Publications by authors named "Iu D Ignatov"

The most common clinical type of multiple sclerosis (MS), affecting up to 85% of patients living with this disease, is relapsing remitting(RRMS). Relapses are associated with significant functional impairment and decreased quality of life. Residual symptoms after MS relapses may persist and lead to sustained disability.

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RGPU-207 compound and amiodarone in concentrations of 1, 10, 100 and 1000 microM produce dose-dependent and reversible effects on trans-membrane sodium, calcium, and potassium ion currents of neurons in pond snail and orb snail shellfish. In concentration of 1 microM, both compounds increased the amplitude of potassium ion currents, while not affecting the amplitude of sodium and calcium ion currents. In concentrations of 100 and 1000 microM, dose-dependent suppression of all currents (with predominant potassium ion current suppression) was observed.

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Changes in the membrane rest potential (RP), action potential (AP) and impulse activity (IA) in neurons of isolated central nervous system of Lymnaea stagnalis and Planorbarius corneus under the action of anxiolytic afobazole (2-mercaptobenzimidazole derivative) in 1, 10, 100 and 1000 microM concentrations have been studied using a microelectrode technique. It is established that afobazole produces a two-phase, dose-dependent and reversible effect on the electrical activity of neurons. Hyperpolarization by 5-8 mV in the first phase (1-100 microM) and depolarization by 8-10 mV in the second phase (1000 microM) have been observed.

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The new glutamic acid derivative RGPU-135 (3-phenylglutamic acid hydrochloride, glutaron, neuroglutamin) produces dose-dependent and reversible modulation of transmembrane sodium, potassium and, to a greater extent, calcium ion currents in neurons of Lymnaea stagnalis and Planorbarius corneus mollusks at concentrations of 1, 10, 100, and 1000 microM. At concentrations within 1 - 10 microM micromole, Ca and K currents are activated rather insignificantly; at 100 pmole, the amplitude of calcium currents is increased by 5 - 10%; and at 1000 microM, the Na and K ion currents are suppressed by 5 - 12%. RGPU-135 does not influence the membrane surface charge potential and the gating of ion channels.

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Neurophysiological experiments on anesthetized rats were used to study the effects of various doses (12.5, 25, 37.5 mg/kg, i.

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The results of endocellular dialysis membrane potential monitoring show that cardiac glycosides digoxin and corglycon in concentration from 10(-12) to 10(-6) M influence the sodium, calcium and potassium ion currents in isolated snail (Lymnaea stagnalis) neurons in reversible and slightly dose-dependent manner. In low concentration (10(-12) - 10(-8) M) both substances increased all ionic currents by up to 5%, while in higher concentrations both drugs led to the suppression of ion currents. The most pronounced decrease (up to 26% relative to control) was observed for sodium ion current under the action of digoxin in a concentration of 10(-6) M.

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There is extensive clinical evidence for the high efficacy of GABA-ergic drugs in prophylactic and abortive treatment of migraine and cluster headache, while the mechanisms of anticephalgic drugs action are not clear, in particular, because of insufficient number of investigations on experimental headache models. In this study, the influence of baclofen (i.v.

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Efficacy and safety of the following antidepressants: amitryptiline (tricyclic antidepressant), escitalopram (selective inhibitor of serotonin reuptake), milnacipran (selective inhibitor of serotonin and noradrenalin reuptake) and maprotiline (selective inhibitor of noradrenalin reuptake) have been compared during the treatment of 120 patients with chronic daily headache. A positive effect was found for all drugs, in particular in the presence of depressive and anxiety symptoms. Most effective proved to be milnacipran and amitryptiline.

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Experiments on isolated neurons of Lymnaea stagnalis and Planorbis corneus using the intracellular dialysis and fixed membrane potential techniques showed that strophanthin G in a concentration range from 10(-11) to 10(-6) M produce dolse-dependent reversible suppression of the ion currents in potassium, calcium, and sodium channels. At the maximum concentration (10(-6) M), the maximum suppression (up to 55% of that in the control) was observed for the calcium ion current. At the minimum concentration (10(-12) M), the drug did not influence the sodium ion current, but slightly (approximately by 5%) increased the calcium and potassium ion currents.

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Changes of the transmembrane potassium, calcium, and sodium ion currents in isolated neurons of Lymnaea stagnalis snail under the action of ecdysteroid fraction (serpisten) from Serratula coronata L. applied from outside in 0.01 -1000 microg/ml concentrations have been studied using the voltage-clamp technique.

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The effect of anxiolytic afobazole (2-[2-morpholino)ethylthio]-5-ethoxybenzimidazole dihydrochloride) at concentrations of 1, 10, 100 and 1000 microM on transmembrane calcium, sodium, and potassium (slow and rapid) ion currents in isolated neurons of Lymnaea stagnalis mollusks have been investigated. Afobazole was found to produce a dose-dependent reversible action: the drug increased all ion currents at concentrations below 100 microM and inhibited all ion currents at concentrations from 100 to 1000 microM. Afobazole at high concentrations accelerated the inactivation of slow potassium ion current and reversibly inhibited the fast potassium ion current.

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Intracellular dialysis and fixed membrane potential techniques were used to study the extracellular influence of antiarrhythmic drugs--brazidole (a new derivative of 2-mercaptobenzimidazole), amiodarone, sotalol, and hydroxyzine (a tranquilizer) in concentrations 1, 10, 100 and 1000 microM--on the slow potassium ionic transmembrane current in isolated neurons of Lymnnaea stagnalis mollusks. All drugs produced a doze-dependent and reversible suppression of the potassium ion current and accelerated the inactivation kinetics. With respect to the degree of current suppression at 100 microM concentration, the preparations under study can be arranged in the following order: brazidole = hydroxyzine > amiodarone > sotalol.

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Using clamp method it had been shown that He-Ne laser irradiation of the snail neurons increases the amplitude of voltage-gated slow potassium currents in dose of 0.7 x 10(-4) (fluence 1.5 x 10(2) Wt/m2) and decreases it in dose 0.

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Teaching surgery to students of the medical college represents a complex dynamic process based on the achievements and advantages of domestic medical education. The article presents an analysis of historical aspects of teaching surgery formed under the influence of N.I.

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Aseptic inflammation of tissues surrounding large meningeal blood vessels, e.g. the superior sagittal sinus, underlies pathogenesis of migraine.

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On the basis of comprehensive experimental and clinical research the authors defined a variety of migraine-related mechanisms and schemes of migraine-correction by drugs, which should be both of the vascular- and general-actions to ensure an effective medication.

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The circadian and high-frequency (2-2.5 h) pain sensitivity rhythms were observed in male mongrel rats under natural illumination conditions (LD 24, January-February). A minimum pain sensitivity threshold with respect to subcutaneous electrode stimulation was observed in the dark phase of the LD 24 cycle.

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Subchronic administration of tropoxin (in doses of 7.5 and 10 mg/kg) caused dose-dependent blocking of 131I-albumin plasma transudation from the dura mater vessels, induced by electrical stimulation of the trigeminal ganglion and intravenous infusion of the agonist of 5-HT2B/2C receptors metachlorophenylpiperazine. The antimigraine agent metisergid produced a similar effect.

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