[The isolation of the surface antigen from vegetative cells of Bacillus anthracis STI-1 and study of its protective properties].

The method for the extraction of native surface protein antigen with a mol. wt. of 92 kD from vegetative cells of B.

[Experimental anthrax infection in laboratory animals with differing species susceptibility to the causative agent].

In guinea pigs and noninbred white mice, infected subcutaneously with anthrax which resulted in their death, characteristic generalized infection with the hematogenic contamination of their organs and the signs of intoxication and shock could be observed. In inbred white rats (Fisher 344) the invasion and dissemination of B. anthracis are relatively slightly pronounced, the phenomena of intoxication and shock being clearly prevalent.

[The use of plasmid screening for the differentiation of Bacillus anthracis strains from closely related species of soil bacilli].

B. anthracis virulent and vaccine strains differ from the strains of species closely related to B. anthracis, such as B.

[Specific intoxication in anthrax infection].

The pathomorphological picture of experimental B. anthracis infection in white rats (strain Fisher-344) essentially corresponds to experimental anthracic intoxication with very moderately pronounced morphological manifestation of B. anthracis invasion.

[In vitro amino acid requirements of Bacillus anthracis cells].

In the process of batch cultivation in a synthetic medium B. anthracis cells actively consume free amino acids. The maximum amino acid consumption per unit of biomass occurs at the exponential phase of growth, but the consumption of serine is maintained at a high level also during the stationary phase.

[Nutrient requirements of Bacillus anthracis strains and the growth characteristics of strain STI-1 in batch cultivation on synthetic broths].

A synthetic culture medium is proposed. This medium satisfies the nutritional requirements of most cultivated strains under study by a single amino acid: valine. The possibility of detecting strains with other nutritional requirements has been shown.