[Hydrogenase activity in Brevibacterium flavum].

The activity of hydrogenase was assayed in the intact cells and subcellular fractions of Brevibacterium flavum. The organism was shown to have the membrane-bound form of hydrogenase. The soluble NAD+-reducing hydrogenase was not found.

[Cyanide-resistant oxygen consumption of the lysine-synthesizing bacteria Brevibacterium flavum 22 LD].

The growth of the culture and biosynthesis of lysin were studied in Brevibacterium flavum 22 LD cultivated in a chemostat. During cultivation the flow rate of the medium and the partial pressure of oxygen dissolved in the medium were varied. The maximum yield of lysine, calculated in respect to the sucrose consumed, (Yp = g lysine .

[Coordinated regulation of the enzyme activity of glucose transport and metabolism in Brevibacterium flavum].

A coordination was established between the rate of glucose transport and the activity of enzymes involved in glycolysis and the citric acid cycle (CAC) in Brevibacterium flavum cells producing lysine: the rate of glucose transport and the activity of enzymes catalysing glycolysis increased when the activity of CAC enzymes decreased and vice versa. The activity of glycolytic enzymes and the rate of glucose transport in the cells increased also when electron transport through the respiration chain was inhibited and when oxidation and phosphorylation in the respiration chain were uncoupled. The rate of ATP synthesis in the cells is presumed to coordinate the rate of glucose transport and the activity of glycolytic and CAC enzymes in the cells.

[Sensitivity of the oxygen absorption process in Brevibacterium flavum to pH changes in the medium and the action of an oxidation and phosphorylation uncoupler].

The rate of oxygen uptake (QO2) was shown to increase in the chemostat culture of a Brevibacterium flavum 22 LD auxotrophic strain at D = 0.13 h-1 after addition of chlorocarbonylcyanide phenylhydrazone. The index of respiration control reached 1.

[Alternative oxidation pathways in the respiratory chain of Brevibacterium flavum].

A scheme is proposed for the branched respiratory chain in Brevibacterium flavum 22 LD. Three branching sites in the main electron transport pathway are suggested: at the initial stage of NADH2 oxidation (before menaquinone) and at the levels of cytochromes b and c. Up to 40% out of the total oxygen is consumed in the NADH2-dependent alternative oxidative pathway in the presence of 5.