[Influence of cultivation temperature of Yersinia pseudotuberculosis on the immunobiological properties of lipopolysaccharide].

Aim: To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS).

Materials And Methods: LPS producing strain--Yersinia pseudotuberculosis 164/84 OX; experimental animal--guinea pigs, rabbits; methods--immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods.

Results: It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains.

[The structural-functional changes in the cells of Francisella tularensis strain 15 Gaĭskiĭ during cultivation and sublimational drying].

Instrumental methods of investigation were used for the demonstration of changes in the fatty acid composition of F. tularensis, strain 15 Gaĭskiĭ, during cultivation in solid culture medium, storage after lyophilization, as well as changes in the functioning of the system of membrane-dependent enzymes of the respiratory chain and in the permeability of cell wall membranes by water molecules and NADH after lyophilization. A relationship between the survival rate of F.