[The effects of simulated microgravity on the pattern of gene expression in human bone marrow mesenchymal stem cells under osteogenic differentiation].

It is well-known that the modified gravity affects not only the organism as a whole, but also the molecular processes in individual cells. The goal of this study was to investigate the influence of simiulated microgravity (SMG) on gene expression pattern in cultured human bone marrow mesenchymal stem cells (hMSCs) after prolonged exposure to SMG on dRPM. It was found that the expression level of 144 genes significantly changed in hMSCs after 20 days of exposure to SMG: the expression of 30 genes significantly increased and 114 - decreased.

[The role of multipotential mesenchymal stromal cells in adaptation of bone marrow precursors osteogenic cell type to microgravity].

Morphofunctional state and differential potential of cultured osteoblasts and osteogenic precursor's cells under real and simulated microgravity are in the focus of presented review. Summary of data of osteoblast cell responses to altered microgravitational circumstances are discussed. Mesenchymal stromal cells are the population of multipotential precursors of adult bone marrow stroma able to differentiate into osteogenic.

[Cytoskeleton structures and adhesion properties of human stromal precursors under conditions of simulated microgravity].

Cytoskeletal alterations occur is several cell types including lymphocytes, glial cells, and osteoblasts, during spaceflight and under simulated microgravity (SMG). One of the potential mechanisms of cytoskeletal gravisensitivity of a cell is disruption of extracellular matrix and integrin interactions. Therefore, we investigated the effects of SMG on F-actin cytoskeleton structure, vinculin focal adhesions, expression of some integrin subtypes and cellular adhesion molecules in mesenchymal stem cells (hMSCs) derived from human bone marrow in vitro.

[Interleukine production in culture of mesenchymal stromal cells of humans during simulation of the microgravity effects].

Effects of simulated microgravity (cell clinostatting and containment in the Random Positioning Machine (RPM), Dutch Space, The Netherlands) on interleukins production by mesenchymal stromal cells (MSC) of the human marrow and MSC osteogenous derivatives obtained through cell stimulation by growth factors (10(-8) M dexamethazone, 0.2 mM ascorbic acid, 10 mM beta-glycerol-phosphate) were studied. Twenty-day clinostatting was found to increase 1.

[New approaches to the immunotherapy of Alzheimer's disease with the synthetic fragments of alpha7 subunit of the acetylcholine receptor].

The effect of immunization with the synthetic fragments of the alpha7 subunit of the acetylcholine nicotine receptor on the spatial memory of mice subjected to olfactory bulbectomy, which causes the development of the neuro-degenetrative disease of Alzheimer's type, was studied. Mice of the NMRI line were immunized with the KLH conjugates of two peptide fragments of the N-terminal fragment of the alpha7 subunit extraxcellular fragment, subjected to olfactory bulbectomy to cause the development of the neurodegenetrative disease of Alzheimer's type, and then the state of the spartial memory was evaluated. It was shown that 20% of bulbectomized mice immunized with the N-terminal 1-23 fragment exhibited good spatial memory after training.