[Mechanism of the toxic action of copper nanoparticles on Escherichia coli bacteria].

Some features of contact interaction of Escherichia coli with Cu nanoparticles 75-100 nm in diameter have been studied. The analysis of variations in the amplitude of fluorescence of the negatively charged probe 1-anilinonaphthalene-8-sulfonate (ANS) in Gouy-Chapmen coordinates indicates an increase (in the absolute value) in the electrostatic potential of E. coli external membrane during the contact with Cu nanoparticles.

[Structural modifications of the surface of Escherichia coli bacteria and copper-induced permeability of plasma membrane].

The effect of Cu2+ on the structural organization of the cell surface of Escherichia coli bacteria during the induction of conductivity of a plasma membrane was studied. A fluorescent study did not reveal any substantial changes in the microviscosity of lipids by the action of copper ions. At the same time, a substantial reorganization of membrane proteins during plasmolysis was observed.

[State of the hemostasis system in Poland's syndrome].

The results of a complex investigation of the system of hemostasis in 14 women with Poland's syndrome have shown combined thrombocytic-coagulational disorders, symptoms of hypocoagulation. The functional properties were impaired against the background of the normal or moderately reduced number of thrombocytes. The data of examination, case history and results of investigation of the hemostasis system show the Poland syndrome to be a variant of mesenchymal dysplasias.

[The role of reactive oxygen species in copper-induced permeability of plasma membranes in Escherichia coli].

The role of active oxygen species in the induction of nonselective cationic permeability of the plasma membrane of bacteria Escherichia coli B by the action of Cu2+ ions was studied. It was found that the increase in the amount of active oxygen species in the suspension after treating cells with copper occurred synchronously with the leakage of K+ cations from them. Evidence is presented that active oxygen species formed during the interaction of copper ions with bacteria under aerobic conditions are not involved in the induction of channel conductivity in the membrane.

[Reducing centers on the surface of Escherichia coli bacteria and their role in copper-induced plasma membrane permeability].

The reducing properties of Escherichia coli and their role in the induction of nonselective cationic permeability of plasma membrane by the action of Cu2+ ions were studied. The ability of cells to reduce exogenous dithiopyridine was shown to be maximal in freshly collected culture and to decrease upon starvation or exhaustion of bacteria by dinitrophenol, in the presence of other oxidants of cell thiols in the medium, and after the disturbance of the barrier properties of membrane by tetrachloracetic acid or butanol. The alkylation of cell thiols accessible for N-ethyl maleimide completely disrupted the reducing activity of bacteria.

[Cu2+-induced permeability of the Escherichia coli cytoplasmic membrane].

Cu(2+)-induced permeability of cytoplasmic membranes of Escherichia coli for different cations and neutral molecules of saccharose was estimated by studying their effect on cell plasmolysis during uncharged exchange of cytoplasmic K+ ions by periplasmic space cations. The addition of copper resulted in the exchange of K+ ions by periplasmic Na+, Tris+, streptomycin2+, Cu2+, Ca2+, Mg2+, Cd2+, and Mn2+. It is concluded that Cu(2+)-induced conducting pathways in bacterial membranes are hydrophilic channels with a radius of approximately 0.

[Liposuction].

Hemostasis was studied in 38 women aged from 24 to 40 years before the liposuction procedure and 2-3 days after it. The results obtained evidence for substantial postoperative hypercoagulation changes with simultaneous inhibition of antithrombine and fibrinolytic activities. Signs of thrombophilic inclination as a result of hematocrite lowering due to hypervolemic hemodilution.

[Analysis of the binding site of copper with intact and Escherichia coli bacteria modified by N-ethylmaleimide by ESR].

Characteristics of copper binding sites in the bacteria E. coli were studied using ESR spectroscopy. It was found that these cations had high local density on the strong binding range represented by the two type sites.

[The role of oxygen in the inhibition of Escherichia coli respiration by copper ions].

Effect of Cu2+ on E. coli respiration and the role of oxygen in toxic action of copper has been studied. Stimulation of respiration is observed at initial time after introduction of Cu2+.

[The copper-dependent accumulation of magnesium in Escherichia coli].

Cu2(+)-induced accumulation of Mg ions by E. coli cells has been studied. The accumulation was demonstrated to take place only when the cell had endogenous energetic resources.

[Heavy metal cation-induced increase in the antimicrobial activity of gramicidin S. Increased sensitivity of metal-resistant mutants of Escherichia coli B to the antibiotic].

Gramicidin S response of metal resistant mutants of E. coli B and the effect of concentrations of Cu2+, Ag+, Co2+ and Cd2+ on the growth and sensitivity of E. coli B to cationic antibiotics, i.

[The effect of CuCl2 on the efflux of inorganic phosphate from Escherichia coli].

The influence of CuCl2 on inorganic phosphate efflux from resting E. coli and those treated with glucose has been studied. Maintaining of high phosphate gradient on the membrane is possibly only in case of continuous supply of external metabolic energy.

[The trace element composition of the organs and tissues of animals administered a highly dispersed powder and copper sulfate].

Changes in the content of microelements in blood, heart and liver after a single subcutaneous injection of aquatic suspension of highly dispersed powder and copper sulphate in different doses were investigated. Injection of highly dispersed powder and copper sulphate was shown increase phase fluctuations of copper, zinc and iron level in all organs under study. It was demonstrated that irrespective of the form and dose of injection copper does not break interrelation modus between zinc and copper, characteristic of the control and does not change zinc/copper ratio.

[Changes in the level of sulfhydryl groups in the organs and blood of mice administered a highly dispersed iron powder against a background of developing anemia].

Alimentary iron deficiency causes significant changes in thiol content: the increase in the content of protein sulfhydryl groups in organs at late stages and the decrease in that of non-protein sulfhydryl groups at early stages of experiment. A preliminary introduction of 10 mg/kg fine iron powder (FIP) into animals fed iron-free food leads to the decrease in sulfhydryl group content in organs and blood of experimental mice. The decrease in sulfhydryl group content at FIP introduction might be connected with changes in activity of glutathione-dependent enzymes.

[The role of surface and transmembrane potentials in copper binding by Escherichia coli cells].

Cu2+-induced accumulation of Mg2+ ions by E. coli cells has been studied. The accumulation was demonstrated to take place only when the cell had endogenous energy resources.

[Effect of a highly dispersed copper powder on superoxide dismutase and glutathione peroxidase activities in experimental myocardial infarct].

Administration of highly dispersed copper powder in a dose 0.2 mg/kg three days before modelled coronary-occlusion myocardial infarction caused transitory increase in activity of antioxidant enzymes--superoxide dismutase and glutathione peroxidase in the necrotic zone of myocardium of rats, and also staunch increase in the activity of these enzymes in noninfarction region.

[The effect of surface and membrane potential on 1-anilino-8-naphthalenesulfonate binding with E. coli membrane].

Dependence of ANS fluorescence on the surface potential of E. coli under lowered resistance of the bacterial membrane and after application of the positive diffusion potential inside the cell was investigated. It was shown that in the absence of the latter ANS binding in de-energised bacteria occurs mainly at the outside surface.

[Characteristics of copper-induced streptomycin transport in Escherichia coli].

Copper dependent uptake of streptomycin by resting E. coli cells was studied. It was shown that copper stimulates the aminoglycoside uptake only when bacteria possess endogenic energy sources.

[Distribution and changes in properties of ferromagnetic iron particles administered to the animal body].

The registration of ESR signals of organs and tissues in the wide range of temperatures permits to study properties and distribution of ferromagnetic particles in animal organisms. High dispersed powder (HDP) of iron (particle dimension--50-100 nm) was administered subcutaneously to mice in the doses 2 and 100 mg/kg weight of animals. One week after the administration HDP was accumulated in the animal organs under study.

[The effect of highly-dispersed zinc powder on the functional state of blood components and rat liver mitochondria].

The functional state of blood components and liver mitochondria have been investigated by means of ESR method and atomic adsorption spectroscopy. The experimental data indicated that injection of highly dispersed Zn-powder in the concentration of 5 mg/kg of weight did not result in disturbance of mitochondria bioenergetic functions, although the functional state of blood components and the level of metal ions in it were changed.