[Results of parasitological passportization of sewage treatment works in the South Federal District].

Developed parasitological passports for sewage treatment works have been issued in the subjects of the Southern Federal District. Its some regions have areas inadequately covered by centralized water drainage. New waste treatment technologies and disinvasion agents have not been virtually introduced.

[Biological characteristics of the causative agent of dirofilariasis in dogs in the Rostov Region].

This paper assesses the trends in the identification of different Dirofilaria species in the Rostov Region and comparatively analyzes the general clinical and biochemical blood indices in dogs with and Without helminthism. Seasonal variations in the invasion and species composition of the parasite have been established. At present, there is a gradual replacement of the species Dirofilaria repens by Dirofilaria immitis in this area.

[The sanitary and parasitological characteristics of environmental objects in the south of Russia. Wastewaters and their precipitations].

The relative uniformity of helminthic eggs has been noted in the wastewaters of the Rostov Region, Krasnodar Territory, and the Republic ofAdygea. An ovogram is mainly presented by the eggs of Ascaris, Toxocaras, and whipworms. By taking into account incomplete dehelmintization during waste treatment, 5,000 to 1,080,000 helminth eggs may annually come into the water reservoirs where purified wastewaters are discharged, 33% remaining viable.

[The epidemiological and clinicoimmunological aspects of the outburst of trichinosis in the Krymsky District of the Krasnodar Territory].

The present paper presents the epidemiological and clinicoimmunological aspects of the outburst of trichinosis in the Krymsky District of the Krasnodar Territory in January 2001. Six hundred and forty-eight persons ate infested wild boar meat, 119 (74.8%) fell ill with moderate and severe trichinosis.

[Impact of effect of natural disasters on the circulation of causative agents of parasitic diseases].

The southern region is marked by a high incidence of parasitic diseases and a significant contamination of environmental objects with the eggs and cysts of their pathogens. Background examinations revealed the greatest soil contamination with helminthic eggs in the Temryuksky District of the Krasnodar Territory and in the towns of Vladikavkaz and Digora of the Republic of North Ossetia (Alania). The least contamination was found in Rostov-on-Don and the towns of the south-western area of the Krasnodar Territory.

[Preparation of a dry red blood cell Trichinella antigenic diagnosticum for indirect hemagglutination test and evaluation of its efficiency].

The present paper presents the stages of a process for manufacturing a dry red blood cell Trichinella antigenic diagnosticum for indirect hemagglutination test (IHAT) and the evaluation of its diagnostic efficiency. The diagnosticum is shown to be a 3% suspension lyophilized from formalinized and tanned sheep red blood cells on which an excretory-secretory antigen of invasion Trichinella larvae was absorbed in a dose of 100 mg/ml. The rather high sensitivity (98.

[Trichinosis in the Republic of North Ossetia-Alania].

The present paper presents the biological, epidemiological, clinical, and immunological characteristics of trichinosis in the Republic of North Ossetia-Alania, which is based on the results of long-term studies and follow-ups and on the 1971-2002 report data from medical and veterinary services and hunt inspectorate agencies. It has established that a total of 675 clinical cases of trichinosis were recorded in Ossetia-Alania in the period under study. The incidence rate of the disease ranged from 0.

[The detection of pathogenicity factors in the nematode Trichinella spiralis at different stages of its development].

The study of water soluble proteins of Trichinella spiralis has discovered for the first time some protein fractions having neiraminedase, haemagglutinative and cytotoxic activities. The presence of hyaluronidase was confirmed and its activity both for water soluble and cuticle proteins of the trichinella was examined. The haemagglutinin and cytotoxin of the trichinella have an antigen specificity.

[A comparative electrophoretic analysis of the isoenzymes in "reference" and natural strains of Trichinella].

In four enzymes among eight ones examined the differences were revealed in the intensity of staining of certain zones (G-6-P, ES) as well as in electrophoretic mobility of some loci (PGD, RGM) between the capsule forms of trichinellae and Trichinella pseudospiralis. As a result, the validity of isolation of T. pseudospiralis Garkavi, 1972 is confirmed.

[Soluble metabolic antigens of Trichinella spiralis: their isolation and characteristics].

Cultivation of Trichinella muscular larvae, purified by centrifugation in 20 ...

[The resistance of muscle larvae of Trichinella spiralis to exposure to low temperatures].

It has been shown that the resistance of muscular larvae of Trichinella spiralis to the effect of low temperatures depends on the conditions of freezing and composition of media possessing cryoprotective properties. It has been established that under optimal conditions (medium of microenvironment, conditions of freezing) during the storage in liquid nitrogen muscular larvae of T. spiralis preserve 50 to 60% of their resistance by the character of mobility and 11 to 15% by their capacity for reproduction.

[The reproductive activity of Trichinella spiralis in the early stages of cultivation on artificial nutrient media].

Reproductive activity of mature Trichinella spiralis was studied during its cultivation in three nutrient media. Most active hatching of Trichinella larvae was observed in Erl's saline, less active in Eagle's medium and in the lactalbumin hydrolyzate medium. In Erl's medium the hatching of larvae accounted for 80% of their total mass for the first four hours of the experiment and the absolute number of larvae was 2.

[Intrastrain heterogeneity of Trichinella spiralis: a sedimentation analysis].

Intrastrain heterogeneity of muscular larvae of trichinellids has been revealed in experiments of free sedimentation and isopyknic division in the density gradient of saccharose, the presence of which is confirmed by the study of morphological characters and infection activity. The presence of intrastrain heterogeneity indicates the necessity of introduction of standard technique for studies of biological characters of different strains of trichinellids.

[Development of a solid-phase immunoenzyme method for determining the antibody level in the blood sera of persons vaccinated with influenza vaccine].

To control the effectiveness of vaccination against influenza, the optimum conditions for making the enzyme-linked immunosorbent assay (ELISA) with a view to determine the level of anti-influenza antibodies in human blood sera have been established. The kinetics of influenza virus adsorption in the wells of ELISA polystyrene plates and the kinetics of the interaction between the immobilized antigen and species-specific peroxidase-labeled antibodies have been studied. The method has been shown to be more sensitive than the hemagglutination inhibition test in the determination of seroconversion in persons immunized with influenza vaccine.

[Current problems of the epidemiological surveillance of poliomyelitis].

A uniform program of the epidemiological supervision of poliomyelitis must be worked out for every administrative territorial unit. This program should include the early and complete detection of all cases of this infection, irrespective of their severity; the analysis of the quality of vaccination; the determination of the immune status of the child population; the characteristic of the poliovirus circulation.

[Erythrocyte test preparations for standardizing fluorescent antibodies and the serological diagnosis of influenza].

An erythrocytic diagnostic preparation, obtained by the sandwich method and intended for the standardization of antivirus fluorescent antibodies (FA) and the determination of specific IgM in the blood sera of patients with a view to the early serological diagnosis of influenza, has been proposed. The loading of immunosorbents with influenza virus, achieved in this preparation, approaches its theoretical limit, the virions being arranged in a single layer, and constitutes up to 0.4 hemagglutinating units per 1000 red blood cells.

[Use of an immunofluorescence micromethod for determining the antigens of the influenza and tick-borne encephalitis viruses].

The diagnostic possibilities of several variants of the solid-phase immunofluorescent micromethod (requiring 100-200 microliter of the reaction mixture), intended for the determination of influenza virus and tick-borne encephalitis virus antigens in material obtained from patients and ticks, have been studied. A high degree of correlation between the results obtained by the methods under investigation and the control methods has been established.

[Immunoenzyme analysis. Quantitative determination of antibodies to the influenza virus].

Solid-phase immunoenzymatic procedure for quantitative estimation of antibodies to virus of influenza A was developed. A relationship was found between the rate of influenza virus adsorption on polystyrene and concentration of the cells, duration of the adsorption, temperature and the nature of buffer systems. Optimal conditions were developed for binding of the virus with polystyrene, immune sera and the conjugate IgG-peroxidase.

[Circulation of influenza A virus (H3N2) and its role in the occurrence of epidemics 1976-1981].

In 1976-1981 the etiology of influenza epidemics was mixed, influenza viruses A(H3N2), A(H1N1) and B taking part in them simultaneously. The active circulation of influenza virus strains with different antigenic structures was established: influenza virus A(H3N2) circulated in 1976, and since November 1977 simultaneous spread of influenza viruses A(H3N2) and A(H1N1) was observed, the epidemic activity of strain A(H3N2) being noticeably decreased during this period; in the autumn and winter months of 1979-1980 this strain appeared as the dominating infective agent. After intensive epidemics during the summer months of 1976 and 1980 influenza virus strains A(H3N2), antigenically related to the causative agent of the previous epidemic, were isolated from patients with acute respiratory infection.

[Characteristics of the dynamics of herd immunity to the poliovirus and of the quality of the vaccination carried out against poliomyelitis].

The aim of this work was to study the state of immunity in children immunized with liquid poliomyelitis vaccine. During 1976-1981 the intensity of immunity to poliomyelitis in 653 children aged 1-3 years was studied. The study showed that at the period when liquid poliomyelitis vaccine was used the share immune to polioviruses, types 1 and 2, constituted 89.