The effect of the medium pH on accumulation of [3H]-cauloside C by tumor cells, its intracellular localization, and interaction of the glicoside with membranes of tumor cells and liposomes has been studied. The shift towards weakly acids pH leads to the increase in the amount of cauloside C accumulated by tumor cells and changes the pattern of interaction of cauloside C with the membranes.
View Article and Find Full Text PDFAn ATP-dependent DNAse was isolated from the cells of the sea urchin Strongylocentrotus intermedius embryos by chromatography on DEAE-cellulose and gel chromatography on Sepharose 4B. The enzyme was found homogeneous during polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined by gel filtration through Sepharose 6B and was equal to approximately 450,000.
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