[An experience of the use of stem cells in the treatment of patients with myocardial infarction and low ejection fraction].

We present our own experience of assessment of effect of cell therapy on functional state of the myocardium in patients with lowered contractile capacity of the left ventricular myocardium. Intracoronary administration of stem cells in acute myocardial infarction is a safe method of treatment. It does not cause additional damage of the myocardium and does not provoke appearance of malignant arrhythmia.

[Effect of cytarabine on expression of cell adhesion molecules and on endothelium-leukocyte interaction in vitro].

Aim: To characterize cytarabine effects on human endothelium expression of cell adhesion molecules (CAM) of various classes and interaction of cultured endothelial cells (EC) with blood leukocytes.

Material And Methods: Primary cultures of EC of human umbilical vein were obtained by EC culturing for 24-72 h with cytarabine (10 mcg/ml). They were investigated with phase-contrast microscopy, immunohistochemistry and flow cytometry.

[Space and time organization of perception in patients with chronic cerebral vascular insufficiency].

According to A.A. Ukhtomsky, chronotope is defined as a complex of time and space relations between subjects and events that surround human organism.

[Damage of vascular endothelium in the treatment of acute leukemia: in vitro study].

Aim: Cytostatic drugs used in the treatment of hemopoietic malignancies affect cells of other organs and systems, vascular endothelium, in particular. This leads to cardiovascular complications. We studied effects of some drugs and their combinations on cultured human endothelial cells (EC).

[Effects of ozone on the lipid peroxidation--antioxidation system in blood serum of patients with chronic bronchitis].

Biological effects of ozone on the lipid peroxidation (LPO)--antioxidation system was studied in blood serum of patients with various forms of chronic bronchitis. Breathing ozone was shown to intensify LPO processes and lead to a more considerable deviation of the parameters of non-enzymatic but not enzymatic antioxidants in patients.

[Peripheral blood morphology during chronic bronchitis complicated by the effect of ozone].

In workers afflicted with chronic bronchitis (ChB) after breathing ozone leukocytosis gives place to leukopenia against development of lympho-, monocyto- and neutropenia. In contrast to the workers, in citizens who have not previously contacted ozone chronic obstructive bronchitis is characterized by leukocytosis with less expressed lympho- and neutropenia. Changes in peripheral blood in ChB patients (development of leuko-, lympho-, monocyto- and neutropenia) grow drastically in 5-8 years of ozone inhalation.

[Morphological and functional study of human endothelium response to hyperthermia in vitro].

Effects of hyperthermia (+42.5 degrees C) on the endothelial monolayer organization, actin cytoskeleton and cell viability have been examined in culture of human aortic endothelial cells (EC). Short-lasting hyperthermia (1-4 hrs) provoked disappearance of stress fibers, redistribution of actin filaments to the area of cell-to-cell contacts, shape changes and reorganization of the monolayer.

[Alterations in actin cytoskeleton and rate of reparation of human endothelium (the wound-healing model) under the condition of clinostatting].

Effects of long-term simulation of hypogravity on actin cytoskeleton and cell migration were investigated in cultured human endothelium cells (EC). In control, F-actin resided predominantly on the periphery of cell forming an array of parallel bundles with "dense bodies" along the edge. A small number of actin cable fibers was found in the center.

[On some basic issues and factorial structure of ecological pulmonology].

Consideration is given to the place occupied by diseases of respiratory organs in the general structure of morbidity. In many ways, growth in their number has been determined by amplification of anthropogenic factors. This is manifested in emergence of new eco-dependent diseases and syndromes including more difficult course of pulmonary pathology.

[Gravitation sensitivity of human endothelium].

Experiments on the effects of hypogravity (clinostatting) on growth and formation of monolayer of cultivated endothelium cells of the human umbilical vein demonstrated sensitivity of endothelium to the gravitational stimulus as it responded by a significant reduction of the proliferative activity of cells in culture. The most favorable conditions for cultivating endothelium cells under extended (15-d) hypogravity in vitro were determined.

[Spatial and temporary changes in sensitivity of murine proliferative system and energy exchange to typhoid fever infection].

The authors studied the spatial and temporary organization of the proliferative system and energy exchange in the small intestinal epithelium, as well as spatial and temporary changes in the sensitivity of these systems in mice to typhoid fever infection (cultured Salmonella typhi, 4446) at day and night. The small intestinal epithelial systems were found to show a spatial and time organization and a close correlation between their temporary and spatial changes after infection. The temporary organization of the proliferative system in the esophageal epithelium is more sensitive to infection in the day-time and that of the small intestinal epithelial proliferative system is more marked at night.

[From chronobiology to chronotopobiology].

Based on the data available in the literature and his own findings, the author presents a concept of the spatial-temporary organization of biological systems. Experimental evidence is provided for this concept with emphasis on its theoretical and medical values. A role of spatial and temporary organization in the activity of the biological system is shown separately.

[Chrono-ecological analysis of population health and ways of its recreation].

Reported are results of multiyear observation of the structure of individual and population health in Vladikavkaz, and the authors' concept of health recovery and protection through application of modern high technologies of active chronoadaptation to extreme living conditions. The proposed methods of active chronoadapation, i.e.

[Role of staphylococcus aureus hemolytic toxin-alpha in pathogenesis of infectious endocarditis: studies in vitro].

Aim: To study effects of alpha-toxin (AT) Staphylococcus aureus on human platelets and endothelial cells.

Materials And Methods: Concentrations of intracellular calcium in human platelets and endothelial cells were estimated by fluorescence, phosphoinositide metabolism in the endothelial cells was studied using 3H-myoinositole.

Results: AT induced a dose-dependent increase of intracellular calcium in blood and vascular cells, stimulates dose-dependent formation of inositol phosphates in endothelial cells.

[Coculture of human endothelium and smooth muscle cells: functional heterogeneity of endothelial cells from zones with different susceptibility to atherosclerosis].

Using co-culture technique and 3H-thymidine radioautography we have studied effects of human aortic endothelial cells (EC), isolated separately from zones of low (LP) and high (HP) probability of atherosclerosis of grossly normal and atherosclerotic aortas, on 3H-thymidine incorporation by human intimal smooth muscle cells (SMCs). It was found that EC activity depended on a zone of probability, from which the cells were isolated, and on the degree of atherosclerotic lesion. The first-passage ECs from grossly normal LP zones inhibited 3H-thymidine incorporation, compared to control, incubated without Ecs, SMCs (63.

[Induction of cell adhesion molecules, E-selectin, VCAM-1 and ICAM-1, in a co-culture of human endothelial and smooth muscle cells].

Using immunofluorescence and flow cytometry, we studied the surface expression of cell adhesion molecules, E-selectin, VCAM-1 and ICAM-1, in human umbilical vein endothelial cells (HUVEC) co-cultured with human aortic intimal smooth muscle cells (SMC). It was found that inactivated HUVEC constitutively expressed only ICAM-1. After 3-4 h of co-culturing with SMC in the Transwell system we observed the appearance of E-selectin and VCAM-1, and the increase of ICAM-1 content on the cell surface.

[Morphologic and functional characterization of human aortic endothelium. III. Growth in culture and colony formation at low seeding density].

In human aortic endothelial cell (HAEC) cultures, obtained separately from aortic zones of low (LP) and high (HP) probability of atherosclerosis, proliferative characteristics of cells and HAEC ability to form colonies at clonal seeding density were studied. It has been found that the population doubling time is significantly higher in endothelial cell (EC) cultures from HP-zones, compared to that in cultures from LP-zones of the same vessels. In cultures from both LP- and HP-zones only a few percent of EC had a proliferative potential enough to form cell colonies.

[A chronobiological study of the function of the epithelial proliferative system of the murine esophagus after its infection with typhoid and the use of lomefloxacin].

The rhythmical character of the function of the proliferative system of the oesophagus epithelium of mice changed after infection by Salmonella typhi and after injection of lomefloxacin to intact and infected mice. The degree of the changes depended on the exposure time. The proliferative system of the oesophagus epithelium was more resistant to the infection in the night-time than the day-time and equally resistant to the effect of lomefloxacin in both the day-time and the night-time.

[Interaction of a monoclonal antibody to P-selectin with activated platelets and endothelial cells. Heterogeneity of expressing P-selectin in human aorta endothelial cells].

Monoclonal antibody (MAb) CRC81 against P-selectin, a membrane cell adhesion protein of platelets and endothelial cells (EC), has been obtained and characterized. The antibody selectively interacted with the surface of activated platelets and EC due to the redistribution of P-selectin from intracellular organelles onto the surface by activation. MAb CRC81 could recognize not only human but also rabbit and dog P-selectins.

[Temporal organization and information in biological systems].

Analysis of the available chronobiological data leads to the following conclusions about the role of biorhythms in the informational interface in living systems: (1) temporal organization of living systems is the main channel of informational interface in organism (the principle of the bond between information and temporal organization); (2) biorhythms or, more accurately, temporal organization of type one postulates the fulfillment of one of the fundamental statements of the general information theory, i.e., correspondence of information to the substratum of its application (the correspondence principle); (3) the biorhythm phases can either unidirectionally strengthen, weaken or realize in different directions the biological potentials of information brought into organism by one and the same material carrier (the principle of amplification, attenuation or inversion of informational potential by biorhythm); (4) biorhythm is the mechanisms driving the transformation of continuous informational signal, if any, into discrete, i.

[Antiendothelial antibodies in systemic scleroderma and Raynaud's disease].

Using ELISA on fixed endothelial hybridoma (EAhy.926) cells we investigated the occurrence of antiendothelial antibodies (AEA) in the sera from patients with scleroderma systematica (SS) (n = 70) and Raynaud's disease (RD) (n = 19). The mean IgG and IgA-AEA levels were significantly higher in the SS patients than RD patients (p < 0.