[Changes in lactate dehydrogenase isoforms in the process of oncogenesis].

Isoenzymes of lactate dehydrogenase were studied by disc-electrophoresis in polyacrylamide gel, and in the clinic--in 1% agar gel. Oncovirus A12 invasion of the culture of rat embryo fibroblasts (REF) was found to result in the increased percentage of the cathode fractions activity (LDG-4 and LD-5) and in the disappearance of LDG-1 yet during the first day of the experiment prior to hypoxia and enhanced proliferation, i. e.

[Changes in the isoenzymatic spectrum of energy metabolism enzymes and in the mitochondrial ultrastructure in viral carcinogenesis].

The dynamics of redistribution of the isoenzymatic spectrum of lactate and malate dehydrogenases in the cells of rat embryo fibroblasts (REF) having been in contact with human adenovirus type 12, as compared to the dynamics of changes in the fine structure of the cellular mitochondria of the same models was studied. The action of oncogenic virus caused rearrangment in the isoenzymatic spectra of lactate and malate dehydrogenases both in the nuclei and in the cytoplasm. These disturbances occur from the first days of the REF culture interaction with the oncogenic virus and are not connected with the proliferative growth of the cell culture, but they are stipulated by the very fact of the virus influence on the cell.

[Hexokinase activity in the soluble fraction of the nucleus and cytoplasm in cell cultures infected with human adenoviruses types 6 and 12].

A study was made of the activity of hexokinase in the soluble fraction of the nucleus and cytoplasm of fibroblasts of rat embryos (primary cultures) during contact with infectious (A6) and oncogenic (A12) human adenoviruses. A high activity of the enzyme was seen in the cytoplasmic fraction in comparison with the nuclear one both in the infectious adrenovirus of type 6 this value differed from the normal and in the experimental samples. With the action of the normal but insignificantly.

[Activity of hexokinase isoenzymes in the nuclei of cell cultures infected with oncogenic type-12 adenovirus].

The isoenzymic spectrum of a glycolytic enzyme-hexokinase (HK. 2,7.1.

[Isoenzymes of lactate and malate dehydrogenases in nuclei of rat embryo fibroblasts infected with oncogenic adenovirus type 12].

The isoenzymatic spectrum of lactatedehydrogenase (LDG; L-lactate: NAD-oxireductase; EC 1.1.1.