[Study of the phenotypic occurrence of ura gene inactivation in Bacillus subtilis].

After inactivation of the ypaA gene in Bacillus subtilis, the phenotypic pattern obtained showed that this gene controls a system for active flavin transport and, possibly, riboflavin excretion under the conditions of constitutive synthesis.

[A conservative compression surgical orthodontic method for treating upper prognathism in adults].

A clinical example of effective treatment of an adult patient with combined deformations of the jaws (upper prognathism of the third degree and open occlusion of the second degree) is reported. Fragmented osteotomy of the maxilla at the level of symmetrical removal of second molars is performed. Due to Engle's arches soldered with Vasil'ev's splint and compression, a maxillary fragment was shifted 10 mm backward and downward at the expense of compressing the inter-root septae; the first molars were preserved.

[Cloning and biochemical identification of the ribR gene in Bacillus subtilis].

The PCR copy of the ribR gene of Bacillus subtilis was subcloned in Escherichia coli cells under the control of the phage T7 inducible promoter. The polypeptide of 26 kDa corresponding to the 690-bp gene is the product of the ribR gene. The protein encoded by the ribR gene is flavokinase, and the riboflavin-reduced form is the substrate for it.

[Experience in the treatment of mandibular condyle fractures at specialized military medical institutions].

The authors have generalized the experience of treatment of 82 injured with injuries of condylar processus fracture treatment of the lower jaw. By a surgical way were successfully eliminated posttraumatic ankylosis and contracture of temporomandibular articulations with use of titanium endoprosthesis and semi-joints. The osteosynthesis of condylar processus of the lower jaw by titanium miniplates and screws was also practiced.

[Analysis of the primary structure of the supplementary regulatory region of the riboflavin operon in Bacillus subtilis].

The sequencing of a 3.5 kb EcoRI-BamHI fragment located in the 236 degrees region of the Bacillus subtilis chromosome revealed a 690-bp long open reading frame, partly similar in amino acid composition to flavokinases/FAD synthases from several microorganisms. The discovered sequence can be identified with the gene ribR, an auxiliary regulatory gene of the riboflavin operon of Bacillus subtilis.

[Cloning of ribR, an additional regulatory gene of the Bacillus subtilis riboflavin operon].

A 13.0-kb EcoRI fragment of Bacillus subtilis DNA carrying an additional regulatory ribR gene of riboflavin operon was cloned on the basis of resistance to 7, 8-dimethyl-10 (O-methylacetoxym)-isoalloxasin. The cloned fragment was trans-dominant with regard to ribC constitutive mutations that block the overproduction of riboflavin but inactive relative to constitutive mutations in the rib(O) regulatory region.

[Phenotypic expression of a 100-pair nucleotide deletion in the regulatory region of the Bacillus subtilis riboflavin operon].

Assessment of specific activity of riboflavin synthase and the level of riboflavin accumulation in strains with a 110-nucleotide deletion in the regulatory region of the riboflavin operon showed that this deletion specified semi-constitutive expression of the operon. This was assumed to be connected with the elimination of three nucleotides from a potential transcription antiterminator.

[The catalase activity and lipid peroxidation of donor blood under ultraviolet irradiation].

Under study were catalase activity and indicators of lipid peroxidation (LP) of donor blood exposed to different doses of UV irradiation. Doses lower than 630 J/m2 were found to activate catalase and to inhibit LP while doses higher than 630 J/m2 inhibited catalase activity and activated LP. The indicators of LP have confirmed that therapeutic doses of UV-irradiated blood were nontoxic.

[Isolation and analysis of protease-deficient mutants of Bacillus amyloliquefaciens].

Four types of protease negative mutants of Bacillus amyloliquefaciens A50 were selected after four stages of step-by-step UV light mutagenesis. EDTA, and PMSF were used as inhibitors of protease activity to characterize the protease negative mutants with regard to the protease type. The electrophoretic patterns of proteases from culture medium of B.

[Elimination of introns from the interleukin 1 beta genome by DNA amplification and expression of interleukin 1 beta in Escherichia coli].

The use of the polymerase chain reaction was proposed for intron excision from genomic genes with known nucleotide sequences. Three exons (5, 6 and 7) of genomic interleukin 1 beta gene were amplified by means of thermostable DNA polymerase TthI from Thermus thermophilus on the base of cloned in M13 phage human genomic interleukin 1 beta gene. Synthetic oligonucleotides complementary to sequences flanking exons were used as primers.

[Streptavidin secretion by Streptomyces lividans strains].

Recombinant strains of S. lividans capable of secreting streptavidin were isolated. Various constructions containing either streptavidin gene copies integrated within a chromosome or a streptavidin gene within the secretory vector were investigated.

[Mutations in the alpha-amylase gene of Bacillus amyloliquefaciens, leading to a decrease in the temperature of protein inactivation].

Alpha-amylase genes of Bacillus amyloliquefaciens, coding proteins with reduced thermostability, had been obtained as a result of hydroxylamine mutagenesis. Temperature, pH and starch concentration dependences of two mutant alpha-amylases were investigated. The synthesis of the alpha-amylases by several B.

[Two-codon mutagenesis of alpha-amylase gene of Bacillus amyloliquefaciens].

The oligonucleotide encoding Bam HI recognition site having the structure pCGGGATC had been inserted into the recognition sites MspI of the B. amyloliquefaciens alpha-amylase gene, which was cloned in pTG29B plasmid. The alpha-amylase gene had no BamHI sites before mutagenesis.

[Conjugation transfer of the plasmid pAMbeta1 into various Bacillus species].

Streptococcal broad host range plasmid pAM beta 1 was transferred by a conjugation-like process from Streptococcus faecalis to 13 strains of different Bacilli species. In intraspecies matings the frequencies of transfer of pAM beta 1 varied from 2.10(-5) to 1.

[Mutations in Escherichia coli pmp and htpR genes stabilize the products of foreign gene expression].

The half lives of mRNA for Escherichia coli chloramphenicol-acetyltransferase, Bacillus amyloliquefaciens alpha-amylase and human leucocyte interferon were measured in E. coli cells by molecular RNA.DNA hybridization.

[Replication of Streptomyces plasmids: the DNA nucleotide sequence of plasmid pSB 24.2].

The nucleotide sequence of DNA in plasmid pSB 24.2, a natural deletion derivative of plasmid pSB 24.1 isolated from S.

[Isolation and study of hybrid plasmids carrying Escherichia coli threonine operon genes].

A fragment of Escherichia coli chromosome containing the intact threonine operon or its distinct genes has been cloned on the pBR322 plasmid. This fragment has been mapped using some restriction endonucleases. Cloning results in an increased level of appropriate enzyme activity in cells containing hybrid plasmids.

[Instability study of Escherichia coli strains containing hybrid plasmids with threonine operon fragments].

The stability of Escherichia coli strains carrying hybrid plasmids which contain ColE1-like replicon and threonine operon genes was studied. It was shown that the main reason for instability is the loss of a plasmid. The second reason for instability is the rec-dependent recombination that leads to formation of new plasmids.

[Kinetics of the reactions joining DNA fragments, catalyzed by DNA-ligase. II. Heterogeneous mixture of fragments. Optimization of the reaction conditions].

The system of kinetic equations was proposed describing the joining of heterogeneous DNA fragments by DNA-ligase. The DNA fragments were considered as flexible rods and characterized only by molecular weights and concentrations. The program for calculating the concentrations of all circular and linear DNA forms is described.

[Interaction of RNA polymerase with hybrid plasmids carrying Escherichia coli threonine operon].

The RNA polymerase binding with two hybrid plasmids carrying threonine operon genes was studied. RNA polymerase binding sites were localized using nitrocellulose binding assay and electron microscopy visualization of the RNA polymerase--DNA complexes. To confirm that observed RNA polymerase binding sites are real promoters we analyzed RNA transcripts synthesized in vitro by hybridization with DNA fragments.