[Isolation and cultivation of a swine rotavirus].

A swine rotavirus capable of inducing the cytopathic effect was isolated in a roller culture of Macaca rhesus kidney cells (line MA-104) after two preliminary passages in gnotobiotic piglets and colostrum-free piglets, and the isolate was designated strain K. For virus isolation, fecal specimens were treated with trypsin, and besides, trypsin was added into the maintenance medium. After 20 passages in MA-104 cell culture the swine rotavirus was adapted to pig embryo kidney cell cultures (SPEV line) in which the maximum virus accumulation, 8.

[Method of preparation of the specimens for combined immunocytological examinations by light and electron microscopy].

A method for making preparations for combined immunocytological examinations in light and electron microscopes is described. An infected cell culture grown on slides (a piglet kidney cell culture infected with Teschen virus) was fixed with 2.5% glutaraldehyde in phosphate buffer, pH 7.