulum stress, increase in reactive oxygen species production by NADPH-oxidases. These effects cause disturbances in mitochondria of target cells, increases in reactive oxygen species production, mitochondrial pathway of cell death.

[Comparative analysis of mitochondrial and nuclear DNA topoisomerase I from maize].

We conducted a comparative study of the properties of topoisomerase I isolated from maize nuclei and mitochondria. We found that nuclear and mitochondrial enzymes possess different ability to bind single stranded DNA. Study of the enzyme activity dependence on Mg2+ demonstrated an absolute dependence of the mitochondrial topoisomerase activity.

[Isolation of mitochondrial DNA binding proteins which are specific for maize cox1 promoter].

We purified DNA binding proteins which interact with the promoter region of cox1 gene from maize mitochondria. Presence of poly[dIdC-dIdC] and KCl in concentrations up to 500 mM had no influence on binding efficiency demonstrating high specificity of complexes formed. Surprisingly, we did not detect DNA binding when probes containing promoter regions of other mitochondrial genes (cox3, rrn26) were used.

[Editing of the mitochondrial cox3 transcript may yield a new site of protein-protein interactions in wild cereal Elymus sibiricus L].

With PCR, RT-PCR, and direct sequencing, complete nucleotide sequences were established for the Elymus sibiricus mitochondrial cytochrome c oxidase subunit 3 gene (cox3) and its cDNA. The cox3 transcript was shown to have 12 editing sites with changes affecting the amino acid sequence of the protein product. The editing of the primary cox3 transcript was found to change the position of a site of protein-protein interactions.

[Reconstruction of the phylogenetic position of larch (Larix sukaczewii Dylis) by sequencing data for the trnK intron of chloroplast DNA].

To reconstruct the systematic relationships of larch Larix sukaczewii, we used the chloroplast trnK intron sequences of L. decidua, L. sukaczewii, L.

[Specific features of nucleic acid synthesis in mitochondria of Elymus sibiricus from different natural populations].

Conditions and kinetic characteristics of nucleic acid synthesis were studied in the isolated mitochondria of Elymus sibiricus from different natural populations. The results showed the reciprocal dependence of RNA and DNA synthesis rates in the mitochondrial genetic system of E. sibiricus seedlings of different genotypes.

Identification of highly conserved hydrophobic amino acid motif in deduced amino acid sequence of Elymus sibiricus L. mitochondrial S13 ribosomal protein.

The nucleotide sequence of mitochondrial ribosomal protein rps13 gene from wild perennial grass Elymus sibiricus is presented. It was determined by the method of PCR amplification with specific oligonucleotide primers and the direct sequencing of the amplification product. The sequence of E.

[An unusual leucine motif in amino acid sequences of subunit 9 of ATP-synthase from plant mitochondria, modified by mRNA editing].

The amino acid sequences of subunit 9 of plant mitochondrial ATP-synthase contain a leucine motif which differs from the leucine repeat earlier detected within the composition of proteinaceous products of Myc, Fos, and Jun proto-oncogens. The structural organization of this repeat in proteolipid sequences can be modified via transformation of serine triplets into leucine ones as a result of editing of an appropriate mRNA.

[Changes in oxidative phosphorylation in rat liver mitochondria during the development of cholestasis].

Kinetic patterns of oxydative phosphorylation were studied in rat liver mitochondria in dynamics of cholestasis and after addition of bile acids to mitochondria in vitro. Within the 1 and 3 days of cholestasis mitochondrial synthesis of ATP was decreased due to inhibition of ATP and ADP transport by the bile acids. At the period within 3 and 15 days uncoupling of the oxidative phosphorylation occurred; the phenomenon observed might be a result of impairment in the structure of inner mitochondrial membrane.

[Relationship between the systems of activation of fatty acids and ademine nucleotide translocase in the mitochondria].

Experiments were conducted on freshly isolated rat liver mitochondria and mitochondria subjected to ageing by two different methods. It was shown that the work of the mitochondrial system of fatty acid activation could lead to inhibition of the adenine nucleotide transport through the internal mitochondrial membrane. Inhibition of adenine nucleotide translocase was eliminated by preincubation of mitochondria with carnitine.

[Mitochondrial respiration and the state of adenine nucleotide phosphorylation in rat liver following multiple administration of 3-methylcholanthrene and phenobarbital].

After 4 injections of phenobarbital (PB), 3-methylcholantrene in olive oil (MC) or olive oil alone the corresponding concentrations of acyl-CoAs in the liver (in per cent to control) were: 73, 167, 230. The liver mitochondria from rats injected with oil and MC showed inhibition of the metabolic state 3 respiratory rate after Chance, Preincubation with carnitine elimated this inhibition. In accordance with these data the blood ketone body level in the administration of PB, MC and oil were 31, 136, and 342%, respectively.

[Possible role of adenine nucleotide transport in regulating the respiration of rat liver mitochondria].

Studies with liver mitochondria from rats which starved for 48 hours showed the rate of ADP-stimulated respiration to be 20% lower than in the presence of an uncoupler. This effect was eliminated by preincubation of mitochondria with carnitine. Mitochondria from fed rats were characterized by a considerable decrease of states 3 and 4 respiration.