[Purification and ligand-receptor analysis of insulin-related peptides from pedal ganglion of the mollusc Anodonta cygnea].

Six insulin-related peptides (IRPs) from pedal ganglions of the molluscs Anodonta cygnea have been isolated and purified by reverse-phase chromatography. Each peptide (designated as IRP8-IRP13) showed its own retention time on the HPLC column. The testing of IRPs in radioreceptor systems specific for insulin and insulin growth factor-I (IGF-I) showed their ability to bind to both types of receptors.

[The growth-stimulating activity of the insulins from teleosts].

Growth-stimulating properties of insulins from cyclostomes, teleosts and mammals were evaluated by their capacity to promote the uptake of [35S]-sulfate by fish branchial cartilages. It was shown that all the tested hormones exhibit promotor properties increasing the uptake of labeled sulfate by 1.5-3 times.

[The mitogenic effect of insulin isolated from the mammalian brain on Swiss 3T3 cells].

Mitogenic properties of the insulin derived from pig brain were compared with the action of pancreatic (standard) pig insulin and epidermal growth factor (EGF) using the culture of Swiss 3T3 cells. The brain insulin, likely as the pancreatic insulin, induced uptake of 14C-thymidine by resting cells in a dose-dependent manner at concentration of 0.5-2.

[Amino acid sequence of the insulin of the dog salmon Oncorhynchus keta].

It has been demonstrated that A-chain of insulin in the salmon O. keta consists of 21 amino acid residues with N-terminal glycine and C-terminal asparagine. B-chain is formed by 29 residues with alanine and lysine as N- and C-terminals correspondingly.

[Comparative study of the immunological properties of insulins and insulin-like substances of vertebrates and invertebrates].

Comparative studies have been made on immunological properties of insulins (bovine, porcine and fish--Oncorhynchus gorbuscha, O. keta) and insulin-like substances of the mollusk Anodonta cygnea and starfish Asterias rubens in homologous and heterologous for these hormones radio-immune systems. It was concluded that each of these systems reveals in the insulins and insulin-like substances investigated the identical antigenic determinants.

[Preparation of an insulin-like substance from molluscs and its antiserum].

A substance with insulin-like activity was obtained from visceral organs (excluding foot, gills and mantle) of the freshwater bivalve molluscs Unio pictorum and Anodonta cygnea. Injecting this substance to guinea pigs, antiserum was also obtained. Using the obtained antiserum in immunostaining experiments, the cells which produce insulin-like substance were identified in the intestinal epithelium of the midgut in A.

[The isolation and purification of insulins from the pink salmon Oncorhynchus gorbuscha and the walleye pollock Theragra chalcogramma].

Crystalline insulins have been obtained from the insular tissue of the teleosts O. gorbuscha and Th. chalcogramma which is located separately from the exocrine tissue.

[Corticosteroid concentration in the blood of the Russian sturgeon, Acipenser güldenstädti, at different stages in its life cycle].

Corticosteroid content of the blood has been measured during upstream and downstream migrations as well as during sea period of life of the sturgeon A. galdenstädti. It was shown that during anadromous migration before spawing, the level of corticosteroids in the blood increases, the increases beginning already during the sea period.