[Computerized analysis of inverted repeats in Mycobacterium tuberculosis genome].

Thermodynamic stable inverted repeats, capable of stabilizing nuclease-influenced mRNA, have been determined for M. tuberculosis slowly growing isolates H37Rv and CDC1551 with the completely sequenced genome. The genome of laboratory strain H37Rv may contain 50 pin structures formed by inverted repeats, whose stem varies in length from 11 to 28 nucleotide pairs (n.

[The role of immunological mechanisms in the development of the late sequelae of nuclear disasters].

Effects, both specific and non-specific, were analysed of ionizing radiation in a comparative evaluation of medical sequels of the Chernobyl disaster and of the Semipalatinsk Range nuclear test. A hypothesis is advanced assuming that development of all major bodily morbid conditions is directly related to the dysfunction of certain complex of organs, the thyroid gland in the first place, the thymus gland known to be a central organ of systemic immunogenesis. A breach in the thymus link triggers harmful cascade reactions of neuroimmunoendocrinological regulation, appeared to be responsible for the pathogenesis of practically all pathologies disclosed.

[The possible role of immunogenetic factors in the pathogenesis of HIV infection].

In this review the data on the presence of associations between immunogenetic markers and the development of HIV infection are presented. Special attention is given to spatial relationships of genetic determinants, responsible for the synthesis of the components of the complement system on one hand and the genes controlling resistance to the causative agents of opportunistic infections and malignant growth on the other hand. Suggestion is made on close relationship between the specific features of the course of HIV infection and the genetic control, responsible for the synthesis of complement components, in particular the genes making up the HLA system.

[The use of the fluorescent probe method for the diagnosis of acute coronavirus intestinal infections in people].

The scientific investigations conducted first allow using a method of fluorescent probes for the diagnosis of human coronaviral intestinal infection. The method is characterised by sensitivity, specificity, reproductivity and proximity. It is comparable, according to these parameters, with the methods of the third generation and may be recommended for putting into practical work of virological laboratories.

[The clinical picture of internal diseases in the light of immunology].

Clinical profiles of internal diseases are examined from the immunological standpoint together with the causes for creation by immunology of its own clinical base. It is shown that the approach common in immunology, based on the fundamental notions of the immunity system offers superior efficacy and should be recommended in dealing with internal diseases in a clinical setting.

[The antimicrobial activity of a new wound-healing compound ointment].

Drug composition containing polyethyleneoxide PEO-400 and PEO-1500 (8:2), antimicrobe agent-chloramphenical as well as new antiinflammatory mean of plant origin EOL in concentration of 5% has been created. Introduction of EOL to the composition of the drug under elaboration permitted decreasing chloramphenicol concentration 2-4 times as compared to concentrations used in contemporary ointments. This has lowered toxic and allergenic properties of the drug suggested for healing the infected wounds.

[The characteristics of the biological properties of Listeria strains isolated in Ukraine and Belgium].

Biological characteristics of 21 strains of bacteria of Listeria genus have been studied in Ukraine and Belgium. Morphological, cultural biochemical properties of cultures have been described. It has been proved that 85.

[Microbiological studies of stomatological periodontal films containing a derivative of chlorocinnamic aldehyde].

Stomatological parodontal films removing the effect of negative factors on the parodontal have been studied. Antibacterial effect of the derivative of chlorocinnamic aldehyde, composition ingredients and their different combinations as well as the films as a whole have been studied when creating stomatological parodontal films.

[Optimizing conditions of cell culture use for virus indications by means of fluorescent probes].

Cell cultures in a suspension on medium 199 with 1 x 10(6) cells per ml are recommended for virus indication by means of fluorescent probe method. When stored at 4 degrees C, the cells are fit for work for 5 days. The proposed conditions for the use of cell cultures are necessary for effective introduction of the fluorescent probe method in practical virological laboratories.

[A model of the structure of the receptor apparatus of HIV target cells (1)].

A model is proposed and well based of structure of the receptor apparatus of interacting HIV target cells: Langerhans cells (LC) and helper cells (HC)--designed to explain feasibility of realizing a double activation signal, together with some features of immune pathogenesis of HIV-infection (morphofunctional abnormalities in certain subpopulations of the immune system such as HC, suppressor cells, killer cells, natural killers, lymph cells, macrophages, LC), as well as immunogenetic predisposition to AIDS.

[The structure and immunobiological properties of coronavirus proteins].

The survey includes data concerning structural proteins of coronaviruses obtained for the last ten years. It elucidates such problems as the proteins organization in the virion, heterogeneity of their composition, structure and biological properties, antigenic and immunobiological properties, prospects of creation of specific immunogenic anticoronavirus, prophylactic and diagnostic preparations.

[The effect of dexamethoxin on the integrity of cytoplasmic membrane in gram-positive and gram-negative microorganisms].

Decamethoxin is shown to be able to increase membrane permeability of Pseudomonas aeruginosa, Escherichia coli and Micrococcus lysodeikticus, that is confirmed by a loss of compounds with the absorption maximum at 260 nm by cells. Parallel with this the number of viable individuals has fallen and activity of dehydrogenases has been inhibited. The aspartate and alanine aminotransferase activity was not inhibited by decamethoxin and even increased.

[Identification of bis-quaternary ammonium compounds using mild ionization mass spectrometry].

Ethonium, an antimicrobial chemotherapeutic agent, was investigated by mass spectrometry (MS) under various ionization conditions: electron impact, field ionization, field desorption (FD) and fast atom ionization. FDMS was found to be the most suitable procedure for ethonium identification. Relation of the ED mass spectra to the distance between the nitrogen atoms in bis-quaternary ammonium compounds is discussed.

[The study of complex-formation of DNA with the antimicrobial drug decamethoxine].

The interaction of effective antibacterial drug decametoxyn with natural DNA was studied by UV-spectroscopy. Decametoxyn shows a specificity to nucleotides: it decreases the cooperativity of melting and the thermal stability of DNA parts enriched by AT pairs. The characteristics of the helix-coil transition on the DNA parts enriched by GC-pairs are invariable.

[Mass-spectrometric analysis of an anti-microbial preparation decamethoxine].

I. I. Mechnikov Kharkov Research Institute of Microbiology, Vaccines and Sera, Ministry of Public Health of the Ukrainian SSR.

[Antimicrobial activity of chlorine-substituted 4-alkyl- and arylphenols].

The results of the study on the antimicrobial activity of 12 chlorine-substituted 4-alkyl- and arylphenols against Staphylococcus, Streptococcus, E. coli, Salmonella typhosa, Salmonella typhimurium, Shigella dysenteriae, Proteus, Klebsiella rhinoscleromatis, Candida, Trichophyton gypseum and Microsporum lanosum are presented. It was found that introduction of the alkyl substitute to position 4 of orthochlorphenol increased the activity of the substances against the majority of test microbes.

[Effect of gentamycin, decamethoxin and prodigiozan on the course and outcome of experimental pyocyanic infection in white mice].

The therapeutic and prophylactic effectiveness of gentamicin, decamethoxin and prodigiozan was determined on albino mice infected with two-fold lethal doses of the antibiotic-resistant strain of Ps. aeruginosa. Correlation between the effectiveness of the drug and the dose and time of its administration was found.

[Microbiological characteristics and the sensitivity to antibiotics of the Pseudomonas pyocyanea circulating on breeding stations].

Biological properties and sensitivity to antibiotics and decametoxin of 464 strains of Ps. aeruginosa isolated at Pedigree Cattle Stations in the Chernovitsy region were studied in vitro. The isolates proved to be resistant to penicillin.