[Purification of recombinant DNA methyltransferase M2.BstSE from nickase-modification system NM.BstSEI and study of the enzyme properties].

The operon of nickase-modification system from Bacillus stearothermophilus SE-589 (recognition site 5'-GAGTC-3') includes two DNA methyltransferase genes: bstSEIM1 and bstSEIM2. Gene encoding DNA methyltransferase M2.BstSEI was cloned in pJW vector and expressed in E.

[Gene cloning, comparative analysis of the protein structures from Fsp4HI restriction-modification system and biochemical characterization of the recombinant DNA methyltransferase].

Genes coding for the restriction-modification system Fsp4HI, recognizing the sequence 5'-GCNGC-3' have been cloned in Escherichia coli ER2267 cells and its primary structure has been determined. This RM system consists of two genes: the DNA-methyltransferase gene which is followed by the restriction endonuclease gene in the same direction. The analysis of amino acid sequences of the proteins showed that M.